Recent advances in electrochemiluminescence immunoassay based on multiple-signal strategy

Du, Fangxin; Chen, Yequan; Meng, Chengda; Lou, Baohua; Zhang, Wei; Xu, Guobao

doi:10.1016/j.coelec.2021.100725

Cited by 50 publications

(40 citation statements)

References 56 publications

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“…Luminol is one of most popular ECL luminophores because of its superior features such as low cost, high ECL quantum efficiency in aqueous solutions, and broad spectrum of applications. It has been used in the direct detection of H 2 O 2 , the indirect detection of enzymes and their substrates through the detection of H 2 O 2 , and so on. ,,, Therefore, it is important to find electrodes that can realize the sensitive determination of H 2 O 2 . However, almost all bare electrodes have limited sensitivity, and chemically modified electrodes have the disadvantage of relatively complex modification.…”

Section: Exploration Of Novel Electrodes For Ecl Devicesmentioning

confidence: 99%

“…Electrochemiluminescence (ECL), also called electrogenerated chemiluminescence, is a luminescence process whereby excited states of luminophores are electrochemically generated in the vicinity of the electrode surface and then relax to a lower state, leading to light emission. − By dexterously combining the electrochemical and chemiluminescence strategies, ECL inherits the fascinating merits of both techniques, such as high sensitivity, a wide dynamic range, and easy multiplex imaging analysis of chemiluminescence as well as good stability and tunability of electrochemical methods. The appealing temporal and spatial control of light emission makes the ECL technique a very powerful platform for both sensing and imaging. − In fact, ECL has been extensively studied in many areas of analytical chemistry including immunoassays, , environmental detection, aptasensors, and so on, benefiting from the exploration of novel luminophores, , coreactants, , accelerators, electrode materials, , and innovative devices , in the ECL detection process.…”

Section: Introductionmentioning

confidence: 99%

“…The appealing temporal and spatial control of light emission makes the ECL technique a very powerful platform for both sensing and imaging. 8−10 In fact, ECL has been extensively studied in many areas of analytical chemistry including immunoassays, 10,11 environmental detection, 12 aptasensors, 13 and so on, benefiting from the exploration of novel luminophores, 14,15 coreactants, 16,17 accelerators, 18 electrode materials, 19,20 and innovative devices 21,22 in the ECL detection process.…”

Section: Introductionmentioning

confidence: 99%

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Rational Design of Electrochemiluminescent Devices

Gao

et al. 2021

Acc. Chem. Res.

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119

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Metrics & MoreArticle Recommendations CONSPECTUS: Electrochemiluminescence (ECL) is a light-emitting process which combines the intriguing merits of both electrochemical and chemiluminescent methods.It is an extensively used method especially in clinical analysis and biological research due to its high sensitivity, wide dynamic range, and good reliability. ECL devices are critical for the development and applications of ECL. Much effort has been expended to improve the sensitivity, portability, affordability, and throughput of new ECL devices, which allow ECL to adapt broad usage scenarios.In this Account, we summarize our efforts on the recent development of ECL devices including new electrodes, ECL devices based on a wireless power transfer (WPT) technique, and novel bipolar electrochemistry. As the essential components in the ECL devices, electrodes play an important role in ECL detection. We have significantly improved the sensitivity of luminol ECL detection of H 2 O 2 by using a stainless steel electrode. By using semiconductor materials (e.g., silicon and BiVO 4 ), we have exploited photoinduced ECL to generate intense emission at much lower potentials upon illumination. For convenience, portability, and disposability, ECL devices based on cheap WPT devices have been designed. A small diode has been employed to rectify alternating current into direct current to dramatically enhance ECL intensity, enabling sensitive ECL detection using a smart phone as a detector. Finally, we have developed several ECL devices based on bipolar electrochemistry in view of the convenience of multiplex ECL sensing using a bipolar electrode (BPE). On the basis of the wireless feature of BPE, we have employed movable BPEs (e.g., BPE swimmers and magnetic rotating BPE) for deep exploration of the motional and ECL properties of dynamic BPE systems. To make full use of the ECL solution, we have dispersed numerous micro-/nano-BPEs in solution to produce intense 3D ECL in the entire solution, instead of 2D ECL in conventional ECL devices. In addition, the interference of ECL noise from driving electrodes was minimized by introducing the stainless steel with a passivation layer as the driving electrode. To eliminate the need for the fabrication of electrode arrays and the interference from the driving electrode and to decrease the applied voltage, we develop a new-type BPE device consisting of a single-electrode electrochemical system (SEES) based on a resistance-induced potential difference. The SEES is fabricated easily by attaching a multiperforated plate to a single film electrode. It enables the simultaneous detection of many samples and analytes using only a single film electrode (e.g., screen-printed electrode) instead of electrode arrays. It is of great potential in clinical analysis especially for multiple-biomarker detection, drug screening, and biological studies. Looking forward, we believe that more ECL devices and related ECL materials and detection methods will be developed for a wide range of applications, such as in v...

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Section: Exploration Of Novel Electrodes For Ecl Devicesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Rational Design of Electrochemiluminescent Devices

Gao

et al. 2021

Acc. Chem. Res.

Self Cite

119

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show abstract

“…[18][19][20][21][22][23][24][25] Among these methods, ECL is the method widely used in various bioanalyses due to its simplicity, high sensitivity, wide dynamic range, and low background interference. [26] An aptamer is a single-stranded or double-stranded oligonucleotide fragment obtained from artificially constructed random singlestranded oligonucleotide (ssDNA or ssRNA) libraries through ligand index enrichment phylogenetic evolution technology. [27] Aptamers have an ability to recognize various targets, can be easily prepared, have higher thermal stability and are more controllable.…”

Section: Introductionmentioning

confidence: 99%

A sensitive electrochemiluminescent sensor chip based on the ssDNA‐Ru(II) complex and aptamer for the determination of thrombin

Luo

Song

et al. 2022

Luminescence

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In this work, an electrochemiluminescence (ECL) sensor chip for sensitive detection of thrombin (TB) was prepared using a screen-printed electrode (SPE) as a working electrode and an aptamer as a specific recognition moiety. To produce an ECL sensor chip, a layer of pL-Cys was immobilized on the surface of the SPE using the cyclic voltammetry scanning method. A layer of gold nanoparticles (AuNPs) was assembled through an Au-S bond and hairpin DNA was further immobilized on the electrode surface. Ru(bpy) 2 (mcpbpy) 2+ , as a luminescent reagent, was covalently bound to single-stranded DNA (ssDNA) to prepare a luminescence probe ssDNA-Ru. The

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“…Most ECL ratiometric strategies are based on the first mode. In this case, two ECL luminophores are either encapsulated independently and introduced into the ECL system in steps, , or encapsulated as a composite and introduced into the ECL system simultaneously . For example, Xu’s group separately introduced Au-g-C 3 N 4 and Ru(bpy) 3 2+ to construct an ECL ratio biosensor for detecting miRNA-21 through the resonance energy transfer (RET) between two luminophores .…”

Section: Introductionmentioning

confidence: 99%

Novel Ratiometric Electrochemiluminescence Biosensor Based on BP-CdTe QDs with Dual Emission for Detecting MicroRNA-126

Zhao

Tan

et al. 2021

Anal. Chem.

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The electrochemiluminescence (ECL) ratiometric assay is usually based on two different ECL luminophores, and the choice of two suitable luminophores and shared co-reactant makes its construction challenging. The single-emitter-based ECL ratio mode could overcome the limitation of two luminophores and simplify the construction process, so it is an ideal choice. In this work, CdTe quantum dots (CdTe QDs) were modulated using black phosphorus (BP) nanosheet to simultaneously emit the cathodic and anodic ECL signals, and H 2 O 2 and tripropylamine (TPrA) served as the cathodic and anodic co-reactants, respectively. MicroRNA-126 (miRNA-126) was selected as the template target to exploit the application of BP-CdTe QDs in the single-emitter-based ECL ratio detection. Through the target recycling triggering rolling-circle amplification (RCA) reaction, a large amount of glucose oxidase (GOx)-modified single strand 1 was introduced. GOx catalyzed glucose to produce H 2 O 2 in situ, which acted as a dual-role moderator to quench the anodic ECL emission with TPrA as the co-reactant while enhancing the cathodic emission, thereby realizing the ratiometric detection of miRNA-126 with a low detection limit of 29 aM (S/N = 3). The dual-ECL-emitting BP-CdTe QDs with TPrA-H 2 O 2 as dual co-reactant provide a superior ECL ratio platform involving enzyme catalytic reaction, expanding the application of single-emitter-based ratio sensing in the diverse biological analysis.

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Recent advances in electrochemiluminescence immunoassay based on multiple-signal strategy

Cited by 50 publications

References 56 publications

Rational Design of Electrochemiluminescent Devices

Rational Design of Electrochemiluminescent Devices

A sensitive electrochemiluminescent sensor chip based on the ssDNA‐Ru(II) complex and aptamer for the determination of thrombin

Novel Ratiometric Electrochemiluminescence Biosensor Based on BP-CdTe QDs with Dual Emission for Detecting MicroRNA-126

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