A sensitive electrochemiluminescent sensor chip based on the ssDNA‐Ru(II) complex and aptamer for the determination of thrombin

Abstract

In this work, an electrochemiluminescence (ECL) sensor chip for sensitive detection of thrombin (TB) was prepared using a screen-printed electrode (SPE) as a working electrode and an aptamer as a specific recognition moiety. To produce an ECL sensor chip, a layer of pL-Cys was immobilized on the surface of the SPE using the cyclic voltammetry scanning method. A layer of gold nanoparticles (AuNPs) was assembled through an Au-S bond and hairpin DNA was further immobilized on the electrode surface. Ru(bpy) 2 (mcpbpy) 2+ , as a luminescent reagent, was covalently bound to single-stranded DNA (ssDNA) to prepare a luminescence probe ssDNA-Ru. The

Bottlebrush DNA-Primed Rolling Circle Amplification for Sensitive Detection of Biomolecules

“…Clearly, ultrasensitive detection of biomolecules, especially those of low abundance, can be achieved with this BDP-RCA-based detection method, and the detection sensitivity can still be further increased by associating the BDP-RCA with other advanced means for signal generation, such as electrochemiluminescence and electrochemical methods, instead of fluorescence of the stained BDP-RCA product. , The RCA technique has been widely used for signal amplification in biosensing, − and the BDP-RCA method developed in this work produces the amplification products much more rapidly than the regular RCA, which significantly increases the gain of the signal in a limited time, and greatly shortens the time needed for detecting a certain target. Although a similar approach, branched RCA technique has also been developed recently and used in several detection applications, , in branched RCA, the side DNA chains in the product are attached to the main DNA chain via hybridization of the complementary sequences.…”

A perovskite‐based electrochemiluminescence aptasensor for tetracycline screening

“…Here, we synthesized CsPbBr 3 NCs with cetyltrimethylammonium bromide (CTAB) as a ligand (CTAB‐PNC), which produced strong and stable cathodoluminescent signals in water. An aptamer is an oligonucleotide composed of a single strand of DNA or RNA [29]. As a biorecognition element, aptamers exhibit high specificity, stability, and ease of synthesis and modification.…”