Recent advances in carbohydrate-based vaccines

Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase

“…S1). No mass modification was found on the Endo H-treated chymotryptic peptides containing Asn 8 and Asn 29 , demonstrating the absence of glycosylation of these putative sites in the wild-type XynA. In these experiments, the sequence coverage was 100%, and the N-terminal peptide analysis revealed that the signal sequence peptidase present in P. pastoris cleaved the protein three residues upstream from the expected Kex2 signal peptide cleavage site (31).…”

“…The biosynthesis, molecular composition, and functions of glycoproteins are well documented, and glycans can act as recognition markers (5-7); mediators of biomolecular interactions (8,9); immune response modulators (10,11); regulators of protein turnover and protein folding (12,13); and markers for protein secretion (14), intracellular trafficking, and cell communication (13,15). Due to their hydrophilicity and bulk, glycan modification can increase glycoprotein solubility, improve resistance against proteolysis, and reduce non-specific protein interactions (16).…”

Biosynthesis and Biological Activity of Carbasugars

“…Thus, medicinal chemistry techniques can potentially be used to derivatize and modify synthetic carbohydrates to make vaccines that are more immunogenic than those based on natural carbohydrates. For selected, general, and recent reviews on these aspects of carbohydrate chemistry, see (a) general references [2][3][4][5]; (b) synthetic aspects [6][7][8][9]; (c) carbohydrates in biological and medicinal chemistry [10][11][12][13][14]; (d) glycobiology [15]; (e) signaling, cell-cell communication, and molecular and cellular targeting [16][17][18][19][20][21]; (f) therapeutic potential of glycoconjugates [22,23]; (g) carbohydrate-based vaccines [24][25][26][27][28].…”

Hydrolysis and Formation of Glycosidic Bonds

“…In fact, oligosaccharides and various glycoconjugates have been shown to modulate different molecular recognition processes, including viral and bacterial pathogens recognition [1][2][3][4][5], tumor-associated cell adhesion and metastasis events [6][7][8], immune response [9], fertilization [10], and neuronal development [11]. This significant role, together with advances in the glycomics field and the recent developments of new synthetic methodologies, for example, the glycorandomization of biologically active natural products [12,13], makes glycoconjugates extremely attractive therapeutic targets nowadays [14][15][16][17].…”