Recent advances in capillary electrophoresis for biomarker discovery

Bakry, Rania; Huck, Christian W.; Najam-ul-Haq, M.; Rainer, Matthias; Bonn, Günther K.

doi:10.1002/jssc.200600323

Cited by 26 publications

(15 citation statements)

References 80 publications

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“…Those advantages, that were very recently confirmed in detail in several reviews (Song et al, 2008; Bakry et al, 2007), are especially beneficial when analyzing a large number of heterogeneous samples that contain interfering compounds, such as lipids, precipitates, etc. CE’s main advantages are the robustness, ability to recondition fast with NaOH, simple separating principle with high reproducibility, and, with respect to MS interfacing, a buffer that does not change its composition during analysis, as no buffer-gradient is applied.…”

Section: Technical Aspectsmentioning

confidence: 76%

Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments

Mischak

Coon

Novák

et al. 2008

Mass Spectrometry Reviews

175

141

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Proteome analysis has emerged as a powerful technology to decipher biological processes. One of the main goals is to discover biomarkers for diseases from tissues and body fluids. However, the complexity and wide dynamic range of protein expression present an enormous challenge to separation technologies and mass spectrometry (MS). In this review, we examine the limitations of proteomics, and aim towards the definition of the current key prerequisites. We focus on capillary electrophoresis coupled to mass spectrometry (CE-MS), because this technique continues to show great promise. We discuss CE-MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. Finally, we present several examples on the use of CE-MS to determine urinary biomarkers and implications for disease diagnosis, prognosis, and therapy evaluation.

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Section: Technical Aspectsmentioning

confidence: 76%

Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments

Mischak

Coon

Novák

et al. 2008

Mass Spectrometry Reviews

175

141

View full text Add to dashboard Cite

show abstract

“…Additionally, LC is sensitive towards interfering compounds and precipitation of analytes on column materials [13] , complicating separation of large analytes covering broad ranges of hydrophobicity. In the last decade, monoliths were applied in mircoscale chromatographic separation, such as -LC-MS [20] , to tackle these limitations due to easy column preparation, fritless design, high permeability, low backpressure, fast analyte mass transfer, and versatile surface chemistry [21] . In contrast, CE-MS used free-flow separation of analytes in buffer-filled capillaries [22] .…”

Section: Proteomics Platformsmentioning

confidence: 99%

Proteomics in Gerontology: Current Applications and Future Aspects – A Mini-Review

2009

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Background: Aging is closely related to the onset of chronic diseases, such as coronary artery disease, diabetic nephropathy or different types of malignancies, reflecting the demand for novel biomarkers to manage theses diseases. Objective: The analysis of the human proteome for biomarkers has made considerable advances in the last years. Methods: We describe the main technological approaches taken, their advantages and disadvantages. Results: We will review the different clinical sources of material and attempt to highlight the different challenges and approaches associated with these. Age-related changes in the proteome have been described and were found to be highly similar to changes associated with chronic diseases. We will give several examples on the successful application of proteomics in the diagnosis, prognosis and therapy of these chronic diseases. Conclusions: A boost in disease-related proteomic information is expected in the very near future, and will also result in its broad clinical application. However, this view appears to be dependent on the strict adherence to proper technological/analytical parameters, correct statistics, and large databases that allow comparison of datasets provided by different scientists. Clearly, the proteome is by far too complex to be tackled by one laboratory on its own.

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“…Furthermore, CE allows fast separation and is generally not affected by many compounds that interfere with LC . This results in a platform that is well suited for the analysis of a wide range of bioactive compounds in complex matrices . CE can be coupled to a various range of ionization sources such as MALDI or ESI, with the latter being the most widely used in bioanalytical settings since it offers high sensitivity and stability .…”

Section: Ce In Comparison To Lcmentioning

confidence: 99%

Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications

et al. 2013

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Proteome analysis using capillary electrophoresis coupled to mass spectrometry (CE-MS) has been used in a number of clinical applications in the past years. The main focus of CE-MS-based studies has been on the investigation of urine, due to the stability of the urinary proteome, ease of collection, and also the low molecular weight range of the urinary proteome, mostly peptides below 30 kDa. The reproducibility of this approach has enabled analysis of over 20 000 samples in a comparable way, giving enormous statistical power to any additional study involving this methodological setup. In this article, we review the major technological issues associated with the application of CE-MS in the routine investigation of the urinary proteome for clinical applications. We pinpoint recent developments that may have a chance to improve on the currently used approach, and highlight obstacles that need to be solved. In the second part of the article, we review the recent clinical applications, aiming to highlight relevant issues, and possible future routine applications in clinical diagnosis. In the end, we provide a short outlook, and indicate future developments to be expected, as well as problems that need to be solved to enable routine application of CE-MS in a clinical setting.

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Recent advances in capillary electrophoresis for biomarker discovery

Cited by 26 publications

References 80 publications

Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments

Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments

Proteomics in Gerontology: Current Applications and Future Aspects – A Mini-Review

Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications

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