Recent Advances in Biological Research Vol. 3

Muturi, S. M.; Wachira, F. N.; Karanja, L. S.; Njeru, L. K.; Samant, Parineeta; Okechukwu, J.; Ike, Anthony C.; Olovo, Chinasa Valerie; Julien, Coulibaly Kalpy; Sabine, Vakou N’dri; David, Coulibaly N’Golo; Joseph, Djaman Allico; Dosso, Mireille; Abiaobo, N. O.; Udo, M. T.; Kator, Liamngee; Iheanacho, A. C.; Aloho, Kortse P.; Zahir, Ilham; Houari, Abdellah; Iraqui, Mohammed; Ibnsouda, Saad; Inala, Mary Shobha Rani; Dayanand, C. D.; Beena, P. M.; Kutty, A V Moideen; Mbim, E; Mboto, C. I.; Agbo, B. E.; Morebise, Olugbenga; Ezepchuk, Yurii V.; Tarh, Jacqueline Ebob; Iroegbu, Christian Ukwuoma; Eze, Ifeyinwa Mirabel; Nwibo, Daniel Don

doi:10.9734/bpi/rabr/v3

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“…The correlation between human/veterinary health and elimination of pathogens has caused researchers to prioritise resistance studies in pathogenic bacteria. 32 The focus on well-established pathogenic bacteria has recorded a considerable improvement in the fight against pathogenic inversion of animals and humans, as more methods for strengthening pathogens detection in the laboratory are increasingly suggested. 33 However, this approach would continue to yield considerable results only to the extent of researchers’ knowledge of the contribution of commensals and under-studied bacteria in the population to the global distribution of resistance genes.…”

Section: Discussionmentioning

confidence: 99%

Occurrence ofPseudomonas lactisandPseudomonas paralactisAmongst Non-Lactose-Fermenting Bacterial Isolates in Chickens and Their Antimicrobial Resistance Patterns

2022

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A serious concern of public-health proportion is rising from the carriage of antibiotic resistance determinant in Non-Lactose-Fermenting Bacteria and acquisition of virulence particularly in strains that are not routinely isolated or screened from common food animals. This study investigated the resistance profile and pathogenicity potential of selected Non-Lactose-Fermenting Bacteria isolated from 18 poultry farms. In total, we investigated the antibiotic susceptibility patterns of 25 Pseudomonas lactis and 71 Pseudomonas paralactis isolated from chicken faeces by testing them against 12 antibiotics. Resistance genes borne by the selected isolates were screened by sequencing the genetic location of resistance determinants was determined by plasmid curing. The virulence potential of the studied strains was determined phenotypically. Pseudomonas lactis isolates were mostly resistant to azetronam (93%), followed by trimethoprim (90%), cefotaxime (86%) and then amoxicillin/clavulanic acid (57%), while Pseudomonas paralactis. isolates were most resistant to azetronam (94%), trimethoprim (90%), cefepime (80%), piperacillin (75%) and amoxicillin/clavulanic acid (70%). The Multiple Antibiotic Resistance Index of Pseudomonas lactis and Pseudomonas paralactis isolates respectively ranged from 0.0 to 0.8 and 0.0 to 0.9. Polymerase Chain Reaction revealed the presence of antibiotic resistance factors such as blaCTX-M, qnrS, aac (6′)-lb-cr and blaSHV while plasmid curing revealed carriages of resistance determinants on Resistance Plasmid. Moreover, virulence enzymes such as alkaline protease and phospholipase C were found in 3% and 12% of Pseudomonas paralactis and Pseudomonas lactis, respectively. This study reports the first occurrence of Pseudomonas lactis and Pseudomonas paralactis strains from chicken faeces, and their antimicrobial resistance and relative virulence suggest the encroachment of food animals by the under-studied non-lactose-fermenting bacteria that should alert public health concerns.

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Section: Discussionmentioning

confidence: 99%