Recent advances in atomic force microscopy for assessing the nanomechanical properties of food materials

Cárdenas-Pérez, Stefany; Chanona‐Pérez, José Jorge; Méndez-Méndez, Juan Vicente; Arzate-Vázquez, I.; Hernández‐Varela, Josué David; Vera, Norma Güemes

doi:10.1016/j.tifs.2018.04.011

Cited by 42 publications

(27 citation statements)

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“…The current–simplified but effective–approach for obtaining the Young’s modulus from single nanoindentation curves is based on linear contact mechanics [ 44 ]. For a spherical indenter, this theory is reduced to the well-known Hertz equation [ 45 ]: where F is the force, the indentation, R the radius of the sphere, E the Young’s modulus and the Poisson’s ratio of the indented material. Using this equation, it is possible to fit the curves and obtain E as a fitting parameter.…”

Section: Resultsmentioning

confidence: 99%

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Elasticity spectra as a tool to investigate actin cortex mechanics

et al. 2020

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young’s modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young’s modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

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Section: Resultsmentioning

confidence: 99%

“…The current-simplified but effective-approach for obtaining the Young's modulus from single nanoindentation curves is based on linear contact mechanics [44]. For a spherical indenter, this theory is reduced to the wellknown Hertz equation [45]:…”

Section: Calculating Elasticity Spectra From Nanoindentation Experimentsmentioning

confidence: 99%

Elasticity spectra as a tool to investigate actin cortex mechanics

et al. 2020

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“…The current -simplified but effective -approach for obtaining the Young's modulus from single nanoindentation curves is based on linear contact mechanics [41]. For a spherical indenter, this theory is reduced to the well-known Hertz equation [42]:…”

Section: Calculating Elasticity Spectra From Nanoindentation Experimentsmentioning

confidence: 99%

Elasticity Spectra as a Tool to Investigate Actin Cortex Mechanics

Lüchtefeld

Bartolozzi

Morales

et al. 2020

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Background The mechanical properties of single living cells have proven to be a powerful marker of the cell physiological state. The use of nanoindentation-based single cell force spectroscopy provided a wealth of information on the elasticity of cells, which is still largely to be exploited. The simplest model to describe cell mechanics is to treat them as a homogeneous elastic material and describe it in terms of the Young's modulus. Beside its simplicity, this approach proved to be extremely informative, allowing to assess the potential of this physical indicator towards high throughput phenotyping in diagnostic and prognostic applications. Results Here we propose an extension of this analysis to explicitly account for the properties of the actin cortex. We present a method, the Elasticity Spectra, to calculate the apparent stiffness of the cell as a function of the indentation depth and we suggest a simple phenomenological approach to measure the thickness and stiffness of the actin cortex, in addition to the standard Young's modulus. Conclusions The Elasticity Spectra approach is tested and validated on a set of cells treated with cytoskeleton-affecting drugs, showing the potential to extend the current representation of cell mechanics, without introducing a detailed and complex description of the intracellular structure.

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“…Readers interested in the applications of AFM to measure nanomechanical properties of vegetable, fruit or meat tissues are encouraged to peruse recent reviews (Cárdenas-Pérez et al, 2019;Posé et al, 2019). Bacteria and yeasts, including food-grade lactic acid bacteria or baker's yeast Saccharomyces cerevisiae, have also been the subject of many mechanical evaluations using AFM indentation (Arfsten, Leupold, Bradtmöller, Kampen, & Kwade, 2010;Bui, Kim, & Choi, 2008;Schaer-Zammaretti & Ubbink, 2003;Touhami, Nysten, & Dufrene, 2003).…”

Section: Elasticity and Stiffnessmentioning

confidence: 99%