Recent advances in antibody-based monolith chromatography for therapeutic applications

Mayolo‐Deloisa, Karla; Rito‐Palomares, Marco

doi:10.1016/b978-0-08-103019-6.00005-9

Cited by 3 publications

(5 citation statements)

References 34 publications

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“…They suited well for monosaccharides and showed high efficiency, although principally aminobonded materials are used for their studies [148,149]. In LC-MS/MS monolithic sorbents allowed the identification and quantification of monoclonal antibodies (mAbs), glycopeptides [15,80], and glycans [62,80]. In particular, polymer-based monolith sorbents were favorable in the quantification of mAbs in mixtures of therapeutic intact (Table S1).…”

Section: 11mentioning

confidence: 99%

“…In particular, polymer-based monolith sorbents were favorable in the quantification of mAbs in mixtures of therapeutic intact (Table S1). Although monolithic materials can also be antibody-activated, without activation they perform more quantitatively for mAbs [62].…”

Section: 11mentioning

confidence: 99%

“…Glycosaminoglycans (GAGs i.e., hyaluronic acid, chondroitin sulfate, dermatan sulfate, heparin sulfate, and heparin, Figure 1) are linear anionic polysaccharides and conjugated with membranes and with glycosylphosphatidylinositol anchored proteins [3,13,14,21]. GAGs may move into extracellular matrices and form bonds with plasma proteins [19,25,61,62]. They have many important biological and pharmacological applications.…”

Section: Homologous and Heterogeneous Oligo-and Polysaccharides And G...mentioning

confidence: 99%

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Research of saccharides and related biocomplexes: A review with recent techniques and applications

Sirén

2024

J of Separation Science

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Saccharides and biocompounds as saccharide (sugar) complexes have various roles and biological functions in living organisms due to modifications via nucleophilic substitution, polymerization, and complex formation reactions. Mostly, mono‐, di‐, oligo‐, and polysaccharides are stabilized to inactive glycosides, which are formed in metabolic pathways. Natural saccharides are important in food and environmental monitoring. Glycosides with various functionalities are significant in clinical and medical research. Saccharides are often studied with the chromatographic methods of hydrophilic interaction liquid chromatography and anion exchange chromatograpy, but also with capillary electrophoresis and mass spectrometry with their on‐line coupling systems. Sample preparation is important in the identification of saccharide compounds. The cases discussed here focus on bioscience, clinical, and food applications.

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Section: 11mentioning

confidence: 99%

Section: 11mentioning

confidence: 99%

Section: Homologous and Heterogeneous Oligo-and Polysaccharides And G...mentioning

confidence: 99%

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Research of saccharides and related biocomplexes: A review with recent techniques and applications

Sirén

2024

J of Separation Science

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“…Monoliths represent the fourth generation of chromatographic matrices, characterized by a unique structure featuring highly interconnected channel networks making them distinct from other supports [ 13 ]. Although first introduced in the 1990s [ 9 ], monoliths have gained prominence as alternatives to traditional packed columns [ 14 ].…”

Section: Monolithsmentioning

confidence: 99%

Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

Ribeiro,

Luís,

Rodrigues

et al. 2024

Gels

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The increasing demand for highly pure biopharmaceuticals has put significant pressure on the biotechnological industry to innovate in production and purification processes. Nucleic acid purification, crucial for gene therapy and vaccine production, presents challenges due to the unique physical and chemical properties of these molecules. Meeting regulatory standards necessitates large quantities of biotherapeutic agents of high purity. While conventional chromatography offers versatility and efficiency, it suffers from drawbacks like low flow rates and binding capacity, as well as high mass transfer resistance. Recent advancements in continuous beds, including monoliths and cryogel-based systems, have emerged as promising solutions to overcome these limitations. This review explores and evaluates the latest progress in chromatography utilizing monolithic and cryogenic supports for nucleic acid purification.

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“…The past decade has witnessed the advancement of monoliths as an important technology in diverse applications including separation [ 1 ], filtration [ 2 ], biomolecule purification [ 3 ] and chromatography systems [ 4 ]. This is owing to their fascinating characteristics, which include high surface area, chemical stability, large pore volumes, high permeability and low flow resistance, which enable high-speed separation, making them promising for achieving high throughput, resolution, and separation in short run times.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Polystyrene Microsphere-Templated Porous Monolith for Wastewater Filtration

et al. 2021

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Porous monoliths prepared using templates are highly sought after for filtration applications due to their good mass transport properties and high permeability. Current templates, however, often lead to the formation of dead-end pores and irregular pore distributions, which reduce the efficiency of the substrate flow across the monolith column. This study focused on the preparation of a microsphere-templated porous monolith for wastewater filtration. The optimal template/monomer ratio (50:50, 60:40, 70:30) was determined, and appropriate template removal techniques were assessed for the formation of homogenous pores. The physicochemical characteristics and pore homogeneity of the monoliths were examined. The 60:40 ratio was determined to result in monoliths with homogeneous pore distributions ranging from 1.9 μm to 2.3 μm. SEM and FTIR investigations revealed that solvent treatment was effective for removing templates from the resulting solid monolith. The water quality assessments revealed reductions in the turbidity and the total number of suspended particles in the tested wastewater of up to 96–99%. The findings of this study provide insightful knowledge regarding the fabrication of monoliths with homogenous pores that are beneficial for wastewater treatment.

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Recent advances in antibody-based monolith chromatography for therapeutic applications

Cited by 3 publications

References 34 publications

Research of saccharides and related biocomplexes: A review with recent techniques and applications

Research of saccharides and related biocomplexes: A review with recent techniques and applications

Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

Preparation of Polystyrene Microsphere-Templated Porous Monolith for Wastewater Filtration

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