Recent advances in AFM-based biological characterization and applications at multiple levels

Liang, Wenfeng; Guo, Jiang; Yang, Xieliu; Wang, Junhai; Yang, Wenguang; Zhang, Hemin; Liu, Lianqing

doi:10.1039/d0sm01106a

Cited by 35 publications

(12 citation statements)

References 142 publications

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“…Despite their complexities and irregularities, the three-dimensional morphology of biological structures can reveal fine anatomical details. The force curve reveals the relationship between the atomic force and the sample-tip distance; the slope of the force curve directly reflects the elasticity of the samples [ 78 ].…”

Section: Quantitative and Qualitative Evaluationmentioning

confidence: 99%

“…Therefore, the rough sample preparation technique does not damage the fragile samples [ 58 ]. The combination of atomic force microscopy and superresolution optics can provide simultaneous images with super time and spatial resolution in biological studies [ 78 ]. Due to improvements in its performance and function, AFM has played an important role in biological studies [ 78 ].…”

Section: Quantitative and Qualitative Evaluationmentioning

confidence: 99%

See 1 more Smart Citation

Tooth Wear and Tribological Investigations in Dentistry

Wang

Zhu

Chen

et al. 2022

Applied Bionics and Biomechanics

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Dental or tooth wear is a physiological process in the life cycle of teeth. Loss of the occlusal surface may cause excessive tooth wear. Several factors may contribute to tooth wear with different intensities and duration in the oral cavity. The oral cavity is generally compared to a tribological system to determine the various types of wear between teeth and restorative materials and assess the amount of dental wear. However, it is challenging to investigate in vitro and in vivo wear owing to the complexity of tooth wear; thus, a clear correlation between in vitro and in vivo data could not be established. This review is aimed at providing an insight into the etiology of tooth wear and tribological investigations in dentistry.

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Section: Quantitative and Qualitative Evaluationmentioning

confidence: 99%

Section: Quantitative and Qualitative Evaluationmentioning

confidence: 99%

Tooth Wear and Tribological Investigations in Dentistry

Wang

Zhu

Chen

et al. 2022

Applied Bionics and Biomechanics

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“…Nonetheless, AFM remains a great tool for SM studies and has a growing interest and number of applications. For further details on AFM studies, the reader is referred to several independent reviews on AFM-based SM studies [177,[183][184][185].…”

Section: Single-molecule Experimentsmentioning

confidence: 99%

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

Rahman

Islam

et al. 2022

Micromachines

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Single-molecule techniques have shifted the paradigm of biological measurements from ensemble measurements to probing individual molecules and propelled a rapid revolution in related fields. Compared to ensemble measurements of biomolecules, single-molecule techniques provide a breadth of information with a high spatial and temporal resolution at the molecular level. Usually, optical and electrical methods are two commonly employed methods for probing single molecules, and some platforms even offer the integration of these two methods such as optofluidics. The recent spark in technological advancement and the tremendous leap in fabrication techniques, microfluidics, and integrated optofluidics are paving the way toward low cost, chip-scale, portable, and point-of-care diagnostic and single-molecule analysis tools. This review provides the fundamentals and overview of commonly employed single-molecule methods including optical methods, electrical methods, force-based methods, combinatorial integrated methods, etc. In most single-molecule experiments, the ability to manipulate and exercise precise control over individual molecules plays a vital role, which sometimes defines the capabilities and limits of the operation. This review discusses different manipulation techniques including sorting and trapping individual particles. An insight into the control of single molecules is provided that mainly discusses the recent development of electrical control over single molecules. Overall, this review is designed to provide the fundamentals and recent advancements in different single-molecule techniques and their applications, with a special focus on the detection, manipulation, and control of single molecules on chip-scale devices.

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“…AFM has become a powerful technique for probing live biological systems, providing information on diverse parameters such as morphology, surface ultrastructure and nano-mechanical properties (force nanoscopy) at the submolecular level (Liang et al, 2020). Notable advantages of AFM over the widely used EM techniques include expedient and simple sample preparation, live cell imaging under a controlled physical environment and a high signal-to-noise ratio (S/N).…”

Section: Using Afm To Study Pathogens and Their Behaviormentioning

confidence: 99%

AFM-Based Correlative Microscopy Illuminates Human Pathogens

Bhat

Price

Dahms

2021

Front. Cell. Infect. Microbiol.

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Microbes have an arsenal of virulence factors that contribute to their pathogenicity. A number of challenges remain to fully understand disease transmission, fitness landscape, antimicrobial resistance and host heterogeneity. A variety of tools have been used to address diverse aspects of pathogenicity, from molecular host-pathogen interactions to the mechanisms of disease acquisition and transmission. Current gaps in our knowledge include a more direct understanding of host-pathogen interactions, including signaling at interfaces, and direct phenotypic confirmation of pathogenicity. Correlative microscopy has been gaining traction to address the many challenges currently faced in biomedicine, in particular the combination of optical and atomic force microscopy (AFM). AFM, generates high-resolution surface topographical images, and quantifies mechanical properties at the pN scale under physiologically relevant conditions. When combined with optical microscopy, AFM probes pathogen surfaces and their physical and molecular interaction with host cells, while the various modes of optical microscopy view internal cellular responses of the pathogen and host. Here we review the most recent advances in our understanding of pathogens, recent applications of AFM to the field, how correlative AFM-optical microspectroscopy and microscopy have been used to illuminate pathogenicity and how these methods can reach their full potential for studying host-pathogen interactions.

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Recent advances in AFM-based biological characterization and applications at multiple levels

Abstract: Atomic force microscopy (AFM) has found a wide range of bio-applications in the past decades due to its ability to measure biological samples in natural environments at a high spatial...

Cited by 35 publications

References 142 publications

Tooth Wear and Tribological Investigations in Dentistry

Tooth Wear and Tribological Investigations in Dentistry

A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices

AFM-Based Correlative Microscopy Illuminates Human Pathogens

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