Recent advances in 3D hydrogel culture systems for mesenchymal stem cell-based therapy and cell behavior regulation

Hu, Xiaoye; Xia, Zengzilu; Cai, Kaiyong

doi:10.1039/d1tb02537f

Cited by 36 publications

(23 citation statements)

References 258 publications

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“…In 3D cultures, MSCs express HIF1α and VEGF, that favor the establishment of a hypoxic gradient contributing to HSC quiescence, metabolism, and survival [ 39 ]. For example, umbilical cord-derived MSCs cultured in an alginate-gelatin bio-printed hydrogel scaffold show increased long-term viability and stemness maintenance compared to 2D monolayer cultures [ 40 , 41 ]. 3D co-cultures induce proliferation of CD34 + stem cells and increased expression of homing markers, such as C-X-C chemokine receptor type 4 (CXCR-4), the alpha-chemokine receptor for stromal-derived-factor-1 involved in HSC homing to the bone marrow and quiescence [ 21 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

c-Kit M541L variant is related to ineffective hemopoiesis predisposing to clonal evolution in 3D in vitro biomimetic co-culture model of bone marrow niche

Manzo

Scala

Giudice

et al. 2022

Heliyon

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Section: Discussionmentioning

confidence: 99%

c-Kit M541L variant is related to ineffective hemopoiesis predisposing to clonal evolution in 3D in vitro biomimetic co-culture model of bone marrow niche

Manzo

Scala

Giudice

et al. 2022

Heliyon

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“…Notably, hydrogels need to be carefully selected and their inherent properties fine-tuned depending on the intended use. Natural hydrogels including Matrigel, collagen and alginate typically display milder gelling conditions compared to synthetic ones as polyacrylamide or polyethylene glycol ( Hu et al., 2022 ). Indeed, most of 3D cultures have relied on the use of natural hydrogels ( Santos Rosalem et al., 2020 ).…”

Section: Organs-on-a-chipmentioning

confidence: 99%

Advancing Key Gaps in the Knowledge of Plasmodium vivax Cryptic Infections Using Humanized Mouse Models and Organs-on-Chips

Aparici-Herraiz

Caires

Castillo-Fernandez

et al. 2022

Front. Cell. Infect. Microbiol.

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Plasmodium vivax is the most widely distributed human malaria parasite representing 36.3% of disease burden in the South-East Asia region and the most predominant species in the region of the Americas. Recent estimates indicate that 3.3 billion of people are under risk of infection with circa 7 million clinical cases reported each year. This burden is certainly underestimated as the vast majority of chronic infections are asymptomatic. For centuries, it has been widely accepted that the only source of cryptic parasites is the liver dormant stages known as hypnozoites. However, recent evidence indicates that niches outside the liver, in particular in the spleen and the bone marrow, can represent a major source of cryptic chronic erythrocytic infections. The origin of such chronic infections is highly controversial as many key knowledge gaps remain unanswered. Yet, as parasites in these niches seem to be sheltered from immune response and antimalarial drugs, research on this area should be reinforced if elimination of malaria is to be achieved. Due to ethical and technical considerations, working with the liver, bone marrow and spleen from natural infections is very difficult. Recent advances in the development of humanized mouse models and organs-on-a-chip models, offer novel technological frontiers to study human diseases, vaccine validation and drug discovery. Here, we review current data of these frontier technologies in malaria, highlighting major challenges ahead to study P. vivax cryptic niches, which perpetuate transmission and burden.

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“…Combining the properties of each comonomer can generate new properties [21] . The most significant advantage of hydrogels is their compatibility with various materials, which allows them to effectively load nanomaterials, polymers, drugs, biomacromolecules, and even living cells [22][23][24][25] . Moreover, as loading matrixes, hydrogels can retain and enhance the properties of their loaded contents and even eliminate some of their inherent defects.…”

Section: Introductionmentioning

confidence: 99%

Application of optical hydrogels in environmental sensing

Yang

Sarkar

Xie

et al. 2023

Preprint

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The ever-increasing complexity of environmental pollutants urgently warrants the development of new detection technologies. In this context, sensors based on the optical properties of hydrogels enabling fast and easy in situ detection are attracting increasing attention. Herein, the target recognition and sensing mechanisms of two main types of optical hydrogels (OHs) are reviewed and discussed: photonic crystal hydrogels (PCHs) and fluorescent hydrogels (FHs). For PCHs, the environmental stimulus response, target receptors, inverse opal structures, and molecular imprinting techniques related to PCHs are reviewed and summarized. Furthermore, the different types of fluorophores (i.e., compound probes, biomacromolecules, quantum dots, and luminescent microbes) of FHs are summarized. Finally, the data from 138 papers about different OHs are extracted for secondary statistical analysis. The detection performance and potential of various OH types in different environmental pollutant detection scenarios are evaluated, and compared them to those obtained using the standard detection method. Based on this analysis, some possible development directions are proposed, including the fusion of various OHs, introduction of more hydrogel technologies from the biomedical field to the environmental pollutant detection field, and development of multifunctional sensor arrays.

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Recent advances in 3D hydrogel culture systems for mesenchymal stem cell-based therapy and cell behavior regulation

Abstract: Mesenchymal stem cells (MSCs) have been increasingly recognized as resources for disease treatments and regenerative medicine. Meanwhile, the unique chemical and physical properties of hydrogels provide innate advantages to achieve...

Cited by 36 publications

References 258 publications

c-Kit M541L variant is related to ineffective hemopoiesis predisposing to clonal evolution in 3D in vitro biomimetic co-culture model of bone marrow niche

c-Kit M541L variant is related to ineffective hemopoiesis predisposing to clonal evolution in 3D in vitro biomimetic co-culture model of bone marrow niche

Advancing Key Gaps in the Knowledge of Plasmodium vivax Cryptic Infections Using Humanized Mouse Models and Organs-on-Chips

Application of optical hydrogels in environmental sensing

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