Recapitulating Development: A Template for Periodontal Tissue Engineering

Ripamonti, Ugo

doi:10.1089/ten.2006.0167

Cited by 54 publications

(58 citation statements)

References 85 publications

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“…These changes are intriguing, especially because Wnt and TGFβ signaling have both been implicated in cementogenesis [80–84]. However, like the changes in PP i -associated genes, these were not identified until 26 dpn, after the primary cementum defect in Bsp −/− had been evident for some time.…”

Section: Discussionmentioning

confidence: 99%

Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis

Chavez

Chu

et al. 2017

Bone

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Although acellular cementum is essential for tooth attachment, factors directing its development and regeneration remain poorly understood. Inorganic pyrophosphate (PPi), a mineralization inhibitor, is a key regulator of cementum formation: tissue-nonspecific alkaline phosphatase (Alpl/TNAP) null mice (increased PPi) feature deficient cementum, while progressive ankylosis protein (Ank/ANK) null mice (decreased PPi) feature increased cementum. Bone sialoprotein (Bsp/BSP) and osteopontin (Spp1/OPN) are multifunctional extracellular matrix components of cementum proposed to have direct and indirect effects on cell activities and mineralization. Studies on dentoalveolar development of Bsp knockout (Bsp−/−) mice revealed severely reduced acellular cementum, however underlying mechanisms remain unclear. The similarity in defective cementum phenotypes between Bsp−/− mice and Alpl−/− mice (the latter featuring elevated PPi and OPN), prompted us to examine whether BSP is operating by modulating PPi-associated genes. Genetic ablation of Bsp caused a 2-fold increase in circulating PPi, altered mRNA expression of Alpl, Spp1, and Ank, and increased OPN protein in the periodontia. Generation of a Bsp knock-out (KO) cementoblast cell line revealed significantly decreased mineralization capacity, 50% increased PPi in culture media, and increased Spp1 and Ank mRNA expression. While addition of 2 μg/ml recombinant BSP altered Spp1, Ank, and Enpp1 expression in cementoblasts, changes resulting from this dose were not dependent on the integrin-binding RGD motif or by genetic ablation of Ank on the Bsp−/− MAPK/ERK signaling pathway. Decreasing PPi mouse background reestablished cementum formation, allowing more than 3-fold increased acellular cementum volume compared to WT. However, deleting Ank did not fully compensate for the absence of BSP. Bsp−/−;Ank−/− double-deficient mice exhibited mean 20–27% reduced cementum thickness and volume compared to Ank−/− mice. From these data, we conclude that the perturbations in PPi metabolism are not solely driving the cementum pathology in Bsp−/− mice, and that PPi is more potent than BSP as a cementum regulator, as shown by the ability to override loss of BSP by lowering PPi. We propose that BSP and PPi work in concert to direct mineralization in cementum and likely other mineralized tissues.

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Section: Discussionmentioning

confidence: 99%

Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis

Chavez

Chu

et al. 2017

Bone

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“…Taba et al [22] have showed that BMP-2 and -7 stimulate cementogenesis during periodontium development. The incisive work of Ripamonti has shown that BMPs are the soluble signals that initiate regeneration of the periodontal tissues, including the induction of cementogenesis [23].…”

Section: Introductionmentioning

confidence: 99%

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Açil

Yang

Gülses³

et al. 2015

Odontology

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The aim of this study was to assess the factors, mechanisms and the differences between periodontal ligament (PDL) cells and denta l follicle (DF) progenitor cells towards the osteoblastic/cementoblastic differentiation and to investigate the effects of BMP-7 on developmental (DF) and mature tissue-derived (PDL) cells, respectively. Primary cell culture of PDL cells and DF progenitor cells was performed. Osteogenic differentiation was evaluated using von Kossa, Alizarin Red S and immuno-histo-chemistry staining of osteocalcin. Gene expression pattern was evaluated via real-time PCR. A series of CD surface marks were tested using flow cytometry and fluorescence-activated cell-sorting analysis was performed. Real-time RT-PCR demonstrated similar gene expression pattern of PDL cells and DF progenitor cells: the expression of OPN and OCN significantly was elevated when incubated with osteogenic components, Runx2 was unaffected, and Osteorix was hardly expressed whether in basic medium or induction medium. In addition, BMP-7 induced osteoblast/cementoblast differentiation of PDLSCs and DF progenitor cells in a dose- and time-dependent manner, as reflected by enhanced Runx2 and (OCN) mRNA transcript expression. BMP-7 triggers PDL cells and DF progenitor cells to differentiate towards an osteoblast/cementoblast phenotype.

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“…Or how the soluble molecular signals of the transforming growth factor-␤ (TGF-␤) supergene family are deployed so as to initiate de novo bone formation by induction [1][2][3]? Somehow more simply but very confusing in a scenario of redundancy of multiple soluble molecular signals initiating bone differentiation by induction in the primate, which are the molecular signals that initiate de novo bone formation by induction [1][2][3][4][5][6][7][8][9][10]?…”

Section: Introduction: the Induction Of Bone Formation The Emergencementioning

confidence: 99%

“…To be truthful, we have always assigned a prominent and pivotal role to the osteogenic proteins (OPs) of the TGF-␤ supergene family [4,5]. There is no bone formation by induction without the osteogenic soluble molecular signals of the TGF-␤ supergene family [1][2][3][4][5][6][7][8][9][10]. We have, however, always assigned additional prominent critical roles to biomimetic matrices capable of delivering the biological activity of the osteogenic soluble molecular signals [1][2][3][4][5][6][7][8][9][10].…”

Section: Introduction: the Induction Of Bone Formation The Emergencementioning

confidence: 99%

Biomimetism, biomimetic matrices and the induction of bone formation

Ripamonti

2009

J Cellular Molecular Medi

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Bone formation by induction initiates by invocation of osteogenic soluble molecular signals of the transforming growth factor-β (TGF-β) superfamily; when combined with insoluble signals or substrata, the osteogenic soluble signals trigger the ripple-like cascade of cell differentiation into osteoblastic cell lines secreting bone matrix at site of surgical implantation. A most exciting and novel strategy to initiate bone formation by induction is to carve smart self-inducing geometric concavities assembled within biomimetic constructs. The assembly of a series of repetitive concavities within the biomimetic constructs is endowed with the striking prerogative of differentiating osteoblast-like cells attached to the biomimetic matrices initiating the induction of bone formation as a secondary response. Importantly, the induction of bone formation is initiated without the exogenous application of the osteogenic soluble molecular signals of the TGF-β superfamily. This manuscript reviews the available data on this fascinating phenomenon, i.e. biomimetic matrices that arouse and set into motion the mammalian natural ability to heal thus constructing biomimetic matrices that in their own right set into motion inductive regenerative phenomena initiating the cascade of bone differentiation by induction biomimetizing the remodelling cycle of the primate cortico-cancellous bone.

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Recapitulating Development: A Template for Periodontal Tissue Engineering

Cited by 54 publications

References 85 publications

Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis

Overlapping functions of bone sialoprotein and pyrophosphate regulators in directing cementogenesis

Isolation, characterization and investigation of differentiation potential of human periodontal ligament cells and dental follicle progenitor cells and their response to BMP-7 in vitro

Biomimetism, biomimetic matrices and the induction of bone formation

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