Reassessment of antioxidant activity of arbutin: Multifaceted evaluation using five antioxidant assay systems

Takebayashi, Jun; Ishii, Rie; Chen, Jianbin; Matsumoto, Toshio; Ishimi, Yoshiko; Tai, Akihiro

doi:10.3109/10715761003610760

Cited by 73 publications

(55 citation statements)

References 29 publications

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“…The AA-2βG and AA scavenging reactions were rapidly affected at pH 3.0. Such findings are consistent with the results of others demonstrating the capacity of AA-2βG to efficiently scavenge DPPH in vitro (Takebayashi et al, 2008(Takebayashi et al, , 2010. .…”

Section: Dpph Radical Scavenging Activitysupporting

confidence: 95%

“…As a natural AA analogue, AA-2βG has high antioxidant activity and may play pharmacological roles in antioxidant, antiaging, and antitumorigenic activities (Takebayashi et al, 2006(Takebayashi et al, , 2008(Takebayashi et al, , 2010Zhang et al, 2011). However, the AA-2βG antioxidative mechanism remains poorly understood due to a lack of in vivo investigations.…”

Section: Introductionmentioning

confidence: 99%

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Comparative evaluation of the antioxidant effects of the natural vitamin C analog 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from Goji berry fruit

et al. 2011

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2-O-β-D-Glucopyranosyl-L-ascorbic acid (AA-2βG) is a natural derivative of vitamin C (Lascorbic acid, AA) isolated from Goji berry (Lycium barbarum L.) fruit. We evaluated the antioxidant activities of AA-2βG and AA using in vitro and in vivo model systems. In vitro radical scavenging assays demonstrated that AA-βG was capable of scavenging 1,1-diphenyl-2-picryl-hydrazyl and hydroxyl peroxide and inhibiting H(2)O(2)-induced hemolysis better than AA. AA-2βG and AA had similar hydroxyl radical scavenging capabilities, but AA-2βG was incapable of scavenging superoxide anion radicals, and its capacity to scavenge nitrite (NO(2) (-)) was lower than that of AA. The overall in vitro reduction capability of AA-2βG was also significantly lower than that of AA. Moreover, in vivo studies demonstrated that AA-2βG was capable of protecting the liver against carbon tetrachloride-induced acute liver injury in mice. These results suggest that AA-2βG is an important antioxidant component of Goji berry fruit, which may share similar but distinct antioxidant mechanistic properties with AA. This study furthers our understanding of the mechanisms of Goji berry fruit pharmacological activities on antiaging and antitumor properties as a traditional medicine and dietary supplement.

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Section: Dpph Radical Scavenging Activitysupporting

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Comparative evaluation of the antioxidant effects of the natural vitamin C analog 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from Goji berry fruit

et al. 2011

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“…Arbutin has diverse functions, including that it is an anti-oxidant and a depigmenting agent [7]. In erythrocytes, arbutin shows long-lasting radical scavenging properties and also protects a membrane lipid from oxidative stress in human skin fibroblasts [8][9][10]. In cultured human melanoma cells (HMV-II and A375), mouse melanoma cells (B16), and a three-dimensional cultured human skin model, arbutin at concentrations below 300 lg/ ml decreased both tyrosinase activity and melanin content to 40 % of that in the control, thus blocking melanogenesis [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells

Kim

2012

Inflamm. Res.

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“…Recently, we reassessed the antioxidant activity of arbutin using five in vitro assay systems, though arbutin has been reported to possess weak antioxidant activity as compared to its precursor, hydroquinone. 12) We found that arbutin exerted strong antioxidant activity, comparable or even superior to that of hydroquinone in the ABTS þ -scavenging assay, the ORAC assay, and two cell-based antioxidant assays.…”

mentioning

confidence: 99%

Antioxidant Properties of Ethyl Vanillinin Vitroandin Vivo

Tai¹,

Sawano²,

Yazama³

2011

Bioscience, Biotechnology, and Biochemistry

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We systematically evaluated the antioxidant activity of ethyl vanillin, a vanillin analog, as compared with the activities of vanillin and other vanillin analogs using multiple assay systems. Ethyl vanillin and vanillin exerted stronger antioxidant effects than did vanillyl alcohol or vanillic acid in the oxygen radical absorbance capacity (ORAC) assay, although the antioxidant activities of vanillyl alcohol and vanillic acid were clearly superior to those of ethyl vanillin and vanillin in the three model radical assays. The antioxidant activity of ethyl vanillin was much stronger than that of vanillin in the oxidative hemolysis inhibition assay, but was the same as that of vanillin in the ORAC assay. Oral administration of ethyl vanillin to mice increased the concentration of ethyl vanillic acid, and effectively raised antioxidant activity in the plasma as compared to the effect of vanillin. These data suggest that the antioxidant activity of ethyl vanillin might be more beneficial than has been thought in daily health practice.Key words: ethyl vanillin; ABTS radical cation; oxygen radical absorbance capacity (ORAC); oxidative hemolysis inhibition assay; plasma antioxidant activityThe association of reactive oxygen species and free radicals with many disease states is now well recognized, and antioxidants have attracted considerable attention.1,2) It has been reported that there are two types of antioxidants that scavenge radicals quickly and quench many radicals, and it has been proposed that reactivity should be assessed on the basis of both reaction rate and stoichiometry, 3) and that multiple methods should be used, since the activities of some antioxidants vary depending on the assay method.3-6) In addition, comparative studies using common antioxidants appear to be essential to clarify the biological significance of the activities of samples. We have assessed antioxidant activities with attention to the above-mentioned proposals and points of view. We found that 2-O--D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable ascorbic acid derivative, exerted radical-scavenging activity toward unnatural model radicals, including DPPH radical [7][8][9][10] and ABTS radical cation (ABTS þ ). 9,11) The chemical properties of AA-2G as a radical scavenger were widely different from those of ascorbic acid, in that the reaction rate with these model radicals of AA-2G was much slower, but the long-term radical scavenging ability per molecule of AA-2G was superior to that of ascorbic acid. Recently, we reassessed the antioxidant activity of arbutin using five in vitro assay systems, though arbutin has been reported to possess weak antioxidant activity as compared to its precursor, hydroquinone.12) We found that arbutin exerted strong antioxidant activity, comparable or even superior to that of hydroquinone in the ABTS þ -scavenging assay, the ORAC assay, and two cell-based antioxidant assays.Vanillin is widely used in foods, beverages, cosmetics, and drugs. It has been reported to have multifunctional effects, including an...

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Reassessment of antioxidant activity of arbutin: Multifaceted evaluation using five antioxidant assay systems

Cited by 73 publications

References 29 publications

Comparative evaluation of the antioxidant effects of the natural vitamin C analog 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from Goji berry fruit

Comparative evaluation of the antioxidant effects of the natural vitamin C analog 2-O-β-D-glucopyranosyl-L-ascorbic acid isolated from Goji berry fruit

Anti-inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells

Antioxidant Properties of Ethyl Vanillinin Vitroandin Vivo

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