The use of wearable photoplethysmography (PPG) technology for estimating heart rate (HR) and HR variability (HRV) in the health care system is gaining attention in recent years. However, the performance of these devices remains questionable in their ability to collect data in real working conditions for long-term monitoring. The present study aimed to examine the data collected from nurses during working hours by PPG and electrocardiography (ECG) devices. Twenty-two nurses underwent a 60-minute work protocol during the normal working conditions while wearing a PPG device and an ECG device. HR, low-frequency component (LF) and high-frequency component (HF), LF/HF ratio, and percent LF distribution in total spectral power, and steps were examined. Pearson's correlation analysis and Bland-Altman method was performed to examine the relationships between the two devices based on HR and HRV indices. The results found strong positive correlations between HR estimates of both devices, and moderate correlations between LF/HF ratio and percent LF indices estimates, respectively. Moreover, the Bland-Altman analysis showed a small mean bias in general between the captured data of both devices. This pilot study suggested that the PPG device appears to demonstrate good overall reliability in measuring HR, LF/HF ratio, and percent LF. A further large-scale study is required to investigate the feasibility and practicality for HR and HRV analysis in nurses during real working conditions using PPG devices.