Real-Time Zeta Potential Analysis of Microchannel Surfaces during Aminosilane Deposition and Exposure Using Current Monitoring

Abstract

Surface coatings are extensively used in capillary electrophoresis to increase separation efficiency and resolution. The stability of these coatings across a wide pH range is desirable to achieve repeatable migration times; therefore, a comprehensive understanding of coating degradation timescales is needed. We present a novel platform for automated zeta potential analysis based upon current monitoring that delivers improved time resolution over the existing methods. Using our platform, we measure the zeta potential continuously during aminosilane coating reactions and infer changes in the surface composition. We found that the change in the zeta potential after coating depended on the monomer type and solvent, while its stability was influenced by the coating solvent and exposure pH. Our versatile platform provides an elegant approach for evaluating the molecular composition, reactivity, and stability of surfaces in real time.