Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography

K, Kim; Kim, Kyungsang; Park, HyunJoo; Ye, Jong Chul; Park, YongKeun

doi:10.1364/oe.21.032269

Cited by 166 publications

(136 citation statements)

References 59 publications

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“…Employing numerical propagation methods, the 3D tracking of a micrometer-sized particle is enabled using holographic imaging [130]. Via the tomographic reconstruction method, the 3D RI distribution of individual biological samples can be obtained [40,69,131]. Recently, 3D RI measurements demonstrated the quantitative measurement of local RI formation of individual RBCs [68,132].…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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The identification and quantification of specific molecules are crucial for studying the pathophysiology of cells, tissues, and organs as well as diagnosis and treatment of diseases. Recent advances in holographic microspectroscopy, based on quantitative phase imaging or optical coherence tomography techniques, show promise for label-free noninvasive optical detection and quantification of specific molecules in living cells and tissues (e.g., hemoglobin protein). To provide important insight into the potential employment of holographic spectroscopy techniques in biological research and for related practical applications, we review the principles of holographic microspectroscopy techniques and highlight recent studies.

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Section: Discussion and Perspectivesmentioning

confidence: 99%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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“…(2) Even though the particular focus of this study is on static 3-D images of phytoplankton, dynamics of 3-D RI tomograms of individual phytoplankton can allow to measure intracellular dynamics of subcellular organelles [39][40][41] or dynamics fluctuation in cell membrane [42][43][44][45][46][47] which can provide abundant information about pathophysiology of phytoplankton. (3) In addition, recent advances in QPI techniques can also be further employed to investigate phytoplankton research including super-resolution imaging [48], Fourier transform light scattering technique [49][50][51][52][53], real-time visualization of 3-D RI maps [54], multi-spectral QPI [55][56][57][58][59], and polarization-sensitive QPI [60,61]. (4) Furthermore, QPI techniques can be accessible to an existing microscope by attaching the recently developed QPI unit [62,63], which will further extend the applicability of QPI techniques for the study of phytoplankton.…”

Section: Discussionmentioning

confidence: 99%

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

Lee¹,

K²,

Mubarok³

et al. 2014

Journal of the Optical Society of Korea

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Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

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“…Commercially available DMDs have a full-frame display rate up to 32 kHZ. When combined with a high-speed camera, the maximum acquisition rate for 3-D RI tomograms can reach up to 3 kHz because 10 different illumination beams are enough to construct a robust tomograms [42,43].…”

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Active illumination using a digital micromirror device for quantitative phase imaging

et al. 2015

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We present a powerful and cost-effective method for active illumination using a digital micromirror device (DMD) for quantitative phase imaging techniques. Displaying binary illumination patterns on a DMD with appropriate spatial filtering, plane waves with various illumination angles are generated and impinged onto a sample. Complex optical fields of the sample obtained with various incident angles are then measured via Mach-Zehnder interferometry, from which a high-resolution two-dimensional synthetic aperture phase image and a three-dimensional refractive index tomogram of the sample are reconstructed. We demonstrate the fast and stable illumination control capability of the proposed method by imaging colloidal spheres and biological cells, including a human red blood cell and a HeLa cell.Quantitative phase imaging (QPI) has emerged as an invaluable tool for imaging small transparent objects, such as biological cells and tissues [1,2]. QPI employs various interferometric microscopy techniques, including quantitative phase microscopy [2] and digital holographic microscopy [3], to quantitatively measure the optical phase delay of samples. In particular, the measured optical phase delay provides information about the morphological and biochemical properties of biological samples at the single-cell level. Recently, QPI techniques have been widely applied to study the pathophysiology of various biological cells and tissues, including red blood cells (RBCs) [4][5][6][7], white blood cells [8], bacteria [9][10][11], neurons [12][13][14], and cancer cells [15,16].Controlling the illumination beam is crucial in QPI. Especially for measuring 3-D refractive index (RI) tomograms [17] or 2-D highresolution synthetic aperture images [18], angles of plane wave illumination impinging onto a sample should be systematically controlled, and the corresponding light field images of the sample should be measured. Traditionally, galvanometer-based rotating mirrors have been used to control the angle of the illumination beam. A galvanometer-based rotating mirror located at the plane that is conjugate to a sample can control the angle of the incident beam by tilting the mirror with a certain electric voltage.The use of galvanometers, however, has several disadvantages. Inherently, there exists mechanical instability due to position jittering induced by electric noise and positioning error at high voltages. When a two-axis galvanometer is used, the rotational surfaces of each axis cannot be simultaneously conjugate to a sample due to its geometry, and this may induce unwanted additional quadratic phase distribution on the illumination beam that can limit the accurate measurements of 3-D RI tomograms. To solve this optical misalignment, two one-axis galvanometers can be placed at separate conjugate planes, but it requires a bulky optical setup with a long optical path, which can deteriorate phase noise. More importantly, galvanometers cannot generate acomplex wavefront; only tilting of a plane wave is permitted. Recently, a spatial lig...

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Real-time visualization of 3-D dynamic microscopic objects using optical diffraction tomography

Cited by 166 publications

References 59 publications

Biomedical applications of holographic microspectroscopy [Invited]

Biomedical applications of holographic microspectroscopy [Invited]

High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

Active illumination using a digital micromirror device for quantitative phase imaging

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