High-Resolution 3-D Refractive Index Tomography and 2-D Synthetic Aperture Imaging of Live Phytoplankton

Abstract

Optical measurements of the morphological and biochemical imaging of phytoplankton are presented. Employing quantitative phase imaging techniques, 3-D refractive index maps and high-resolution 2-D quantitative phase images of individual live phytoplankton are simultaneously obtained without exogenous labeling agents. In addition, biochemical information of individual phytoplankton including volume, mass, and density of individual phytoplankton are also quantitatively obtained from the measured refractive index distributions. We expect the present method to become a powerful tool for the study of phytoplankton.

Measurements of morphological and biophysical alterations in individual neuron cells associated with early neurotoxic effects in Parkinson's disease

“…We exploit the fact that RI is an intrinsic optical property of materials and linearly proportional to material concentration (26). Recently, measuring 3D RI distributions has been widely applied to study the pathophysiology of various biological samples, such as red blood cells (28)(29)(30)(31)(32), white blood cells (33)(34)(35), cancer cells (36)(37)(38)(39)(40)(41)(42), phytoplankton (43), and bacteria (44)(45)(46). Thus, dry mass of non-aqueous molecules making up the cell can be measured from RI information without any invasive labeling process.…”

3D label-free imaging and analysis of Pinus pollen grains using optical diffraction tomography

“…Reconstruction of the RI maps was conducted using the principle previously proposed (Wolf, 1969). Detailed descriptions of the reconstruction algorithm can be found elsewhere (Kim, K et al, 2014;Lim et al, 2015).…”

“…More recently, 3D QPI techniques including optical diffraction tomography (ODT) have been extensively utilized for imaging and analyzing the internal 3D structures of various transparent biological samples, including yeast cells (Habaza et al, 2015), mammalian blood cells (Kim, K et al, 2014;Hur et al, 2017;Merola et al, 2017;Yoon et al, 2017), mammalian eukaryotic cells (Kim, T et al, 2014;Kim et al, 2016;Yang et al, 2017), and microorganisms (Cotte et al, 2013;Lee et al, 2014). ODT is a technique that reconstructs a 3D RI map from light scattered from a sample subjected to illumination at different angles (Wolf, 1969).…”

Tomographic phase microscopy: principles and applications in bioimaging [Invited]

“…However, the underlying mechanisms of growth, movement, and biosynthesis of LDs have not been well understood. The fact that the RI values of LDs are distinctly higher than that of the cytoplasm offers TPM an opportunity to segment out LDs and act as a 3D label-free tool for their imaging and quantification [31,55,112]. Using TPM, Kim et al [55] obtained the time lapse of the 3D RI distributions of LDs inside a hepatocyte cell.…”