Real-time tracking and quantification of endogenous hydrogen peroxide production in living cells using graphenated carbon nanotubes supported Prussian blue cubes

Balamurugan, T.S.T.; Mani, Veerappan; Hsieh, Chang-Che; Huang, Sheng‐Tung; Peng, Tie-Kun; Lin, Hsin-Yi

doi:10.1016/j.snb.2017.10.151

Cited by 48 publications

(19 citation statements)

References 56 publications

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“…fMLP stimulates the generation of H 2 O 2 by cells. [ 14,42 ] The as‐fabricated H 2 O 2 sensor was used to detect H 2 O 2 released from mouse tumor cell lines. In this study, 4T1, LLC and CT26 cell lines were selected as cancer models, while the LO2 cell line was used as the control.…”

Section: Resultsmentioning

confidence: 99%

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

Peng

Pan

et al. 2021

Small Methods

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The diagnosis of malignant tumors is essential for informing clinical decisions regarding therapeutic options. Current imaging and pathological diagnostic methods do not provide quantitative molecular information that is important in tumor identification. Moreover, pathological tissue analysis is dependent on unevenly distributed pathological features. The tumor microenvironment has been documented to have hydrogen peroxide (H2O2). This study presents a biologically sensitive and efficient H2O2 electrochemical sensor based on PtNi nanoparticle‐doped N‐reduced graphene oxide (PtNi‐N‐rGO) with a low detection limit (2.8 × 10–9 m), a fast response time (<6 s) and desirable anti‐interference characteristics. Herein, H2O2 is used as molecular biomarker. The sensor successfully captures H2O2 from cancer cells. In addition, it efficiently detects tumor tissues, adjacent tissues, and normal tissues. This study demonstrates the H2O2 sensor potential to rapidly detect tumor tissues. This technique provides a complementary method for pathological tumor diagnosis that is independent of the traditional pathology labs.

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Section: Resultsmentioning

confidence: 99%

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

Peng

Pan

et al. 2021

Small Methods

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“…The described sensor showed detection limit in the nanomolar range and showed excellent selectivity in a complex environment such as the culture medium used, allowing the selective determination of very low amounts of H 2 O 2 without interferences. In addition, in this work H 2 O 2 was quantified instead of following its almost instantaneous release from cells challenged to a stressor in contrast to [45] and [46]. These results could pave the way for a better understanding the neurotoxic effect of ROS using an in vitro model of Parkinson's disease.…”

Section: Discussionmentioning

confidence: 99%

Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson’s disease in vitro model

Rojas

Pelle

Carlo

et al. 2018

Sensors and Actuators B: Chemical

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In this work, we present the combination of Carbon Black (CB) and electrodeposited Prussian Blue (PB) covered with a Nafion layer on Screen-Printed electrodes (CB/PB-SPE) used for non-enzymatic H 2 O 2 sensing in Neuroblastoma cell line SH-SY5Y. These cells were challenged with 6hidroxidopamine (6-OHDA) for modelling Parkinson's disease. The electrodes surface was investigated using Scanning Electron Microscopy (SEM) and electrochemically characterized, in terms of electroactivity and stability. Electrochemical sensing of H 2 O 2 was carried out at very low potentials (-50mV), with a LOD of 0.01 μM and linear range between 0.2 and 1000 μM, allowing interference-free detection of H 2 O 2 in the selected cell culture. The H 2 O 2 concentration was successfully monitored in an experimental model of Parkinson's disease. These results pave the way to a method for the continuous monitoring of H 2 O 2 in culture medium for future studies of the role of H 2 O 2 in Parkinson's disease.

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“…The amount of H 2 O 2 released from each cell was calculated to be 8.7 × 10 −14 mol, which is in good agreement with previous reports. 9,67 The amount of H 2 O 2 released by RAW 264.7 cells was also quantified by a standard fluorimetric H 2 O 2 assay kit. An excitation wavelength (λ ex ) of 640 nm and an emission wavelength (λ em ) of 680 nm were used, and the kit's standard procedure was adopted.…”

Section: Methodsmentioning

confidence: 99%

ZnCo₂O₄ Nanoflowers Grown on Co₃O₄ Nanowire-Decorated Cu Foams for in Situ Profiling of H₂O₂ in Live Cells and Biological Media

Mani

Selvaraj

Peng

et al. 2019

ACS Appl. Nano Mater.

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A robust real-time quantification method is essential to understand the physiological roles of endogenous H2O2 in biological systems. For this purpose, we described a binary transition-metal oxide (TMO)-based nanointerface, i.e., spinal zinc cobaltite/cobalt oxide ternary nanoarrays (ZnCo2O4/Co3O4) on a Cu foam (CF). The ZnCo2O4/Co3O4/CF facilitates H2O2 reduction at a minimized overpotential (−0.10 V vs Ag/AgCl). which is several millivolts away from the voltammetric regions of common biological and oxygen interferences, making the electrode highly selective in the presence of 5-fold excess concentrations of biological species. In the presence of ZnCo2O4, the electrocatalytic capability of Co3O4 has increased significantly by enlarging the electrochemical active area of the electrode (0.538 cm2). A substantial improvement in the stability (97.24%) and reproducibility (relative standard deviation = 3.14%) are attained because the direct growth of nanomaterials is generated on CF in close proximity with the electrode surface and strengthens the affinity. The modified electrode endows ultrasensitivity (detection limit = 1 nM) and quantifies the amount of H2O2 released from mammalian cells (8.7 × 10–14 mol). Binary TMOs hold promise in tailoring a reliable H2O2-detecting interface for real-time, in vivo applications.

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Real-time tracking and quantification of endogenous hydrogen peroxide production in living cells using graphenated carbon nanotubes supported Prussian blue cubes

Cited by 48 publications

References 56 publications

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson’s disease in vitro model

ZnCo₂O₄ Nanoflowers Grown on Co₃O₄ Nanowire-Decorated Cu Foams for in Situ Profiling of H₂O₂ in Live Cells and Biological Media

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Real-time tracking and quantification of endogenous hydrogen peroxide production in living cells using graphenated carbon nanotubes supported Prussian blue cubes

Cited by 48 publications

References 56 publications

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

A Nonenzymatic Hydrogen Peroxide Electrochemical Sensing and Application in Cancer Diagnosis

Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson’s disease in vitro model

ZnCo2O4 Nanoflowers Grown on Co3O4 Nanowire-Decorated Cu Foams for in Situ Profiling of H2O2 in Live Cells and Biological Media

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ZnCo₂O₄ Nanoflowers Grown on Co₃O₄ Nanowire-Decorated Cu Foams for in Situ Profiling of H₂O₂ in Live Cells and Biological Media