Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

Xu, Shicai; Zhan, Jian; Man, Baoyuan; Jiang, Shouzhen; Yue, Weiwei; Gao, Shoubao; Guo, Chaowan; Liu, Hanping; Li, Zhenhua; Wang, Jihua; Zhou, Yaoqi

doi:10.1038/ncomms14902

Cited by 331 publications

(364 citation statements)

References 61 publications

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“…The increased ssDNA capture probe capacity can extend the detection limit and also dynamic range. 23,24 In addition, the micropillar array increases the interaction between biomolecules and the substrate, as the surface area in contact with any given solution is significantly higher. To further increase the probe binding capacity, we can increase the aspect ratio of PDMS micropillars and the microchannel length.…”

Section: Discussionmentioning

confidence: 99%

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Bao

et al. 2020

Preprint

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A fully Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) system is developed for viral DNA detection. This powerful system is patterned with high-aspect ratio micropillars to enhance reporter probe binding. After surface modification and probe immobilization, CRISPR Cas12a/crRNA complex is injected into the fully enclosed system. With the presence of double-stranded DNA target, the CRISPR enzyme is activated and non-specifically cleaves the ssDNA reporters initially immobilized on the micropillars. This collateral cleavage releases fluorescence dyes into the assay, and the intensity is linearly proportional to the target DNA concentration ranging from 0.1 to 10 nM. Importantly, this system does not rely on traditional dye-quencher labeled probe thus eliminating the fluorescence background presented in the assay. Furthermore, our one-step detection protocol is performed at isothermal conditions (37°C) without using complicated and time-consuming off-chip probe hybridization and denaturation. This miniaturized and fully packed IMPACT chip demonstrates rapid, sensitive, and simple nucleic acid detection and is an ideal candidate for the next generation molecular diagnostic platform for point-of-care (POC) applications, responding to emerging and deadly pathogen outbreaks.

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Section: Discussionmentioning

confidence: 99%

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Bao

et al. 2020

Preprint

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“…The change of electric potential of graphene can be explained by the negative electrostatic gating effect. As DNA has a negatively charged triphosphate group, it can modulate the Fermi level of graphene by inducing excess hole carriers and in turn shift cnp to the positive direction [24,25]. Figure 4(a) shows the transfer characteristics of DNA probe-modified G-FETs before and after addition of target RNAs (full-complementary) with different concentration from 0.1 fM to 1 pM.…”

Section: Resultsmentioning

confidence: 99%

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

Tian

Zhang

et al. 2018

Advances in Condensed Matter Physics

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Graphene has attracted much attention in biosensing applications due to its unique properties. In this paper, the monolayer graphene was grown by chemical vapor deposition (CVD) method. Using the graphene as the electric channel, we have fabricated a graphene field-effect transistor (G-FET) biosensor that can be used for label-free detection of RNA. Compared with conventional method, the G-FET RNA biosensor can be run in low cost, be time-saving, and be miniaturized for RNA measurement. The sensors show high performance and achieve the RNA detection sensitivity as low as 0.1 fM, which is two orders of magnitude lower than the previously reports. Moreover, the G-FET biosensor can readily distinguish target RNA from noncomplementary RNA, showing high selectivity for RNA detection. The developed G-FET RNA biosensor with high sensitivity, fast analysis speed, and simple operation may provide a new feasible direction for RNA research and biosensing.

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“…Nanomaterial such as carbon nanotubes and graphene nanosheets are usually added into chitosan as enhancement materials . It is reported that the graphene nanosheets can improve the uniformity and evenness of chitosan thin film and promote DNA hybridization reaction.…”

Section: Recent Developmentsmentioning

confidence: 99%

Recent development in chitosan nanocomposites for surface‐based biosensor applications

Jiang

Cheng

2019

Electrophoresis

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Recent years have witnessed ever expanding use of biosensors in the fields of environmental monitoring, homeland security, pharmaceutical, food and bioprocessing, and agricultural industries. To produce effective and reliable biosensors, good quality immobilization of biological recognition elements is critical. Chitosan and its nanocomposites emerge as an excellent immobilization matrix on biosensor surface. As a natural polysaccharide, chitosan has many useful characteristics, such as high permeability and mechanical strength, biocompatibility and non‐toxicity, availability, and low cost. Due to the presence of amino and hydroxyl groups on chitosan, chitosan can easily crosslink with a variety of nanomaterials. This investigation of chitosan nanocomposite‐based biosensors presents recent development and innovations in the preparation of chitosan nanocomposites in coordination with biosensors for various bio‐detection applications, including chitosan nanocomposites formed with carbon nanomaterials, various inorganic and biological complexes. These chitosan nanocomposite based biosensors have demonstrated good sensitivity selectivity and stability for the detection of different types of targets ranging from glucose, proteins, DNAs, small biomolecules to bacteria. It is in our hope that this review will offer guidance for the development of novel biosensors and open up opportunities in the field of biosensor research.

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Real-time reliable determination of binding kinetics of DNA hybridization using a multi-channel graphene biosensor

Cited by 331 publications

References 61 publications

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

Integrated Micropillar Polydimethylsiloxane Accurate CRISPR Detection (IMPACT) System for Rapid Viral DNA Sensing

RNA Detection Based on Graphene Field-Effect Transistor Biosensor

Recent development in chitosan nanocomposites for surface‐based biosensor applications

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