Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs

Guo, Xiulan; Xia, X. Y.; Tang, Ruimin; Wang, Kangning

doi:10.1016/j.anaerobe.2008.04.001

Cited by 79 publications

(68 citation statements)

References 16 publications

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“…We also demonstrated a linear relationship between the percentage of Bacteroidetes and backfat thickness. These results and backfat thickness data were in accordance with the theory that percentage body fat correlates positively with the abundance of Firmicutes in the gut microbiota in humans and mice (Ley et al, 2005Turnbaugh et al, 2006) and swine (Guo et al, 2008a). This may be because the B. subtilis used in this study belongs to the Firmicutes group, and thus, adding B. subtilis resulted in substantial proliferation of Firmicutes in the pig intestine.…”

Section: Discussionsupporting

confidence: 77%

“…The obese mice ob/ob have a 50% reduction in the abundance of Bacteroidetes and a proportional increase in Firmicutes compared to their lean (+/+) littermates (Ley et al, 2005). The abundance of Bacteroidetes is decreased in obese pigs (Guo et al, 2008a) compared to lean pigs, and the proportion of Bacteroidetes increases with weight loss . These changes in gut microbial communities are division-wide and not attributable to differences in food consumption or dietary calorie content (Ley et al, 2005.…”

Section: Introductionmentioning

confidence: 92%

“…Our team was the first to report on the real-time polymerase chain reaction (PCR) quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs (Guo et al, 2008a). Our previous results demonstrated that body fat was closely related to the percentages of Bacteroidetes division and Bacteroides spp in the gut microbiota of common pig breeds, and that differences in gut microbial ecology in obese versus lean animals could be analogous, although the trend for Firmicutes percentage was not significant.…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have reported that percentage body fat correlates with the abundance of Bacteroidetes and Firmicutes in the gut microbiota in humans, mice (Ley et al, 2005Turnbaugh et al, 2006) and swine (Guo et al, 2008a). The obese mice ob/ob have a 50% reduction in the abundance of Bacteroidetes and a proportional increase in Firmicutes compared to their lean (+/+) littermates (Ley et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism

Cui¹,

Shen²,

GaoWang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The ratio of Bacteroidetes and Firmicutes bacterial groups in the gut can affect the ability to absorb nutrients. We investigated the effect of probiotic Bacillus subtilis supplementation of diets on growth performance, fat deposition, blood lipids, copy numbers, and percentage of Bacteroidetes and Firmicutes in cecal contents, as well as mRNA expression of key lipid metabolism enzymes in the liver and adipose tissue of finishing pigs. Twenty-four Duroc x Meishan crossbreed 8-week-old pigs (10.28 ± 0.59 kg) were randomly allocated to two dietary treatments: maize-soybean mealbased diets with B. subtilis (probiotic group) and without B. subtilis (control group). The probiotic diet led to a significant increase in the average daily gain and feed conversion ratio of pigs weighing 10 to 110 kg. The mean backfat depth was increased while leaf lard weights were decreased by probiotic supplementation. Ingestion of probiotics decreased the serum triglyceride and glucose concentrations, but did not change the levels of total cholesterol and free fatty acids in the serum. The mRNA expressions of fatty acid synthase (FAS) and acetylCoA carboxylase α (ACCα) in the liver were down-regulated by the dietary probiotic supplement. Conversely, the gene expressions of FAS and ACCα in the adipose tissue increased. The probiotic diet decreased the copy numbers and percentage of Bacteroidetes, while it increased the percentage of Firmicutes in the cecal contents. We conclude that the addition of B. subtilis improves growth performance and up-regulates lipid metabolism in subcutaneous fat of finishing pigs. We conclude that B. subtilis affects lipid metabolism through regulation of the proportion of Bacteroidetes and Firmicutes in the gut.

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Section: Discussionsupporting

confidence: 77%

Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism

Cui¹,

Shen²,

GaoWang³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…The loads of total bacteria were quantified by realtime PCR using the 16S RNA gene-specific primers (Guo et al, 2008) and normalized by real-time PCR data for the host β-actin gene according to the method as described previously (Petnicki-Ocwieja et al, 2009). Primer pairs used in quantitative PCR analysis are as follows: 16 S rDNA gene primers (F 5′-ACTCCTACGGGAGGCAGCAG-3′ and R 5′-AT TACCGCGGCTGCTGG-3′) and β-actin (F 5′-TCGATC ATGAAGTGCGATGT-3′ and R 5′-ATCAGCAATAC CGGGGTACA-3′).…”

Section: Real-time Quantitative Pcr Analysismentioning

confidence: 99%

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

Yao¹,

Wang²,

Li³

et al. 2015

The ISME Journal

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The guts of metazoans are in permanent contact with the microbial realm that includes beneficial symbionts, nonsymbionts, food-borne microbes and life-threatening pathogens. However, little is known concerning how host immunity affects gut bacterial community. Here, we analyze the role of a dual oxidase gene (BdDuox) in regulating the intestinal bacterial community homeostasis of the oriental fruit fly Bactrocera dorsalis. The results showed that knockdown of BdDuox led to an increased bacterial load, and to a decrease in the relative abundance of Enterobacteriaceae and Leuconostocaceae bacterial symbionts in the gut. The resulting dysbiosis, in turn, stimulates an immune response by activating BdDuox and promoting reactive oxygen species (ROS) production that regulates the composition and structure of the gut bacterial community to normal status by repressing the overgrowth of minor pathobionts. Our results suggest that BdDuox plays a pivotal role in regulating the homeostasis of the gut bacterial community in B. dorsalis.

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Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler chickens

Zhao

Guo

et al. 2013

Appl Microbiol Biotechnol

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To investigate the effects of Clostridium butyricum and Enterococcus faecium on the growth performance, lipid metabolism, and cecal microbiota of broilers, 264 one-day-old male Ross 308 broiler chicks were randomly allocated into four treatments with six replicates in a 2 × 2 factorial arrangement and fed four diets with two levels of C. butyricum (0 or 1 × 10⁹ cfu/kg) and two levels of E. faecium (0 or 2 × 10⁹ cfu/kg) for 42 days. There was no significant interaction between C. butyricum and E. faecium on the growth performance, lipid metabolism, and cecal microbiota of broilers. However, broilers supplemented with E. faecium had lower (P = 0.022) serum leptin level at day 21 and higher (P < 0.001) fatty acid synthase (FAS), malic enzyme (ME), and acetyl-CoA carboxylase (ACC) mRNA levels in the liver at day 42. Supplementation of C. butyricum improved (P < 0.05) the average daily feed intake and average daily gain, increased (P = 0.016) the serum insulin level at 21 days of age, enhanced (P < 0.05) the content of intramuscular fat, activities of FAS in the liver and lipoprotein lipase (LPL) in the breast muscle, mRNA expression of FAS, ME, and ACC in the liver and LPL in the breast muscle at 42 days of age, but reduced (P = 0.030) cecal Bacteroidetes relative abundance at 21 days of age. The results of this study indicate that the increased intramuscular fat content of broilers fed C. butyricum as observed may be the result of enhanced lipogenesis.

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Real-time PCR quantification of the predominant bacterial divisions in the distal gut of Meishan and Landrace pigs

Cited by 79 publications

References 16 publications

Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism

Effect of dietary Bacillus subtilis on proportion of Bacteroidetes and Firmicutes in swine intestine and lipid metabolism

The dual oxidase gene BdDuox regulates the intestinal bacterial community homeostasis of Bactrocera dorsalis

Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler chickens

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