Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler chickens

Zhao, Xu; Guo, Yuming; Guo, Shuangshuang; Tan, Jianzhuang

doi:10.1007/s00253-013-4970-2

Cited by 92 publications

(74 citation statements)

References 58 publications

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“…PPAR-γ is expressed at the highest level in adipose tissue and promotes adipocyte differentiation (Mandrup and Lane, 1997), and its expression did not change after adding FCSM in the broiler chickens' diet. LPL, a critical enzyme in the hydrolysis of TG, catalyzes TG from circulating chylomicrons and very-low-density lipoprotein in adipose tissue (Zhao et al, 2013). In the present study, the down-regulated mRNA level of LPL in abdominal fat was associated with a decreased fatty acid synthesis, hepatic TG content, and fat deposition in abdominal fat in the chickens fed the diet containing FCSM.…”

Section: Discussionsupporting

confidence: 55%

Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens

Nie

Zhang

Wang

et al. 2015

J. Zhejiang Univ. Sci. B

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This study investigated the effects of fermented cottonseed meal (FCSM) on lipid metabolites, lipid metabolism-related gene expression in liver tissues and abdominal adipose tissues, and hepatic metabolomic profiling in broiler chickens. One hundred and eighty 21-d-old broiler chickens were randomly divided into three diet groups with six replicates of 10 birds in each group. The three diets consisted of a control diet supplemented with unfermented cottonseed meal, an experimental diet of cottonseed meal fermented by Candida tropicalis, and a second experimental diet of cottonseed meal fermented by C. tropicalis plus Saccharomyces cerevisae. The results showed that FCSM intake significantly decreased the levels of abdominal fat and hepatic triglycerides (P<0.05 for both). Dietary FCSM supplementation down-regulated the mRNA expression of fatty acid synthase and acetyl CoA carboxylase in liver tissues and the lipoprotein lipase expression in abdominal fat tissues (P<0.05 for both). FCSM intake resulted in significant metabolic changes of multiple pathways in the liver involving the tricarboxylic acid cycle, synthesis of fatty acids, and the metabolism of glycerolipid and amino acids. These findings indicated that FCSM regulated lipid metabolism by increasing or decreasing the expression of the lipid-related gene and by altering multiple endogenous metabolites. Lipid metabolism regulation is a complex process, this discovery provided new essential information about the effects of FCSM diets in broiler chickens and demonstrated the great potential of nutrimetabolomics in researching complex nutrients added to animal diets.

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Section: Discussionsupporting

confidence: 55%

Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens

Nie

Zhang

Wang

et al. 2015

J. Zhejiang Univ. Sci. B

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“…Barter et al (2010) reported that a link may exist between the changes in HDL-C and TG, but they found no apparent relationship between LDL-C reduction and HDL-C increase. However, Zhang et al (2011) and Zhao et al (2013) observed that diet containing Clostridium butyricum and Enterococcus faecium exerted no effect on the concentrations of TG, TC, HDL-C, and LDL-C in broiler sera. Meanwhile, the serum INS levels were enhanced by C .…”

Section: Discussionmentioning

confidence: 98%

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

et al. 2017

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Numerous studies have focused on the beneficial effects of probiotics in animals. Even so, additional information should be obtained about the mechanisms by which a useful probiotic strain successfully exerts such beneficial effects. In this study, we evaluated the effect of the dietary supplementation of both live and disrupted Lactobacillus johnsonii (LJ) strain BS15 in broilers at different ages. Specifically, growth performance, lipid metabolism, gut microbiota, intestinal development, and digestive ability of the broilers were assessed. A total of 180 1-day-old Cobb 500 chicks were randomly distributed into three groups. These chicks were fed diets supplemented with 1 × 106 colony-forming units (cfu) LJ per gram of feed (LJ group); 1 × 106 cfu disrupted LJ per gram of feed (D-LJ group); and de Man, Rogosa, and Sharpe liquid medium (control group), respectively, throughout a 42-day experimental period. The results demonstrated that LJ supplementation of feed had a positive effect on the average daily gain and starter feed conversion ratio. In addition, LJ supplementation of feed decreased serum triglyceride and low-density lipid cholesterol levels, as well as abdominal fat deposition. LJ also reduced the mRNA levels of lipoprotein lipase in adipose tissue and stearoyl-CoA desaturase-1 in the liver. LJ diminished the mRNA quantities of the sterol regulatory element binding protein-1c and fatty acid synthase, as well as increased the level of serum high-density lipid cholesterol. LJ increased the mRNA quantities of peroxisome proliferator-activated receptor α, acyl-CoA oxidase in the liver, and carnitine palmitoyltransferase-1. LJ also improved the intestinal development and digestive ability mainly by increasing the villus height/crypt depth ratio in the ileum. The probiotic increased the levels of epidermal growth factor and insulin-like growth factor-1, as well as the activities of trypsin and lipase in the jejunum and ileum. LJ exerted beneficial effects on the intestinal flora. Specifically, LJ markedly enhanced the population of Bacteroidetes and Lactobacillus spp. Moreover, the probiotic reduced the population of Enterobacteriaceae and the Firmicutes/Bacteroidetes ratio. Slight changes caused by disrupted LJ were detected. These findings indicated that live LJ supplementation may promote growth performance and lower fat deposition in broilers.

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“…PPAR-α, as a member of nuclear receptors, is highly expressed in the liver and plays an important role in regulating glucose and lipid metabolism or enhancing the related-gene expression in fatty acid oxidation (Lee et al, 1995;Zhang et al, 2011;Huang et al, 2013). LPL, on the other hand, catalyzes triglycerides from circulating chylomicron and very low-density lipoprotein to generate fatty acids and glycerol for tissue utilization or storage (Zhao et al, 2013). Again, this time due to the different changes in the PPAR-α and LPL expression levels in the liver tissue, there was a distinction between CSM fermented by C. tropicalis and CSM fermented by C. tropicalis plus S. cerevisiae.…”

Section: Effect Of Cottonseed Meal Fermented With Yeast On the Lipid-mentioning

confidence: 99%

“…Moreover, Zhao et al (2013) reported that probiotics (Clostridium butyricum and Enterococcus faecium) enhance the mRNA abundance of lipogenic genes in male broilers, including ACC, FAS, and malic enzyme in the liver tissue. However, in the present study, the expressions of FAS and L-FABP in the liver tissue were not altered and, therefore, do not agree with the results of Zhao et al (2013). These differences may be attributed to differences in yeast strain, broiler breed, and feeding system.…”

Section: Effect Of Cottonseed Meal Fermented With Yeast On the Lipid-mentioning

confidence: 99%

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

Nie

et al. 2015

Rev. Bras. Cienc. Avic.

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Fermented cottonseed meal (FCSM) is widely used in poultry diets in China. This study was conducted to investigate the effect of FCSM on lipid-related gene expression in broilers. Initially, 180 broiler chickens (21-days-old, equal number of males and females) were randomly divided into three groups, with six pens per group and 10 birds per pen. The chickens in the control group were fed a diet containing unfermented cottonseed meal, and those in the treatment groups were fed with diets including either CSM fermented by Candida tropicalis (Ct group) or CSM fermented by Candida tropicalis plus Saccharomyces cerevisae (Ct-Sc group) until 64 days old. The results revealed that, compared with the control group (p<0.05), the mRNA expression of peroxisome proliferator-activated receptor alpha (PPAR-α) and lipoprotein lipase (LPL) were upregulated in the livers of Ct-Sc males. The expression of PPAR-α was also upregulated in the livers of Ct females. The expression levels of acetyl CoA carboxylase (ACC) and LPL in the liver of males and the expression of PPAR-α in the liver of females were significantly different between the Ct and Ct-Sc groups (p<0.05). However, gene expressions of fatty acid synthase (FAS) and liver fatty acid-binding protein (L-FABP) in the liver were not altered when the broilers were fed FCSM-supplemented diets (p>0.05). Likewise, the expressions of peroxisome proliferator-activated receptor gamma (PPAR-γ) and LPL in the abdominal fat were not altered by the FCSMsupplemented diets (p>0.05). The results in this study indicate that CSM fermented by Candida tropicalis and Saccharomyces cerevisiae effectively regulated the genes involved in fatty acid β-oxidation and triglyceride hydrolysis in male broiler chickens. Furthermore, the effects of the FCSM-supplemented diets were significantly different between bird sexes and between yeast strains used in the fermentation process.

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Effects of Clostridium butyricum and Enterococcus faecium on growth performance, lipid metabolism, and cecal microbiota of broiler chickens

Cited by 92 publications

References 58 publications

Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens

Tissue lipid metabolism and hepatic metabolomic profiling in response to supplementation of fermented cottonseed meal in the diets of broiler chickens

Live Probiotic Lactobacillus johnsonii BS15 Promotes Growth Performance and Lowers Fat Deposition by Improving Lipid Metabolism, Intestinal Development, and Gut Microflora in Broilers

Effect of Cottonseed Meal Fermented with Yeast on the Lipid-related Gene Expression in Broiler Chickens

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