Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia

Abstract

a b s t r a c tThe present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia.

Qualitative PCR-based detection of genetically modified soy and maize products in Iran

“…[20] However, the purity of obtained DNA can be significantly affected by degree of processing, extraction procedure and different components of sample matrix. [1] Therefore, to reduce the false negative results quality confirmation of genomic DNA for all samples and CRMs were carried out by agarose gel electrophoresis. [21,22] It was observed that the specific lectin and invertase endogenous genes are available for further investigation of GM sequences from soy and maize, respectively (Figure 2).…”

“…[13] The observation of maize food products was consistent with the study conducted in Malaysia which have indicated that of all 20 non-labeled maize samples tested, 13 (65%) were determined to be positive for GM materials. [1] GMO screening study in Kingdom of Saudi Arabia indicated that out of 64 maize containing products, 28 were positive for CaMV 35 S or NOS terminator. [27] In the other study, Gürakan and Aydın (2011), examined 31 maize sold samples for GMO detection by targeting screening materials.…”

“…Gene modification techniques have been applied to a number of agricultural crops in order to insert new appropriate traits such as herbicide tolerance, insect and disease resistance, improve the nutritional content of food and feed products and increasing of various desirable traits. [1,2] Among the genetically modified (GM) plants soybean and maize are the two main cultivated GM crops in the globe, which covered 47% and 32% of the transgenic plants area respectively, and commonly represent as ingredients in many foods. [3,4] About 12 percent of global farming area were planted with these products according to the reports of 2014.…”

PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh

“…However, consumers and environmental groups, have questioned whether the extent of testing is sufficient [3]. The controversy surrounding GMO crops has left consumers uncertain about the issue [4]. Fears for the safety of the consumption of foods made from GMO crops are also due that at the beginning the preparation of transgenic plants involves the bacterium Agrobacterium tumefacient using the Ti plasmid as a gene transfer vector.…”

Detection of genetically modified DNA in fresh and processed foods sold in Kuwait

“…Currently, a number of countries are actively pursuing detection and monitoring of GM products in food commodities. Some examples of those studies performed in various countries include reports from Malaysia, 24,25 Brazil, 26 China, 27 Saudi Arabia, 28 etc.…”