Real-Time Label-Free Surface Plasmon Resonance  Biosensing with Gold Nanohole Arrays Fabricated by Nanoimprint Lithography

Martínez-Perdiguero, Josu; Retolaza, A.; Otaduy, D.; Juarros, A.; Merino, S.

doi:10.3390/s131013960

Cited by 27 publications

(11 citation statements)

References 16 publications

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“…In this sensor, adsorption of the analyte to the array surface results in a shift in the wavelengths transmitted through the “nanohole” array. 161 The fabrication of these arrays followed the standard NIL imprinting process (with a silicon stamp) of a thermoplastic resist coated on glass wafers ( Figure 2 G). After the imprinting and etching of the residual resist layer, a 50 nm gold layer is deposited followed by the resist lift-off, resulting in a grid of 185-nm-diameter nanoholes with a periodicity of 450 nm.…”

Section: Applications Of Large-scale Lithographic Technologiesmentioning

confidence: 99%

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

2021

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Since the early 2000s, extensive research has been performed to address numerous challenges in biochip and biosensor fabrication in order to use them for various biomedical applications. These biochips and biosensor devices either integrate biological elements (e.g., DNA, proteins or cells) in the fabrication processes or experience post fabrication of biofunctionalization for different downstream applications, including sensing, diagnostics, drug screening, and therapy. Scalable lithographic techniques that are well established in the semiconductor industry are now being harnessed for large-scale production of such devices, with additional development to meet the demand of precise deposition of various biological elements on device substrates with retained biological activities and precisely specified topography. In this review, the lithographic methods that are capable of large-scale and mass fabrication of biochips and biosensors will be discussed. In particular, those allowing patterning of large areas from 10 cm 2 to m 2 , maintaining cost effectiveness, high throughput (>100 cm 2 h –1 ), high resolution (from micrometer down to nanometer scale), accuracy, and reproducibility. This review will compare various fabrication technologies and comment on their resolution limit and throughput, and how they can be related to the device performance, including sensitivity, detection limit, reproducibility, and robustness.

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Section: Applications Of Large-scale Lithographic Technologiesmentioning

confidence: 99%

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

2021

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“…The period in x and y directions is P = 500 nm. The selected values for these parameters were chosen according to previous studies for obtaining high sensitivity to changes in the buffer refractive index in the visible spectral region [43,44]. The nanoarray is located on a glass substrate.…”

Section: Theoretical Methodsmentioning

confidence: 99%

Electromagnetic behavior of dielectric objects on metallic periodically nanostructured substrates

Barreda¹,

Otaduy²,

Martín-Rodríguez³

et al. 2018

Opt. Express

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In this research, we investigate the electromagnetic behavior of a metallic thin-film with a periodic array of subwavelength apertures when dielectric objects are located on it. The influence of size, geometry and optical properties of the objects on the transmission spectra is numerically analyzed. We study the sensitivity of this system to changes in the refractive index of the illuminated volume induced by the presence of objects with sizes from hundreds of nanometers (submicron-sized objects) to a few microns (micron-sized objects). Parameters such as the object volume within the penetration depth of the surface plasmon in the buffer medium or the contact surface between the object and the nanostructured substrate strongly affect the sensitivity. The proposed system models the presence of objects and their detection through the spectral shifts undergone by the transmission spectra. Also, we demonstrate that these can be used for obtaining information about the refractive index of a micron-sized object immersed in a buffer and located on the nanostructured sensitive surface. We believe that results found in this study can help biomedical researchers and experimentalists in the process of detecting and monitoring biological organisms of large sizes (notably, cells).

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“…In the demolding step, the mold is detached from the molded resist and reused for the next molding cycle. Both thermal- (Im et al 2011;Kumar et al 2014;Lee et al 2015;Martinez-Perdiguero et al 2012;Martinez-Perdiguero et al 2013;Qi et al 2018), and UV-NIL (Chen et al 2009;Nakamoto et al 2012;Verschuuren et al 2015;Wong et al 2013) have been used to produce large area metallic structures for SP biosensing applications. For most cases, nanostructures were formed in a thin polymeric resist layer by NIL.…”

Section: Nanoimprint Lithography (Nil) Is a Replication Technique Utimentioning

confidence: 99%

Nanohole array plasmonic biosensors: Emerging point-of-care applications

Prasad

Choi

Jia

et al. 2019

Biosensors and Bioelectronics

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Point-of-care (POC) applications have expanded hugely in recent years and is likely to continue, with an aim to deliver cheap, portable, and reliable devices to meet the demands of healthcare industry. POC devices are designed, prototyped, and assembled using numerous strategies but the key essential features that biosensing devices require are: (1) sensitivity, (2) selectivity, (3) specificity, (4) repeatability, and (5) good limit of detection. Overall the fabrication and commercialization of the nanohole array (NHA) setup to the outside world still remains a challenge. Here, we review the various methods of NHA fabrication, the design criteria, the geometrical features, the effects of surface plasmon resonance (SPR) on sensing as well as current state-of-the-art of existing NHA sensors. This review also provides easy-to-understand examples of NHA-based POC biosensing applications, its current status, challenges, and future prospects.

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Real-Time Label-Free Surface Plasmon Resonance Biosensing with Gold Nanohole Arrays Fabricated by Nanoimprint Lithography

Cited by 27 publications

References 16 publications

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

Electromagnetic behavior of dielectric objects on metallic periodically nanostructured substrates

Nanohole array plasmonic biosensors: Emerging point-of-care applications

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