Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system

Bird, Christopher; Kirstein, Shelli L.

doi:10.1038/nmeth.f.263

Cited by 74 publications

(58 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The model extends existing models of cancer cell movement (29,30) to incorporate receptor recycling and membrane regulatory effects of MYOF, based on experimental results using xCELLigence assays (27) showing that MYOF-KD MDA-MB-231 cells exhibited decreased invasivity (Figs. 3 and 4, with immunoblot validation of MYOF specific depletion shown in SI Appendix, Fig.…”

Section: Figmentioning

confidence: 91%

“…2). We used an electrode-impedance-based invasion assay [xCELLigence (27)] to probe the effect of MYOF deficiency on cell invasion. Compared to the control MBA-MB-231 cells, MYOF-knockdown (MYOF-KD) cells exhibited reduced invasive capacity (28).…”

mentioning

confidence: 99%

“…We measure the approximate number of cells which have adhered to the microelectrode array by measuring the change in impedance, the cell index [although we note that cell index is also dependent on other factors, such as cell adhesion and spreading (27)]. To simulate these conditions, we can use a similar setup as for the conventional modified Boyden chamber simulations given in refs.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Mechanistic modeling of the effects of myoferlin on tumor cell invasion

Eisenberg

Kim

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Myoferlin (MYOF) is a member of the evolutionarily conserved ferlin family of proteins, noted for their role in a variety of membrane processes, including endocytosis, repair, and vesicular transport. Notably, ferlins are implicated in Caenorhabditis elegans sperm motility (Fer-1), mammalian skeletal muscle development and repair (MYOF and dysferlin), and presynaptic transmission in the auditory system (otoferlin). In this paper, we demonstrate that MYOF plays a previously unrecognized role in cancer cell invasion, using a combination of mathematical modeling and in vitro experiments. Using a real-time impedance-based invasion assay (xCELLigence), we have shown that lentiviral-based knockdown of MYOF significantly reduced invasion of MDA-MB-231 breast cancer cells in Matrigel bioassays. Based on these experimental data, we developed a partial differential equation model of MYOF effects on cancer cell invasion, which we used to generate mechanistic hypotheses. The mathematical model predictions revealed that matrix metalloproteinases (MMPs) may play a key role in modulating this invasive property, which was supported by experimental data using quantitative RT-PCR screens. These results suggest that MYOF may be a promising target for biomarkers or drug target for metastatic cancer diagnosis and therapy, perhaps mediated through MMPs.cancer invasion | RNAi | partial differential equation models | metastasis A majority of cancer deaths are related not to the primary tumor itself, but rather the formation of disseminated metastases (1). Cancer spread requires that cells achieve atypical motility, which enables them to invade surrounding tissues and vessels of the blood and lymphatic systems (2-4). Thus, understanding the mechanisms and signaling processes that lead to invasive cell behavior may lead to new therapeutic approaches for controlling and treating cancer.The fundamental mechanisms of invasive cancer cell movement are largely conserved across a wide range of cell types, with some of the protease dependent and protease independent movement types demonstrated by cancer cells also seen in organisms as diverse as unicellular organisms, slime molds, and white blood cells. The ferlin family is an evolutionarily ancient family of proteins (5), which are known to affect processes crucial to migration and invasion, including membrane fusion and repair, vesicle transport, endocytosis, protein recycling and stability, and cell motility (6-13). Thus, one might expect the ferlin family to be good candidates for cancer proteins, although they have not previously been investigated in this capacity. In Caenorhabditis elegans, spermatozoa exhibit amoeboid movement, and mutations in the fer-1 gene [an orthologue of myoferlin (MYOF)] result in immobility and infertility (13). In humans, MYOF has been implicated in a variety of cellular processes, including myoblast fusion, growth factor receptor stability, endocytosis, and endothelial cell membrane repair (6,8,(10)(11)(12); however until now its role in cancer cell movement ha...

show abstract

Section: Figmentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Mechanistic modeling of the effects of myoferlin on tumor cell invasion

Eisenberg

Kim

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Electrical impedance indicated as cell index (CI) value and provides quantitative information about the condition of the cells, involving cell number, viability, and morphology [9] .…”

Section: Cell Proliferation Assay With Real-time Cell Analysis Systemmentioning

confidence: 99%

“…After the incubation, HUVEC cells were seeded at a 2×104 density per well onto the top chambers of CIM-16 plates in 80 μl of serum-free media, then 100 µl of HP-1 extract concentrations (25, 50, 200 µg/ml) were added. After stabilizing CIM-plates for 30 min at room temperature, the chamber was loaded in the RTCA DP machine and software was set to collect impendence data, reported as CI values, once every 10 min for 24 h [9,11] .…”

Section: Cell Migration and Invasion Assays With Real-time Cell Analymentioning

confidence: 99%

Evaluation of Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenum Secondary Metabolites

Dikmen¹

2017

ijps

View full text Add to dashboard Cite

Dikmen, et al.: Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenumDevelopment of resistance to standard therapy and toxic side effects are the major challenges encountered in chemotherapy of colorectal cancer. Increasing evidence has shown the ability of natural products to overcome various challenging disorders. Since, the development of antibiotics, fungi have been shown to be a wide useful source of lead compounds for the development of new pharmaceuticals. Fungal secondary metabolites from extreme environments are remarkable due to their diverse components, which makes them interesting candidates for drug discovery. In order to discover novel active antimetastatic and antiangiogenic compounds, secondary metabolites of halotolerant Penicillium chrysogenum-1 isolated from Tuz Lake in Turkey and ethyl acetate extract of halotolerant P. chrysogenum-1 was prepared from the isolate of halotolerant P. chrysogenum-1 culture medium. Firstly, antioxidant activity of the extract was determined. Then cytotoxicity and cell proliferation were investigated by WST-1 assay and real-time cell analysis system-DP on human umbilical vein endothelial cells and colorectal carcinoma cells, respectively. Antiangiogenic effects on human umbilical vein endothelial cells were evaluated with cell migration and invasion assays by using real-time cell analysis system. Also, mRNA expression levels of metastasis and angiogenesis-related genes, VEGFA, VEGFB, EGFR, COX-10, ANGPT-1 and IL-8 were determined on both cell lines. Results indicated that halotolerant P. chrysogenum-1 extract exhibited significant antimetastatic and antiangiogenic effects. According to the real-time cell analysis system results, IC 50 concentrations of halotolerant P. chrysogenum-1 extract on Caco-2 and human umbilical vein endothelial cells were calculated as 55.2 and 37.89 µg/ml, respectively for 72 h. These results indicated that the secondary metabolites of halotolerant P. chrysogenum-1 extract could have the potential as a preventive and therapeutic agent in metastatic cancer processes.

show abstract

Variations in the Bioactive Compounds Composition and Biological Activities of Loofah (Luffa cylindrica) Fruits in Relation to Maturation Stages

Hlel

Belhadj

Gül

et al. 2017

Chemistry & Biodiversity

View full text Add to dashboard Cite

The present work aimed to determine the antioxidant and antiproliferative potential of Luffa cylindrica fruits collected at two different maturation stages and to identify and compare their functional components composition. The MeOH extracts of L. cylindrica fruits harvested at 60 - 65 days after seeding (S1) and 85 - 90 days after seeding (S2) were investigated for their antioxidant activity using various assays. Furthermore, the antiproliferative activity of the extracts against HeLa human cervical cancer cells was explored with xCELLigence real time cell analyzer, while the effect of the samples on the membrane integrity of the same cell line was assessed using LDH cytotoxicity leakage assay. Ultimately, the phytochemicals were analyzed using GC/MS and HPLC/TOF-MS. The S1 sample had higher contents and more diversity in the phenolic compounds composition than S2. Furthermore, the S1 extract showed the highest antioxidant and antiproliferative activity, while the S2 extract had higher cytotoxicity towards HeLa cells. The findings revealed that the time of harvest has a big impact on the phytochemicals content and activity and that harvesting L. cylindrica at an early stage before the beginning of the development of the cellulose fibrous system is recommended for a rich phytochemical composition and efficient antioxidant and antiproliferative activities.

show abstract

Real-time, label-free monitoring of cellular invasion and migration with the xCELLigence system

Cited by 74 publications

References 1 publication

Mechanistic modeling of the effects of myoferlin on tumor cell invasion

Mechanistic modeling of the effects of myoferlin on tumor cell invasion

Evaluation of Antiangiogenic and Antimetastatic Effects of Penicillium chrysogenum Secondary Metabolites

Variations in the Bioactive Compounds Composition and Biological Activities of Loofah (Luffa cylindrica) Fruits in Relation to Maturation Stages

Contact Info

Product

Resources

About