Real-time imaging of DNA–streptavidin complex formation in solution using a high-speed atomic force microscope

High-speed atomic force microscopy coming of age

“…Imaging rate, 1.03 fps (×20 playback); scan size, 800 × 800 nm 2 [98]. ・Crystallization of a CaCO 3 thin film from supersaturated solution [72] ・Ultrafast imaging of collagen [58] ・Brownian motion & photo-degradation of π-conjugated polyrotaxane [73] ・DNA translocation and looping by type III restriction enzyme [74] ・Biotinylated DNA-streptavidin interaction [75] Miles Miles Shinohara Takeyasu Trimitsu 2008 5 ・Anisotropic diffusion of point defects in streptavidin 2D crystals [76] ・Identification of intrinsically disordered regions of proteins [77] ・Human chromosomes in liquid [78] ・DNA-nuclease interaction [ ・Dynamic equilibrium at the edge of bR 2D crystals [81] ・Structural change of CaM and actin polymerization on streptavidin 2D crystals [82] ・Unidirectional translocation of cellulase along cellulose fibers [83] ・Translocation of EcoRII restriction enzyme along DNA [84] ・Fabrication and imaging of hard material surface [85] ・Purple membrane in contact-mode HS-AFM [86] ・Thermal motion of π-conjugated polymer chain [87] ・Opening of 3D hollow structure of DNA Origami [88] ・Opening of 3D hollow structure of DNA Origami [89] ・ATP-induced conformational change in P2X 4 ・Walking myosin V along an actin filament [91] ・Photo-induced structural change in bR [92] ・2D crystal formation of annexin A-V and height change of p97 [93] ・Analysis of components covering magnesotome surface [94] ・Time course of cell death by antimicrobial peptide [95] ・Dissolution of extreme UV exposed resist films under developing [96] ・Dissolution of extreme UV exposed resist films under developing [97] ・Process of forming supported planar lipid bilayer [98] ・Self assembly of amyloid-like fibrils [99] ・Effect of ClpX on FtsZ polymerization ...…”

“…Keiichi Torimitsu' group imaged the complex of biotinylated DNA and streptavidine but the complex was disrupted during imaging [75]. Using a feedback-control-free high-speed AFM apparatus developed by Mervyn Miles' group [57], Jamie Hobbs' group imaged growing polyethylene oxide films at 14 fps but the resolution was low [68].…”

Scanning probe microscopic and optical detection of DNA integration within multicomponent structures on Si surfaces

“…Thread like structures in the SPM images in Fig. 1(a) are typical of the samples based on mica substrates with DNA on it (see, e. g., [19,27,28]). Considering the influence of tip diameter on the dimensions in horizontal axis, thickness of the threads approximately represents diameter of the thread like DNA structures and is equal to about 7-8 nm.…”

High-speed AFM and nano-visualization of biomolecular processes

“…Users of the latter product recently filmed dynamic processes of GroEL-GroES interaction that were regulated by the ATPase reaction of GroEL immobilized onto mica surface in an end-up orientation [82]. Moreover, they observed formation and dissociation of a streptavidin-biotinylated DNA complex [83] and one-dimensional diffusion of a restriction enzyme along a DNA strand which was followed by the cleavage reaction [84]. A most recent study successfully captured translocation and looping of DNA by a type III restriction enzyme, EcoP15I [85].…”