Real time histochemistry of oxygen free radical production in human leukocytes.

Hirai, Kei-Ichi; Moriguchi, Keiichi; Wang, Guoying

doi:10.1267/ahc.25.319

Cited by 4 publications

(2 citation statements)

References 22 publications

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“…(a) The individual PMNL contains a great number of potential luminous areas, but after stimulation only a part of them are registered to produce light (13). So, as a luminous centre we define every space in and/or around the cell, in which a sequence of biochemical processes lead to light emission.…”

Section: Description Of the Approach Proposedmentioning

confidence: 99%

“…The composition of KRP was 119mmol/L NaCl, 4.75 mmol/L KCl, 0.420 mmol/L CaC12, 1.19 mmol/L MgS04 -7H20, 16.6 mmol/L sodium phosphate buffer, pH 7.4, and 5.56mmol/L glucose (13). All reagents used were of analytical grade and water was glass-distilled Zymosan was opsonized for 30min at 37°C in normal pooled human serum (20 mg/mL), washed twice in 0.9% NaCl and lyophilized.…”

Section: Experimental Materials a N D M E T H O D Smentioning

confidence: 99%

See 1 more Smart Citation

A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

Magrisso

Bechev

Bochev

et al. 1995

J. Biolumin. Chemilumin.

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Luminol chemiluminescence (LCL) is a simple, sensitive and time-saving tool to elucidate the oxidative activity of polymorphonuclear leukocytes (PMNL). In this study, a new approach for analysing the LCL kinetics, recorded from stimulated PMNL, and for a more accurate elucidation of their functional state, is proposed. This approach is based on the proposal that the LCL kinetics of stimulated PMNL is a result of time-probabilistic nature of the processes, leading to light emission. On this basis the LCL response was described by the parameters of a Poisson-type distribution. Relationships between these parameters and some factors influencing LCL kinetics (number of PMNL and erythrocytes, temperature, stirring) were investigated. The model descriptions of the LCL responses obtained had two components (modes). Their development in time was different. It was suggested, that the two phases of the model LCL response were connected with extracellular and intracellular LCL. The terms and the advantages of this approach for analysis of phagocyte oxidative capacity are discussed.

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Section: Description Of the Approach Proposedmentioning

confidence: 99%

Section: Experimental Materials a N D M E T H O D Smentioning

confidence: 99%

A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

Magrisso

Bechev

Bochev

et al. 1995

J. Biolumin. Chemilumin.

View full text Add to dashboard Cite

show abstract

Model components of luminol chemiluminescence generated by PMNL

Magrisso¹,

Alexandrova²,

Bochev³

et al. 1995

Journal of Biochemical and Biophysical Methods

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Histochemical Visualization of Oxidant Stress

FRANK¹,

POMPELLA²,

BIESALSKI³

2001

Bio-Assays for Oxidative Stress Status

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Free radicals induce oxidative modification in distinct components of the living matter (lipid, proteins, and DNA). For qualitative and quantitative determination of free radical-induced modifications, different, more or less sensitive biochemical methods are available. Because of the high reactivity and short life of free radicals, ongoing oxidative damage is generally analyzed by measurement of secondary products-such as H 2 0 2 , oxidized proteins, peroxidized lipids, and their breakdown products, oxidized DNA-or by fluorographic analysis in combination with fluorescent dyes such as dichlorofluorescin (DCFH). In addition, the determination of free radical-related oxidation products is usually carried out in plasma, urine, or, less frequently, in bioptic material. Consequently, biochemical data seldom reflect the effects of free radical insults in situ. The histochemical visualization of selected molecular markers of oxidative damage can often provide more valuable information concerning the in vivo distribution of oxidative processes. This review summarizes the methods currently available for histochemical detection and indirect visualization of free radical-induced alterations in tissues and isolated cells.

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Real time histochemistry of oxygen free radical production in human leukocytes.

Cited by 4 publications

References 22 publications

A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

A new approach for analysis of chemiluminescent kinetics of activated phagocytes in blood

Model components of luminol chemiluminescence generated by PMNL

Histochemical Visualization of Oxidant Stress

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