Model components of luminol chemiluminescence generated by PMNL

Magrisso, Moni J.; Alexandrova, Margarita L.; Bochev, Petyo G.; Bechev, Blagovest; Markova, Vania I.; Benchev, Ilia C.

doi:10.1016/0165-022x(95)00014-5

Cited by 14 publications

(14 citation statements)

References 8 publications

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“…The diarylpentanoid analogues of curcumin were synthesized by direct coupling of the appropriate aromatic aldehyde with the three ketones, acetone (1-17), cyclohexanone (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33) or cyclopentanone (34-43) at a molar ratio of 1 : 2, using the base-catalysed Claisen-Schmidt condensation reaction. Figure 1 illustrates the general synthesis of diarylpentanoids.…”

Section: Synthesis Of Diarylpentanoid Analoguesmentioning

confidence: 99%

“…The molecular weight of luminol is relatively small and it can enter the cells and then react with intracellular ROS. [26] For the assay, 25 ml diluted whole blood (1 : 50 dilution in sterile PBS, pH 7.4) or 25 ml PMN (1 ¥ 10 6 /ml) suspended in HBSS ++ were incubated with 25-ml serial dilutions of synthetic compounds in DMSO and H2O (5 : 95 ratio) (6.25-100.00 mg/ml). The cells were stimulated with 25 ml of opsonized zymosan followed by 25 ml of luminol (7 ¥ 10 5 m) and then HBSS ++ was added to adjust the final volume to 200 ml.…”

Section: Cell Viabilitymentioning

confidence: 99%

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Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes

Jantan

Bukhari

Lajis

et al. 2011

Journal of Pharmacy and Pharmacology

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Section: Synthesis Of Diarylpentanoid Analoguesmentioning

confidence: 99%

Section: Cell Viabilitymentioning

confidence: 99%

Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes

Jantan

Bukhari

Lajis

et al. 2011

Journal of Pharmacy and Pharmacology

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“…6). Such an effect is analogous to that observed in the total LCL at a lower stimulant concentration, which is considered predominantly intracellular (32). It is known that the inhibition of cyclooxygenase-1 by piroxicam leads to the accumulation of free arachidonic acid, which is able to directly activate the NADPH-oxidase through a protein kinaseindependent pathway, which may explain the reversal of the effect.…”

Section: Piroxicam Enhances Zymosan-stimulated Intracellular Lcl (Intmentioning

confidence: 78%

Inhibitory and enhancing effects of piroxicam on whole blood chemiluminescence

2006

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The effects of piroxicam on the production of reactive oxygen species by stimulated phagocytes was studied in whole blood by a chemiluminescence (CL) technique in relation to maximum activity, localization and kinetics of radical generation. We found that piroxicam dose-dependently inhibited total (intra- and extracellular) zymosan-stimulated luminol CL (LCL) at a high stimulant concentration (p = 0.0001). Piroxicam additionally decreased cytochalasin B-reduced LCL, which shows that the effect of the drug should be sought in the extracellular component of the response. Piroxicam inhibited the first phase of extracellular LCL in a dose-dependent manner (p = 0.0001) and revealed itself as an enhancing agent of CL in later time intervals after the start of respiratory burst, in a model system containing horseradish peroxidase (HRP) and sodium azide. It enhanced LCL of a cell-free system, i.e. influenced the CL due to HRP-catalysed decomposition of hydrogen peroxide. It also dose-dependently inhibited the early extracellular superoxide production, evaluated by lucigenin CL (p = 0.022). Piroxicam inhibited the total fMLP-stimulated LCL by 70% approximately and, only by about 30%, the first phase of fMLP-stimulated extracellular LCL, which presupposes an effect on myeloperoxidase-catalysed formation of hypochloric acid. Piroxicam slightly increased the intracellular LCL by phagocytes (p = 0.02), an effect that is probably connected with its ability to induce the release of secondary messengers in signal transduction. In conclusion, the anti-inflammatory effect of piroxicam is probably related to the inhibition of the extracellular generation of superoxide and hypochloric acid in the early stages of phagocyte activation.

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“…The model components of the total CL kinetics are plotted in the same figure. The cellular-biochemical characteristics of the three components were summarized in our previous studies (13) as follows:…”

Section: Assaysmentioning

confidence: 99%

“…The registered CL kinetics result from the contributions of the different processes (10,11) and can be considered as the sum of kinetics (components), which differ in their time courses and reflect the contribution of various sequences of processes leading to CL (12). As has been reported (13), a component approach for analysis of CL kinetics is appropriate for an estimation of the respiratory burst.…”

Section: Introductionmentioning

confidence: 99%

Functional states of polymorphonuclear leukocytes determined by chemiluminescent kinetic analysis

Magrisso¹,

Alexandrova²,

Markova³

et al. 2000

Luminescence

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During the respiratory burst, upon stimulation with both soluble and particulate matter, polymorphonuclear leukocytes (PMN) generate reactive oxygen species (ROS) and emit chemiluminescence (CL) as a result of metabolic activation. The measurement of CL has been demonstrated to be a useful tool for in vitro assessment of the opsonophagocytic function of PMN. Using component analysis of CL kinetics, we characterized the functional state of PMN by three parameters of the respiratory burst: capacity, effectiveness and velocity (CEV space). The possibility of delimiting eight different functional states of PMN is discussed. The CL kinetics shown by blood PMN in different functional states was analysed, and revealed six out of eight functional states. We conclude that CEV-estimated functional states of PMN are relative, depending on both PMN readiness to generate ROS and conditions of the CL test.

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Model components of luminol chemiluminescence generated by PMNL

Cited by 14 publications

References 8 publications

Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes

Effects of diarylpentanoid analogues of curcumin on chemiluminescence and chemotactic activities of phagocytes

Inhibitory and enhancing effects of piroxicam on whole blood chemiluminescence

Functional states of polymorphonuclear leukocytes determined by chemiluminescent kinetic analysis

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