Functional states of polymorphonuclear leukocytes determined by chemiluminescent kinetic analysis

Abstract

During the respiratory burst, upon stimulation with both soluble and particulate matter, polymorphonuclear leukocytes (PMN) generate reactive oxygen species (ROS) and emit chemiluminescence (CL) as a result of metabolic activation. The measurement of CL has been demonstrated to be a useful tool for in vitro assessment of the opsonophagocytic function of PMN. Using component analysis of CL kinetics, we characterized the functional state of PMN by three parameters of the respiratory burst: capacity, effectiveness and velocity (CEV space). The possibility of delimiting eight different functional states of PMN is discussed. The CL kinetics shown by blood PMN in different functional states was analysed, and revealed six out of eight functional states. We conclude that CEV-estimated functional states of PMN are relative, depending on both PMN readiness to generate ROS and conditions of the CL test.

Luminol‐dependent chemiluminescence of human phagocyte cell lines: comparison between DMSO differentiated PLB 985 and HL 60 cells

“…The peak value in the LCL response curve was expressed as peak height (PH), which is the same as maximal LCL intensity. The area under the curve (AUC) resembles the capacity of the cells to generate ROS (24,31). Addition of catalase and SOD to the reaction mixture almost abolished the LCL response in both cell lines (Fig.…”