Real-time fluorescence loop-mediated isothermal amplification assay for rapid and sensitive detection of Streptococcus gallolyticus subsp. gallolyticus associated with colorectal cancer

Lin, Qiuyuan; Ye, Xin; Yang, Bin; Fang, Xueen; Chen, Hui; Weng, Wenhao; Kong, Jilie

doi:10.1007/s00216-019-02059-8

Cited by 11 publications

(7 citation statements)

References 39 publications

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“…In terms of other nucleic acid tests, loop-mediated isothermal amplification (LAMP) has been shown as a promising alternative to PCR [8,9] and has been reported to be an effective diagnostic assay for a variety of infectious diseases [10,11]. LAMP-based DNA amplification has been reported to be one of the most efficient isothermal amplification techniques, capable of competing with PCR and not requiring such expensive thermocycle control [12,13].…”

Section: Introductionmentioning

confidence: 99%

Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

et al. 2022

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Bacillus stearothermophilus large fragment (BSTLF) DNA polymerase is reported, isolated on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry (mCh), included in the fusion construct, was shown as an efficient fluorescent label to follow the workflow from gene to diagnostic. The R5 immobilisation on silica from cell lysate was consistent with cooperative R5-specific binding of R52-mCh-FL-BSTLF or R52-mCh-H10-BSTLF fusion proteins followed by non-specific protein binding (including E. coli native proteins). Higher R5-binding could be achieved in the presence of phosphate, but phosphate residue reduced loop-mediated isothermal amplification (LAMP) performance, possibly blocking sites on the BSTLF for binding of β- and γ-phosphates of the dNTPs. Quantitative assessment showed that cations (Mg2+ and Mn2+) that complex the PPi product optimised enzyme activity. In malaria testing, the limit of detection depended on Plasmodium species and primer set. For example, 1000 copies of P. knowlesi 18S rRNA could be detected with the P.KNO-LAU primer set with Si-R52-mCh-FL-BSTLF , but 10 copies of P. ovale 18S rRNA could be detected with the P.OVA-HAN primer set using the same enzyme. The Si-immobilised BSTLF outperformed the commercial enzyme for four of the nine Plasmodium LAMP primer sets tested. Si-R52-mCh-FL-BSTLF production was transferred from Cambridge to Accra and set up de novo for a trial with clinical samples. Different detection limits were found, targeting the mitochondrial DNA or the 18S rRNA gene for P. falciparum. The results are discussed in comparison with qPCR and sampling protocol and show that the Si-BSTLF polymerase can be optimised to meet the WHO recommended guidelines. Graphical abstract

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Section: Introductionmentioning

confidence: 99%

Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

et al. 2022

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“…An SGG infection has been associated with the development of colorectal cancer [11,32,34] but is more common in early adenomas than in developed cancers. Lin et al [11] indicated that 65 % of patients with an invasive SGG infection were additionally burdened with colorectal cancer. The presence of SGG in people with colon cancer was ten times higher than in the healthy population [11].…”

Section: Streptococcus Gallolyticus Subsp Gallolyticus (Sgg)mentioning

confidence: 99%

“…Lin et al [11] indicated that 65 % of patients with an invasive SGG infection were additionally burdened with colorectal cancer. The presence of SGG in people with colon cancer was ten times higher than in the healthy population [11]. Butt et al [12] suggest that SGG influences the development of neoplasms that have already arisen in the human body.…”

Section: Streptococcus Gallolyticus Subsp Gallolyticus (Sgg)mentioning

confidence: 99%

“…An SGG infection has been associated with the development of colorectal cancer [11, 32, 34] but is more common in early adenomas than in developed cancers. Lin et al .…”

Section: Introductionmentioning

confidence: 99%

“…Due to the high genetic variability and the open pangenome of streptococci, there is a likelihood of rapid adaptation of microorganisms to the human host [8,9]. The inclusion of new species of streptococci into the human microbiome may also lead to changes in its metabolome that may favour non-infectious diseases, including cancer [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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Pathogenicity and drug resistance of animal streptococci responsible for human infections

Glajzner

Szewczyk

Szemraj

2021

Journal of Medical Microbiology

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Bacteria of the genus Streptococcus , earlier considered typically animal, currently have also been causing infections in humans. It is necessary to make clinicians aware of the emergence of new species that may cause the development of human diseases. There is an increasing frequency of isolation of streptococci such as S. suis , S. dysgalactiae , S. iniae and S. equi from people. Isolation of Streptococcus bovis/Streptococcus equinus complex bacteria has also been reported. The streptococcal species described in this review are gaining new properties and virulence factors by which they can thrive in new environments. It shows the potential of these bacteria to changes in the genome and the settlement of new hosts. Information is presented on clinical cases that concern streptococcus species belonging to the groups Bovis, Pyogenic and Suis. We also present the antibiotic resistance profiles of these bacteria. The emerging resistance to β-lactams has been reported. In this review, the classification, clinical characteristics and antibiotic resistance of groups and species of streptococci considered as animal pathogens are summarized.

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Development of multiplex HRM-based loop-mediated isothermal amplification method for specific and sensitive detection of Treponema pallidum

et al. 2022

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Real-time fluorescence loop-mediated isothermal amplification assay for rapid and sensitive detection of Streptococcus gallolyticus subsp. gallolyticus associated with colorectal cancer

Cited by 11 publications

References 39 publications

Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis

Pathogenicity and drug resistance of animal streptococci responsible for human infections

Development of multiplex HRM-based loop-mediated isothermal amplification method for specific and sensitive detection of Treponema pallidum

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