Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells

Lee, Rimi; Kim, Il Sun; Han, Nalae; Yun, Seokhwan; Park, Kook In; Yoo, Kyung Hwa

doi:10.1038/srep06319

Cited by 15 publications

(8 citation statements)

References 30 publications

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“…EIS has been utilized already to distinguish initially differentiated cells from their progenitors, but these studies concentrate only on one single impedance parameter (resistance or capacitance) and do not consider neuronal networks structures as well as a sufficient investigation period to qualitatively and quantitatively track the complete maturation process (Lee et al 2014;Park et al 2011). Another approach proved that neurons, astrocytes and their progenitors in suspension have different dielectric properties suitable for cell sorting (Flanagan et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Seidel

Obendorf

Englich

et al. 2016

Biosensors and Bioelectronics

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In today's neurodevelopment and -disease research, human neural stem/progenitor cell-derived networks represent the sole accessible in vitro model possessing a primary phenotype. However, cultivation and moreover, differentiation as well as maturation of human neural stem/progenitor cells are very complex and time-consuming processes. Therefore, techniques for the sensitive non-invasive, real-time monitoring of neuronal differentiation and maturation are highly demanded. Using impedance spectroscopy, the differentiation of several human neural stem/progenitor cell lines was analyzed in detail. After development of an optimum microelectrode array for reliable and sensitive long-term monitoring, distinct cell-dependent impedimetric parameters that could specifically be associated with the progress and quality of neuronal differentiation were identified. Cellular impedance changes correlated well with the temporal regulation of biomolecular progenitor versus mature neural marker expression as well as cellular structure changes accompanying neuronal differentiation. More strikingly, the capability of the impedimetric differentiation monitoring system for the use as a screening tool was demonstrated by applying compounds that are known to promote neuronal differentiation such as the γ-secretase inhibitor DAPT. The non-invasive impedance spectroscopy-based measurement system can be used for sensitive and quantitative monitoring of neuronal differentiation processes. Therefore, this technique could be a very useful tool for quality control of neuronal differentiation and moreover, for neurogenic compound identification and industrial high-content screening demands in the field of safety assessment as well as drug development.

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Section: Discussionmentioning

confidence: 99%

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Seidel

Obendorf

Englich

et al. 2016

Biosensors and Bioelectronics

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“…Several studies have shown that EFs induce neurite elongation of neural cells [ 2 , 38 – 40 ]. In the work by Hinkle et al ., Xenopus spinal neurons were cultured in the presence of EFs.…”

Section: Discussionmentioning

confidence: 99%

“…In the work by Li et al ., steady and pulsed DC EF stimulation directed the neurite extension of spiral ganglion neurons [ 2 ]. In the work by Lee et al ., some neurites extended after AC/DC stimulation on human NSCs cultured in differentiation medium [ 40 ]. In our study, the primary processes and the branches of mNPCs were clearly evident after stimulation by DC pulses for 48 h. Fig 3 demonstrates our finding that DC pulses promoted the elongated processes of mNPCs.…”

Section: Discussionmentioning

confidence: 99%

Pulsed DC Electric Field–Induced Differentiation of Cortical Neural Precursor Cells

et al. 2016

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We report the differentiation of neural stem and progenitor cells solely induced by direct current (DC) pulses stimulation. Neural stem and progenitor cells in the adult mammalian brain are promising candidates for the development of therapeutic neuroregeneration strategies. The differentiation of neural stem and progenitor cells depends on various in vivo environmental factors, such as nerve growth factor and endogenous EF. In this study, we demonstrated that the morphologic and phenotypic changes of mouse neural stem and progenitor cells (mNPCs) could be induced solely by exposure to square-wave DC pulses (magnitude 300 mV/mm at frequency of 100-Hz). The DC pulse stimulation was conducted for 48 h, and the morphologic changes of mNPCs were monitored continuously. The length of primary processes and the amount of branching significantly increased after stimulation by DC pulses for 48 h. After DC pulse treatment, the mNPCs differentiated into neurons, astrocytes, and oligodendrocytes simultaneously in stem cell maintenance medium. Our results suggest that simple DC pulse treatment could control the fate of NPCs. With further studies, DC pulses may be applied to manipulate NPC differentiation and may be used for the development of therapeutic strategies that employ NPCs to treat nervous system disorders.

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“…Neural stem cells were shown to have differences in time-course capacitance when cultured on interdigitated capacitance sensors and cultured under different conditions [70]. Proliferation and differentiation into either neuronal or astroglial cells could be differentiated in real time.…”

Section: (Ii) Pluripotent Stem Cellsmentioning

confidence: 99%

Impedance-based cellular assays for regenerative medicine

Gamal

Underwood

et al. 2018

Phil. Trans. R. Soc. B

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Therapies based on regenerative techniques have the potential to radically improve healthcare in the coming years. As a result, there is an emerging need for non-destructive and label-free technologies to assess the quality of engineered tissues and cell-based products prior to their use in the clinic. In parallel, the emerging regenerative medicine industry that aims to produce stem cells and their progeny on a large scale will benefit from moving away from existing destructive biochemical assays towards data-driven automation and control at the industrial scale. Impedance-based cellular assays (IBCA) have emerged as an alternative approach to study stem-cell properties and cumulative studies, reviewed here, have shown their potential to monitor stem-cell renewal, differentiation and maturation. They offer a novel method to non-destructively assess and quality-control stem-cell cultures. In addition, when combined with disease models they provide complementary insights as label-free phenotypic assays. IBCA provide quantitative and very sensitive results that can easily be automated and up-scaled in multi-well format. When facing the emerging challenge of real-time monitoring of three-dimensional cell culture dielectric spectroscopy and electrical impedance tomography represent viable alternatives to two-dimensional impedance sensing.This article is part of the theme issue 'Designer human tissue: coming to a lab near you'.

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Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells

Cited by 15 publications

References 30 publications

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Pulsed DC Electric Field–Induced Differentiation of Cortical Neural Precursor Cells

Impedance-based cellular assays for regenerative medicine

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