2018
DOI: 10.1098/rstb.2017.0226
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Impedance-based cellular assays for regenerative medicine

Abstract: Therapies based on regenerative techniques have the potential to radically improve healthcare in the coming years. As a result, there is an emerging need for non-destructive and label-free technologies to assess the quality of engineered tissues and cell-based products prior to their use in the clinic. In parallel, the emerging regenerative medicine industry that aims to produce stem cells and their progeny on a large scale will benefit from moving away from existing destructive biochemical assays towards data… Show more

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Cited by 50 publications
(44 citation statements)
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“…Metals 2020, 10, x FOR PEER REVIEW 3 of 14 approach, impedance-based cellular assays in the field of tissue engineering and regenerative medicine offer a range of methods that use microelectrodes to measure the impedance of biological systems, and thus obtain information of cellular behavior growing on any surface or biomaterials [49]. In this work, it is studied and discussed the use of electrical impedance spectroscopy to characterize both the porosity size of porous titanium discs produced by space holder technique and the growth of osteoblast cell cultures on the flat surface and inside of pores.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Metals 2020, 10, x FOR PEER REVIEW 3 of 14 approach, impedance-based cellular assays in the field of tissue engineering and regenerative medicine offer a range of methods that use microelectrodes to measure the impedance of biological systems, and thus obtain information of cellular behavior growing on any surface or biomaterials [49]. In this work, it is studied and discussed the use of electrical impedance spectroscopy to characterize both the porosity size of porous titanium discs produced by space holder technique and the growth of osteoblast cell cultures on the flat surface and inside of pores.…”
Section: Methodsmentioning
confidence: 99%
“…Traditionally, the techniques that are required to evaluate and monitor the renewal, differentiation and maturation of osteoblast cells in culture after different experimental conditions are generally destructive techniques, such as histology, scanning electron microscopy, fluorescence microscopy, immunohistochemistry and other biochemical assays. Recently, as an alternative approach, impedance-based cellular assays in the field of tissue engineering and regenerative medicine offer a range of methods that use microelectrodes to measure the impedance of biological systems, and thus obtain information of cellular behavior growing on any surface or biomaterials [49]. In this work, it is studied and discussed the use of electrical impedance spectroscopy to characterize both the porosity size of porous titanium discs produced by space holder technique and the growth of osteoblast cell cultures on the flat surface and inside of pores.…”
Section: Introductionmentioning
confidence: 99%
“…Thus, by measuring the impedance changes between two electrodes, information related to cell number, shape and their motility can be obtained [16]. Starting from the reliability already demonstrated by this technique in 2D cell culture-which led also to commercially available impedance-based systems [17]-in the last decades a huge interest has been addressed to the possibility of translating this approach towards 3D settings [18].…”
Section: Introductionmentioning
confidence: 99%
“…The electrical cell-substrate impedance sensing technique (ECIS) is a well-established method that allows the real time acquisition of biological parameters (cell growth, motility, activity, or size) of any cell culture and its relationship with the environment through bioimpedance measurements [4]. It can be used in many different studies, such as in toxicity studies, cell growth, cell motility [4,5], cancer progression [6], or stem cell differentiation for regenerative medicine [7].…”
Section: Introductionmentioning
confidence: 99%