Real-time Detection of Single Immobilized Nanoparticles by Surface Plasmon Resonance Imaging

Abstract

In this manuscript, a new approach in surface plasmon resonance microscopy is presented. The method provides optical real-time detection of single nanoparticles on surfaces. The potential of the method is demonstrated recording spherical dielectric particles as small as 40 nm in diameter and single HIV virus-like particles having diameters of ∼100 nm both immobilized on functionalized surfaces. The surface plasmon resonance signal in the binding spots was found to be almost linearly proportional to the size of the particles and, therefore, surpasses the intensity of Mie scattering on spherical particle (dependence ∼ r −6 ) by orders of magnitude for small objects. The physical reason leading to this strong effect is discussed.

Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles

“…Only recent groundbreaking studies performed in independent working groups helped in realizing the potential of SPR-based sensors in studies of biological submicrometer and nanoparticles [4][5][6][7]. Zybin and colleagues used SPR-based ''microscopy'' (also called ''SPR nanoscopy'') to detect the binding of individual polystyrene particles of different sizes (minimal used size was 40 nm diameter) to the functionalized sensor surface [6].…”

Control charts for the mean based on robust two-sample tests

“…Generally, the deviation to the N (0, 1)-curve gets smaller with increasing α for all considered procedures. The scale estimatorŜ (4) used for the Hodges-Lehmann based procedures, seems to result in a somewhat smaller difference to the N (0, 1)-curve. In the investigated situations, the randomization principle can lead to approximately distribution-free procedures, but this is not guaranteed.…”

“…If the coordinate is part of a virus adhesion, it has a sudden positive or negative jump depending on whether the coordinate belongs to the bright spot or the dark circle. More details on the sensor can be found in [4] or [5]. Figure 1(b) shows a time series from a PAMONO data set.…”

Detection and Digital Resolution Counting of Nanoparticles with Optical Resonators and Applications in Biosensing

“…However, prism-based systems can be physically bulky and limited in their spatial resolution, and their long working distance can produce image distortion [126,127]. To address these issues, Zare et al first demonstrated SPR imaging using a high numerical aperture objective, as shown in Figure 5a [128].…”