Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles

Shpacovitch, Victoria; Temchura, Vladimir; Matrosovich, Mikhail; Hamacher, J.; Skolnik, Julia; Libuschewski, Pascal; Siedhoff, Dominic; Weichert, Frank; Marwedel, Peter; Müller, Heinrich; Überla, Klaus; Hergenröder, Roland; Zybin, A.

doi:10.1016/j.ab.2015.06.022

Cited by 34 publications

(32 citation statements)

References 20 publications

(41 reference statements)

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“…In previous studies [9], we used biotin-thiol, streptavidin and biotinylated anti-analyte antibody self-assembling layers for sensor surface functionalization. However, this approach has several disadvantages: necessity to use biotin-conjugated antibodies, single-use gold sensor and a lack of oriented immobilization of anti-target antibody onto sensor surface, which, in turn, can affect the efficiency of targeted vesicle capture.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, we demonstrated the linearity of calibration between concentration and counting rate for HIV-VLPs [9]. Therefore, we hypothesized here that samples containing MVs can be compared with each other even without establishing a calibration curve.…”

Section: Resultsmentioning

confidence: 99%

“…The sensor surface was functionalized with an anti-OVA antibody giving an opportunity to estimate this specificity. Evaluation of antigen-based specificity has been previously addressed in our studies [9]. However, in the earlier work, another type of sensor surface functionalization was employed (biotin-thiol-streptavidin-biotinylated antibody) and the corresponding concentrations of VLPs were determined using an indirect method—Enzyme-linked Immunosorbent Assay (ELISA).…”

Section: Resultsmentioning

confidence: 99%

“…Only recently, results from independent research teams proved the applicability of SPR-based sensors in studies of biological nano-objects [1,2,7,8]. The PAMONO-sensor was demonstrated to be an appropriate tool for detection and quantification of individual viruses, virus-like particles and inorganic nano-particles [9]. It is also not surprising that SPR-based sensors were recently [10,11] tried out in studies focused on detection and quantification of another group of biological nano-vesicles—extracellular vesicles.…”

Section: Introductionmentioning

confidence: 99%

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Application of the PAMONO-Sensor for Quantiﬁcation of Microvesicles and Determination of Nano-Particle Size Distribution

Shpacovitch

Sidorenko²,

Lenssen

et al. 2017

Sensors

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Abstract:The PAMONO-sensor (plasmon assisted microscopy of nano-objects) demonstrated an ability to detect and quantify individual viruses and virus-like particles. However, another group of biological vesicles-microvesicles (100-1000 nm)-also attracts growing interest as biomarkers of different pathologies and needs development of novel techniques for characterization. This work shows the applicability of a PAMONO-sensor for selective detection of microvesicles in aquatic samples. The sensor permits comparison of relative concentrations of microvesicles between samples. We also study a possibility of repeated use of a sensor chip after elution of the microvesicle capturing layer. Moreover, we improve the detection features of the PAMONO-sensor. The detection process utilizes novel machine learning techniques on the sensor image data to estimate particle size distributions of nano-particles in polydisperse samples. Altogether, our findings expand analytical features and the application field of the PAMONO-sensor. They can also serve for a maturation of diagnostic tools based on the PAMONO-sensor platform.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Application of the PAMONO-Sensor for Quantiﬁcation of Microvesicles and Determination of Nano-Particle Size Distribution

Shpacovitch

Sidorenko²,

Lenssen

et al. 2017

Sensors

Self Cite

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“…The imaging intensities of the viral particles were used to determine their size, which was found as 109 ± 13 nm. In another study, viral particles were counted using SPRi [35]. When the viral particles were captured on the sensor surface, they appeared as bright dots on the background and counted in a very small field of view to improve the detection limit and block the signals coming from the other regions.…”

Section: Importance Of Surface Morphology In Single Particle Optical mentioning

confidence: 99%

Surface chemistry and morphology in single particle optical imaging

et al. 2017

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Biological nanoparticles such as viruses and exosomes are important biomarkers for a range of medical conditions, from infectious diseases to cancer. Biological sensors that detect whole viruses and exosomes with high specificity, yet without additional labeling, are promising because they reduce the complexity of sample preparation and may improve measurement quality by retaining information about nanoscale physical structure of the bio-nanoparticle (BNP). Towards this end, a variety of BNP biosensor technologies have been developed, several of which are capable of enumerating the precise number of detected viruses or exosomes and analyzing physical properties of each individual particle. Optical imaging techniques are promising candidates among broad range of label-free nanoparticle detectors. These imaging BNP sensors detect the binding of single nanoparticles on a flat surface functionalized with a specific capture molecule or an array of multiplexed capture probes. The functionalization step confers all molecular specificity for the sensor's target but can introduce an unforeseen problem; a rough and inhomogeneous surface coating can be a source of noise, as these sensors detect small local changes in optical refractive index. In this paper, we review several optical technologies for label-free BNP detectors with a focus on imaging systems. We compare the surface-imaging methods including dark-field, surface plasmon resonance imaging and interference reflectance imaging. We discuss the importance of ensuring consistently uniform and smooth surface coatings of capture molecules for these types of biosensors and finally summarize several methods that have been developed towards addressing this challenge.

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Bioreceptors for Cells

Prajapati,

Roy

2023

Biosensors Nanotechnology

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Application of surface plasmon resonance imaging technique for the detection of single spherical biological submicrometer particles

Cited by 34 publications

References 20 publications

Application of the PAMONO-Sensor for Quantiﬁcation of Microvesicles and Determination of Nano-Particle Size Distribution

Application of the PAMONO-Sensor for Quantiﬁcation of Microvesicles and Determination of Nano-Particle Size Distribution

Surface chemistry and morphology in single particle optical imaging

Bioreceptors for Cells

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