Real-time detection of mRNA splicing variants with specifically designed reverse-transcription loop-mediated isothermal amplification

Abstract

A novel splicing variant assay is developed based on specifically designed reverse-transcription (RT) loop-mediated isothermal amplification.

Novel reverse transcription-multiple inner primer loop-mediated isothermal amplification (RT-MIPLAMP) for visual and sensitive detection of SARS-CoV-2

“…In addition, the four primers identify six specic regions in the RNA template, ensuring the high specicity of LAMP-based approaches. Because of these superiorities of RT-LAMP, it has been recruited to assay mRNA, 16 single nucleotide polymorphism (SNP) 17 and telomerase. 18 In the midst of the COVID-19 pandemic, RT-LAMP has also been studied to develop diagnostic tests for SARS-CoV-2 RNA, such as SARS-CoV-2 DNA Endonuclease-Targeted CRISPR Trans Reporter (DETECTR) assay, 19 colorimetric RT-LAMP assay 20,21 and portable assays, 22,23 and thus, it is hoped to establish a rapid, simple and cost-effective assay for SARS-CoV-2 based on RT-LAMP.…”