Real-time analysis of sulfur-containing volatiles in Brassica plants infested with root-feeding Delia radicum larvae using proton-transfer reaction mass spectrometry

Abstract

Proton Transfer Reaction-Mass Spectrometry (PTR-MS) analyses revealed that damaged Brassica roots emit sulfur-containing volatiles. B. nigra, B. juncea and B. napus emitted isothiocyanate markers, whereas B. rapa, B. oleracea, and B. carinata emitted methanethiol. These compounds can be used as markers for root damage by insect larvae and other below-ground herbivores.

Mechanosensitivity below Ground: Touch-Sensitive Smell-Producing Roots in the Shy Plant Mimosa pudica

“…Of particular relevance is the finding that the changing profile of organosulfur exudates that occurs in Brassica spp. roots in response to herbivore attack or a tissue breach can be monitored in real time by proton transfer reaction-mass spectrometry (Crespo et al, 2012;Danner et al, 2012;van Dam et al, 2012;Samudrala et al, 2015). If conventional metabolome analysis sample preparation methods had been used in these cases (e.g.…”

Myzus persicae Feeding on Water Stressed-Arabidopsis Affects the Emission Profile of Plant Volatile Organic Compounds

“…Published studies found that in Brassica species, the enzymatic thiol methyltransferase (TMT) have a strong influence on the hydrolysis of GS products to volatile sulfides (e.g., DMDS and dimethyl trisulfide (DMTS)) (Attieh et al, 2002;van Dam et al, 2012). It is reported that the artificial and natural damage to B. napus (L.) and B. juncea (L.) roots led to highest TMT activities in their leaves (van Dam et al, 2012).…”

“…It is reported that the artificial and natural damage to B. napus (L.) and B. juncea (L.) roots led to highest TMT activities in their leaves (van Dam et al, 2012). Existing data indicate that the physical conditions and herbivorous insects are extremely potent in the release of sulfur volatiles (Dugravot et al, 2005;Rouseff et al, 2008).…”

Optimization and sensitive detection of sulfur compounds emitted from plants using proton transfer reaction mass spectrometry

“…This verifies the need to optimize the settings of the instrument before the start of the plant experiments. Further details and explanation about the biological background of the experiments can be found in van Dam et al [8].…”

“…The latter makes possible real time analysis and simultaneous monitoring of several volatile compounds. Owing to these advantages compounds that are typical of Brassicaceae family species [8]. Glucosinolates are non-volatile compounds.…”