Real-time analysis of microbial growth by means of the Heat-Transfer Method (HTM) using Saccharomyces cerevisiae as model organism

Betlem, Kaï; Hoksbergen, S.; Mansouri, N.; Down, Michael P.; Losada-Pérez, Patricia; Eersels, Kasper; Grinsven, Bart van; Cleij, Thomas J.; Kelly, Peter; Sawtell, David; Zubko, Mikhajlo K.; Banks, Craig E.; Peeters, Marloes

doi:10.1016/j.phmed.2018.05.001

Cited by 16 publications

(23 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For digestate sample 3 (originating from a brewery), additional platings were performed on YEPD plates due to the high presence of yeast in these samples as part of the beer brewing process. The incubation period was extended to two days due to slower microbial growth of the bacteria from this sample, which is line with samples analysed previously containing yeast [22].…”

Section: Mediasupporting

confidence: 83%

“…Recently, Betlem et al, [22] demonstrated that it is possible to study growth of yeast in realtime with the use of gold electrodes. In order to facilitate longitudinal experiments, it was necessary to adapt the flow cell design.…”

Section: Methodsmentioning

confidence: 99%

“…The effect of a continuous flow of nutrients (flow rate = 1 mL/h) on the thermal response of the gold-coated electrodes over a prolonged time was evaluated. This flow rate based on results from previous work [22] ensured minimal disturbance in the thermal resistance signal while still providing sufficient nutrients for the microorganisms to grow. There was no significant effect on the thermal resistance and a clear baseline was established ( Figure S2).…”

Section: Evaluation Of S Aureus Growth In the Nutrient Brothmentioning

confidence: 99%

See 2 more Smart Citations

Heat-Transfer Method: A Thermal Analysis Technique for the Real-Time Monitoring of Staphylococcus aureus Growth in Buffered Solutions and Digestate Samples

Betlem

Kaur

Hudson

et al. 2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

The identification and quantification of microorganisms in water samples is crucial to improve processes in organic waste treatment facilities. Most of the currently available tests are either labour-intense or costly, and they do not allow determination of the dynamics within microbial communities in digestate samples. This study is the first report on the use of thermal analysis, specifically the Heat Transfer Method (HTM), to monitor microbial load in aqueous systems and digestate samples. Staphylococcus aureus was used as a model organism and different concentrations in water were measured by HTM. It was demonstrated that there was a positive correlation between the thermal resistance and concentration of the bacterial cells. Subsequently, the influence of temperature on growth rates was studied and confirmed by plating experiments and Scanning Electron Microscopy (SEM). These results showed the possibility to monitor the temperature dependent growth of S. aureus using HTM. To determine if this technique can be applied for studying complex matrices, digestate samples were collected from a number of sources and plated on nutrient agar plates. The bacterial cultures derived from 2 single colonies were characterised and identified by sequencing of DNA regions for 16S ribosomal RNA. HTM measurements were performed in diluted or centrifuged digestate samples that were enriched with S. aureus. The results indicated that it is possible to evaluate microbial load even in samples containing other organic material. The thermal analysis method has the potential to provide a low-cost monitoring option, which is simple to use and provides real-time analysis, thus improving the existing monitoring procedures in organic waste treatment facilities.

show abstract

Section: Mediasupporting

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Evaluation Of S Aureus Growth In the Nutrient Brothmentioning

confidence: 99%

See 1 more Smart Citation

Heat-Transfer Method: A Thermal Analysis Technique for the Real-Time Monitoring of Staphylococcus aureus Growth in Buffered Solutions and Digestate Samples

Betlem

Kaur

Hudson

et al. 2019

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

show abstract

“…The readout technology was soon combined with MIPs for the detection of low-molecular weight compounds [ [156] , [157] , [158] , [159] ]. The combination of thermal sensing with surface imprinting technqiues seems to work out particalurly well for the detection of macromolecular targets including proteins [ 160 ], mammalian cells [ [161] , [162] , [163] ], and microorganisms [ [164] , [165] , [166] ] in various matrices including wastewater, urine and blood. These results illustrate the importance of surface imprinting for creating synthetic receptors for macromolecular targets, which is expected to become of increasing importance in the near future for applications on the detection of e.g .…”

Section: Mips As Receptors In Sensing Devicesmentioning

confidence: 99%

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

Lowdon

Diliën

Singla

et al. 2020

Sensors and Actuators B: Chemical

166

103

View full text Add to dashboard Cite

Highlights The review aims to summarize recent commercially interesting innovations in MIP-based sensor design. In addition to sensors, commercially viable assays based on MIPs are analysed and recent advances are summarized and discussed. The review also analyses how commercial MIP companies could contribute to a next step in the commercialization of MIP-based detection systems. The review contains a critical analysis and discussion on MIP-based sensors and assay commercialization as well as an outlook/recommendation for the future.

show abstract

“…Imprinted polymers have been extensively researched for the extraction of toxins, which has also led to the application of these synthetic receptors in sensors [ 174 , 175 , 176 , 177 ]. Furthermore, bacteria detection using IPs in sensing devices has also been exploited [ 28 , 30 , 178 , 179 , 180 ]. The use of synthetic receptors as recognition element in food safety sensing exhibits the potential of becoming a fast, sensitive and cost-effective technology in contrast to the traditional analytical methods [ 181 , 182 , 183 ].…”

Section: Biological Food Hazardsmentioning

confidence: 99%

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

et al. 2021

View full text Add to dashboard Cite

Foodborne illnesses represent high costs worldwide in terms of medical care and productivity. To ensure safety along the food chain, technologies that help to monitor and improve food preservation have emerged in a multidisciplinary context. These technologies focus on the detection and/or removal of either biological (e.g., bacteria, virus, etc.) or chemical (e.g., drugs and pesticides) safety hazards. Imprinted polymers are synthetic receptors able of recognizing both chemical and biological contaminants. While numerous reviews have focused on the use of these robust materials in extraction and separation applications, little bibliography summarizes the research that has been performed on their coupling to sensing platforms for food safety. The aim of this work is therefore to fill this gap and highlight the multidisciplinary aspects involved in the application of imprinting technology in the whole value chain ranging from IP preparation to integrated sensor systems for the specific recognition and quantification of chemical and microbiological contaminants in food samples.

show abstract

Real-time analysis of microbial growth by means of the Heat-Transfer Method (HTM) using Saccharomyces cerevisiae as model organism

Cited by 16 publications

References 24 publications

Heat-Transfer Method: A Thermal Analysis Technique for the Real-Time Monitoring of Staphylococcus aureus Growth in Buffered Solutions and Digestate Samples

Heat-Transfer Method: A Thermal Analysis Technique for the Real-Time Monitoring of Staphylococcus aureus Growth in Buffered Solutions and Digestate Samples

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

Imprinted Polymers as Synthetic Receptors in Sensors for Food Safety

Contact Info

Product

Resources

About