Reaginic Antibody Production to <i>Ascaris suum</i> Allergen, ASC-1

Abstract

Rats immunized with a purified Ascaris suum allergen (Asc-1) or with its dinitrophenylated derivate (DNP-Asc-1) produced high levels of reaginic (IgE) antibodies. A second injection of antigen given 30 days later did not result in an anamnestic IgE antibody response. Immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (ATxB) rats with soluble Asc-1 or DNP-Asc-1 failed to stimulate reaginic antibody production. The administration of glutaraldehyde-polymerized antigen induced in some but not all ATxB rats, low but detectable levels of IgE antibodies. These levels increased following a second injection of nonpolymerized antigen in Al(OH)₃ gels. Priming of animals with poylmerized carrier and Bordetella pertussis did not stimulate a primary anticarrier IgE response but led to an enhanced antihapten IgE response following the administration of soluble DNP-Asc-1 in Al(OH)₃. The results are consistent with the notion that a sharply reduced but clearly functional T-derived helper cell population could be triggered by the polymerized but not by the soluble form of the immunogen.

Immunologic aspects of liposomes: presentation and processing of liposomal protein and phospholipid antigens

“…phospholipids are quite commonly found in normal 318 animal or human serum [16,174,175]. In a model sys- for experimental study [166,177].…”

Immunological tolerance of the mouse IgE system: dissociation between T cell tolerance and suppressor cell activity

“…However, the differences were never greater than 2-fold. Mouse IgG antibodies were determined by a double antibody radioimmunoassay essentially as previously described for rat IgG antibodies [36]. A standard anti-DNP antiserum was prepared by immunizing mice with 100 pg TNP-KLH in CFA twice, at 2-week intervals.…”