Immunological tolerance of the mouse IgE system: dissociation between T cell tolerance and suppressor cell activity

Abstract

Immunological tolerance was induced in CBA mice with respect to both anti-hapten and anti-carrier IgE antibody production, following pretreatment of the animals with deaggregated ovalbumin. IgG antibody production was also affected. The tolerance was antigen-specific, was stable upon adoptive transfer to irradiated syngeneic recipients, but was reversed following booster. The extent and duration of the tolerant state depended on the dosage and number of tolerogen injections. Tolerogen administered after the initiation of the primary response was without effect. The pattern and duration of this tolerance suggested that T suppressor cells were not involved. The adoptive transfer of spleen cells from tolerogen-treated donors while being themselves unresponsive, failed to interfere with the induction of an immune response in the recipient. Evidence of T suppressor cell function was found in adoptive transfers, only after prolonged pretreatment of donors with a combination of tolerogenic and immunogenic forms of the carrier. These results suggested that T cell-dependent tolerance of the IgE antibody response operates via two distinct mechanisms, of which only one is provided by suppressor cell function.

Suppressor T cell memory. II. The role of memory suppressor T cells in tolerance to human gamma globulin.

“…By contrast, the duration of complete tolerance in the intact animal is of the order of 100 d, and partial unresponsiveness may be observed for >150 d after a single injection of high-dose dHGG (26). Such differences in kinetics have hitherto provided the most cogent argument against the concept of a causal relationship between suppression and tolerance, in this (15) as well as other (27,28) experimental models. However, the apparent discrepancy can now be satisfactorily resolved in the light of evidence presented here demonstrating that Ts, like other T cell subsets, may exist in two independent physiological states, i.e., as effector cells and memory cells.…”

“…A single dose of 100 ~g dHGG was insufficient to generate detectable primary suppression; however an anamnestic secondary Ts response could be elicited by aHGG challenge after a single injection of as little as 1-10 #g of deaggregated antigen, indicating that the latter had been effective in inducing Ts memory. Thus, it would be predicted that in tolerant states not associated with significant primary suppression (17,28,31) it should nevertheless be possible to demonstrate the presence of Ts memory cells capable of abrogating the response to immunogen challenge. Experiments are in progress to test this prediction.…”

Tolerance Induction via Antigen Inhalation: Isotype Specificity, Stability, and Involvement of Suppressor T Cells

“…The recent observations of Colby and Strejan [8] on tolerance induction via intravenous administration of deaggregated OA also reflect upon this latter point. These authors have suggested the coexistence of two different T cell-dependent pathways for tolerance in duction, one of which does not involve suppressor cells.…”

Effect of Anti-DNP IgG1- and lgG2a-Secreting Hybridomas in vivo on the Development of an Anti-DNP IgE Antibody Response in Mice

“…IgE antibodies were measured by rat passive cutaneous anaphy laxis (PCA) as previously described [7]. Fourfold or greater differ ences in PCA titers between serum samples were considered statisti cally significant.…”

“…DNPj-OA, DNP^-BSA.TNP^-KLH and DNP-BP were prepared as previously decribcd [6,7]. 4-Hydroxy-3-nitro-5-iodo-phcnylacetyl (NIP) was conjugated to OA (NIP,(l-OA) from NIP-succinamide (Bioscarch Laboratories, San Rafael, Calif.) according to Wein berger et al [8].…”