Reagic serum fractions obtained by the electrophoresis-convection method

Campbell, Dan H.; Cann, John R.; Friedman, Townsend B.; Brown, Roy C.

doi:10.1016/0021-8707(50)90102-x

Cited by 35 publications

(8 citation statements)

References 4 publications

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“…Convection electrophoresis. This technique has also been ap plied to ascertain the association of sk-antibody with electropho retic serum components (11,12,13,14,15). We refer to data of Cann and Loveless (14), from which they conclude that skantibody is predominantly associated with beta globulin, on the grounds of a seeming parallelism between dilutability of fractions and their beta globulin content.…”

Section: Experimental I Electrophoresis Of Sensitizing Serumsmentioning

confidence: 99%

The Antibody Mechanism of Ragweed Allergy. Electrophoretic and Chemical Studies

Cooke¹,

Menzel²,

Myers³

1960

Int Arch Allergy Immunol

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Section: Experimental I Electrophoresis Of Sensitizing Serumsmentioning

confidence: 99%

The Antibody Mechanism of Ragweed Allergy. Electrophoretic and Chemical Studies

Cooke¹,

Menzel²,

Myers³

1960

Int Arch Allergy Immunol

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“…Newell and coworkers (19), on the other hand, previously applied the same technique and found skin-sensitizing antibody in the gamma globulin but not in the beta globulin fraction. Other work on sera containing skin-sensitizing antibody by Campbell and his coworkers (20), using the method of electrophoresis convection, indicated that reagin activity was associated with alpha and beta globulins particularly when the serum was from a patient with immediate type skin sensitivity to a single substance. However, it was shown by Campbell, et al (20) that reaginic activity occurred in all globulin fractions when the serum was from a patient who was sensitive to numerous materials.…”

Section: Discussionmentioning

confidence: 98%

“…Other work on sera containing skin-sensitizing antibody by Campbell and his coworkers (20), using the method of electrophoresis convection, indicated that reagin activity was associated with alpha and beta globulins particularly when the serum was from a patient with immediate type skin sensitivity to a single substance. However, it was shown by Campbell, et al (20) that reaginic activity occurred in all globulin fractions when the serum was from a patient who was sensitive to numerous materials. Loveless and Cann (21) used the same method to fractionate sera from insulin-sensitive and ragweed-sensitive patients.…”

Section: Discussionmentioning

confidence: 98%

Immunochemical Studies of Antitoxin Produced in Normal and Allergic Individuals Hyperimmunized With Diphtheria Toxoid

Kuhns

1954

Journal of Experimental Medicine

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It has been found that at least two varieties of diphtheria antitoxin are formed in subjects who are hyperimmunized with diphtheria toxoid (1, 2). In a large proportion of individuals the immune response is characterized by the simultaneous production of precipitating and non-precipitating antitoxins. A small proportion of subjects form only one or the other of these antitoxins.Because of its close similarity to allergic reagin of the hay-fever variety, skin-sensitizing antitoxin has been selected as a model for further investigations of the qualities peculiar to reagin. The advantages of the diphtheria system over other less well characterized antigen-antibody systems have been enumerated in an earlier publication (1). In view of studies of the egg white system by Vaughan and Kabat (3) which showed that the immediate type of skin reaction is caused by antibodies to trace substances rather than to the major antigenic component, it was important in our studies to demonstrate that skin sensitization is specifically effected by non-precipitating antitoxin. Evidence has been presented (2, 4) which indicates that immediate wheal reactions are caused by non-precipitating skin-sensitizing antitoxin, and that reactions elicited following the intradermal injection of toxoid are due to specific interaction between toxoid and this variety of antitoxin.In order to gain additional information concerning the nature and specificity of immediate wheal reactions, individual protein components of various sera were isolated with the object of analyzing their comparative skin-sensitizing abilities. It has been demonstrated (5) that the highly purified gamma globulin fraction obtained by cold ethanol precipitation (6) from a serum conraining non-precipitating, skin-sensitizing antitoxin has lost most of its ability to cause immediate wheal reaction although a corresponding antitoxin loss does not occur.

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“…of the starch block was cut into 65 to 80 segments, each s In a few experiments (R/284, R/285, R/287 and R/289) the ditch was cut out half way between the center and the cathodic end of the block. 4 The position of the ditch corresponding to the zone of application is indicated by an arrow in all the figures presented later.…”

Section: Methodsmentioning

confidence: 99%