Reagentless biosensor for hydrogen peroxide based on self-assembled films of horseradish peroxidase/laponite/chitosan and the primary investigation on the inhibitory effect by sulfide

Shan, Dan; Li, Qingbo; Ding, Shan; Xu, Jiaqi; Cosnier, Serge; Han, Xue

doi:10.1016/j.bios.2010.07.051

Cited by 49 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For instance, several works found in literature report the use of the synthetic nanoclay laponite in the preparation of chitosan-based composites for immobilization of the enzymes glucose oxidase [90] and horseradish peroxidase (HRP) [91]. In both examples, the use of the cross-linker glutaraldehyde was avoided as the enzymes were incorporated in the nanocomposite films by using step-by-step self-assembly.…”

Section: Electroanalytical Applicationsmentioning

confidence: 99%

Chitosan-Clay Bio-Nanocomposites

Darder

Ruiz‐Hitzky

2012

Environmental Silicate Nano-Biocomposites

View full text Add to dashboard Cite

The present review chapter includes an overview on the current state-of-art of chitosan-clay based bio-nanocomposites. In the same way than conventional nanocomposites, these biohybrid materials also exhibit both structural and functional properties together with biocompatibility and biodegradability, which can be of great interest for different applications. Four main areas of interest have been identified showing examples of applications as green nanocomposites, bio-nanocomposites addressed to biomedical purposes (tissue engineering and drug delivery), environmental remediation and electroanalytical devices. Finally, examples of bio-nanocomposites based on chitosan assembled to other inorganic solids have been introduced to show the versatility of this biopolymer for development of diverse type of advanced functional materials.

show abstract

Section: Electroanalytical Applicationsmentioning

confidence: 99%

Chitosan-Clay Bio-Nanocomposites

Darder

Ruiz‐Hitzky

2012

Environmental Silicate Nano-Biocomposites

View full text Add to dashboard Cite

show abstract

“…According to the existing literature, the typical concentration range of sulfide in hot spring waters and wastewaters are 9-440 μM [4]- [8] and 2-4750 μM [9]- [13], respectively. These levels of sulfide concentration can be determined by a number of analytical techniques, such as iodimetric [12], [14], spectrophotometric [15]- [22], chromatographic [23], [24], capillary electrophoretic [25], and electrochemical methods [13], [26]- [31]. Among them, electrochemical methods are of particular interest for analysis of sulfide, which is due to their prominent advantages of procedural simplicity, high sensitivity, short analysis time, capability of miniaturization, and low instrumental cost.…”

Section: H Ydrogen Sulfide (H 2 S) Is a Hazardous And Toxicmentioning

confidence: 99%

A Selective and Sensitive Sensor for Determination of Sulfide in Aquatic Environment

Safavi

Abi

2015

IEEE Sensors J.

View full text Add to dashboard Cite

A cost-effective, sensitive, and selective electrochemical sensor for the analysis of sulfide based on the high affinity of sulfide to metallic silver is proposed. To achieve high sensitivity while lowering the cost of the sensor, silver nanoparticles (Ag NPs), which possess larger metal surface area-to-volume ratio compared with bulk silver, were employed for the development of the sensor. The sensor was prepared through electrodeposition of Ag NPs on the surface of a carbon ionic liquid electrode using potentiostatic double-pulse technique, and it showed a wide linear range from 25 to 2500 µM of sulfide with a detection limit of 6.02 µM. Since the analysis of sulfide was performed at a remarkably low detection potential, the presented sensor offered high selectivity against common interferents and was successfully applied to the analysis of sulfide in a hot spring water sample.Index Terms-Carbon ionic liquid electrode, double-pulse technique, silver nanoparticles, sulfide sensor.

show abstract

“…Although there are several reports about the direct electron transfer systems using the combination of the AuNP and HRP [3,[8][9][10], the working electrode potentials were generally more negative, that is, they required a relatively high overpotential for the direct electron transfer to HRP. Recent papers using the combination of HRP and AuNP tend to ''hybrid'' approaches, that is, the nanoparticles are combined with organic/inorganic materials [11][12][13][14]. These approaches mainly aim to enhance the amount of enzyme and/or functional site, and improve the sensitivity and stability.…”

Section: Introductionmentioning

confidence: 99%

Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems

Okawa

Yokoyama

Sakai

et al. 2015

Analytical Chemistry Research

View full text Add to dashboard Cite

a b s t r a c tA reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose oxidase and urate oxidase, respectively.

show abstract

Reagentless biosensor for hydrogen peroxide based on self-assembled films of horseradish peroxidase/laponite/chitosan and the primary investigation on the inhibitory effect by sulfide

Cited by 49 publications

References 46 publications

Chitosan-Clay Bio-Nanocomposites

Chitosan-Clay Bio-Nanocomposites

A Selective and Sensitive Sensor for Determination of Sulfide in Aquatic Environment

Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems

Contact Info

Product

Resources

About