Direct electron transfer biosensor for hydrogen peroxide carrying nanocomplex composed of horseradish peroxidase and Au-nanoparticle – Characterization and application to bienzyme systems

Abstract

a b s t r a c tA reagentless electrochemical biosensor for hydrogen peroxide was fabricated. The sensor carries a monolayer of nanocomplex composed of horseradish peroxidase and Au-nanoparticle, and responds to hydrogen peroxide through the highly efficient direct electron transfer at a mild electrode potential without any soluble mediator. Formation of the nanocomplex was studied with visible spectroscopy and size exclusion chromatography. The sensor performance was analyzed based on a hydrodynamic electrochemical technique and enzyme kinetics. The sensor was applied to fabrication of sensors for glucose and uric acid through further modification of the nanocomplex-carrying electrode with the corresponding hydrogen peroxide-generating oxidases, glucose oxidase and urate oxidase, respectively.

Nanostructured Porous Electrodes by the Anodization of Gold for an Application as Scaffolds in Direct-electron-transfer-type Bioelectrocatalysis

“…[46][47][48][49][50] However, it has recently been reported that POD can work as a DET-type biocatalyst at suitable mesoporous electrodes to reduce H2O2 at a potential close to the redox potential of the protoheme (Compound I) of POD (0.7 V vs. Ag/AgCl at pH 7). [51][52][53] Such DET-type reactions of POD will draw attention of constructing bienzyme-type biosensors without any mediator. For practical purposes, it is important to easily construct scaffolds for DETtype enzymes.…”

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

“…Peroxidases have been applied in treatment of industrial wastewater, dyes decolorization, swine manure deodorization, enzyme immunoassays, analysis and diagnostic kits, enzymatic biosensors, production of functional bioactive compounds and polymer synthesis . Because of their oxidative nature, substrate specificity and capacity to catalyze in mild conditions, peroxidases can be exploited to reduce mycotoxins levels instead of conventional oxidation techniques .…”

“…11 Rice bran, an agricultural by-product from rice grain polishing consisting of germ and caryopsis outer layers, is a potential source for obtaining enzymes such lipase, 12 lipoxigenase 13 and peroxidase. 14 Peroxidases have been applied in treatment of industrial wastewater, 15,16 dyes decolorization, 17 swine manure deodorization, 18 enzyme immunoassays, 19 analysis and diagnostic kits, 20 enzymatic biosensors, 21 production of functional bioactive compounds 22 and polymer synthesis. 23 Because of their oxidative nature, substrate specificity and capacity to catalyze in mild conditions, peroxidases can be exploited to reduce mycotoxins levels instead of conventional oxidation techniques.…”

Electrodeposited nanoscale zinc oxide particles: facilitating the electron transfer of immobilised protein and biosensing

“…Owing to the notable advantages of NPs such as large surface area, high chemical stability, tunable porosity and biocompatibility, NPs have received vast attention in bioelectrochemical studies. Different kinds of materials and nanomaterials including Co 3 O 4 NPs/multi‐walled carbon nanotubes (MWCNTs)/gelatin [15], cobalt oxide [16, 17], nickel oxide NPs [18], system‐on‐chip devices [19], polymeric 3‐aminophenyl boronic acid monolayer [20], gold (Au)–MWCNT–graphene hybrid composite [21], SiO 2 coated Fe 3 O 4 /MWCNTs [22], CdS nanorods [23], carbon past electrode containing different modifiers [24], Au NPs [25], MWCNTs–Zno composite [26], poly‐3‐hydroxybutyrate membrane [27], lipopolysaccharide films [28] and nanoAu monolayer/sol–gel‐derived carbon ceramic [29] were used in the protein electrochemistry and biosensors. Nanomaterials can act as tiny conduction centres and facilitate the transfer of electrons while maintaining the biocatalytic properties of the proteins.…”