Reagentless biosensing using electrochemical impedance spectroscopy

Farace, G.; Lillie, Geoffrey C.; Hianik, Tibor; Payne, P.A.; Vadgama, Pankaj

doi:10.1016/s1567-5394(01)00166-9

Cited by 69 publications

(36 citation statements)

References 2 publications

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“…For calibration of the biosensor Rct was used as it was the most sensitive to analyte binding and is the most widely used measure for impedimetric analysis of biosensors in the field [32][33][34][35][36]. To ensure chip-to-chip variability was minimised, values were normalised to the initial baseline of the RCT measured for the fully constructed sensor.…”

Section: Discussionmentioning

confidence: 99%

The development and optimisation of nanobody based electrochemical immunosensors for IgG

Goode

Dillon

Millner

2016

Sensors and Actuators B: Chemical

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Article:Goode, J orcid.org/0000-0002-3642-1291, Dillon, G and Millner, PA orcid.org/0000-0002-4813-4302 (2016 ReuseUnless indicated otherwise, fulltext items are protected by copyright with all rights reserved. The copyright exception in section 29 of the Copyright, Designs and Patents Act 1988 allows the making of a single copy solely for the purpose of non-commercial research or private study within the limits of fair dealing. The publisher or other rights-holder may allow further reproduction and re-use of this version -refer to the White Rose Research Online record for this item. Where records identify the publisher as the copyright holder, users can verify any specific terms of use on the publisher's website. TakedownIf you consider content in White Rose Research Online to be in breach of UK law, please notify us by emailing eprints@whiterose.ac.uk including the URL of the record and the reason for the withdrawal request. This has led to the development of alternative immuno-reagents such as non-antibody binding proteins (NABPs). These are low molecular weight proteins which largely avoid the aforementioned advantages of antibodies. They are commonly produced by bacteria enabling the use of DNA technology to manipulate bioreceptors at the molecular level.Single chain VHHs (commonly known as nanobodies) are an antibody derived NABP adapted from camelid heavy chain antibodies which are the isolated binding domain. Whilst nanobodies have been used for diagnostic and therapeutic applications, they have limited demonstration in biosensors.In this study, both antibodies and nanobodies were used to construct a biosensor. In addition nanobody performance was optimised by introducing a novel peptide spacer. The role of nanobody orientation and spacing was thus investigated and spacer length was optimised, leading to an increase in the sensitivity of the biosensor. Highlights: Nanobodies have been used on impedimetric immunosensors to detect rabbit IgG  Unmodified nanobody sensors displayed a decrease in resistance upon analyte recognition.  Nanobodies were modified with a peptide spacer, effectively reversing this phenomena.  The use of a spacer also increased the sensitivity of the immunosensor.

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Section: Discussionmentioning

confidence: 99%

The development and optimisation of nanobody based electrochemical immunosensors for IgG

Goode

Dillon

Millner

2016

Sensors and Actuators B: Chemical

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“…6.7 DNA biosensors DNA biosensors for the detection of nucleic acid sequences have attracted ever increasing interests in connection with highly demanding research efforts directed to gene analysis, clinical disease diagnosis, or even forensic applications (Service, 1998, Butler, 2006, Staudt, 2001, Farace et al, 2002, Reisberga et al, 2006. Various techniques including optical, electrochemistry, surface plasmon resonance spectroscopy, and quartz crystal microbalance, etc have been well developed for DNA detection (Rosi & Mirkin, 2005, Gerion et al, 2003, Drummond et al, 2003, He et al, 2000.…”

Section: Horseradish Peroxidasementioning

confidence: 99%

Nanobiosensors for health care

Atta¹,

Galal²,

Ali³

2011

Biosensors for Health, Environment and Biosecurity

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“…Prior to ACI studies, the electrode was cycled through a single redox between À 0.1 V to À 0.9 V to À 0.1 V in order to realign the polymer chains following the binding interaction and obtain good reproducibility [27]. The impedance spectra were then measured in 0.3 M PBS (pH 7.0) at frequencies from 100 kHz down to the 10 Hz in a given open circuit voltage and at a sampling rate of 12 points per decade (AC amplitude, 5 mV).…”

Section: Electrochemical Detectionmentioning

confidence: 99%

Indicator Free DNA Hybridization Detection by Impedance Measurement Based on the DNA‐Doped Conducting Polymer Film Formed on the Carbon Nanotube Modified Electrode

Cai

et al. 2003

Electroanalysis

130

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A new biosensing strategy for direct electrochemical detection of DNA hybridization by AC impedance measurement is described. The indicator or label free approach was developed on the basis of the carbon nanotubes coupled with a conductive polymer polypyrrole (PPy). Multi-walled carbon nanotubes functionalized with carboxylic group (MWNTs-COOH) were modified on the glassy carbon electrode (GCE) and the oligonucleotide probes were doped within the electro-polymerized PPy films by serving as the sole counter anion during the growth of the conducting films. Before and after hybridization reaction with the complementary DNA sequences, a decrease of impedance values was observed as a result of the reduction of the electrode resistance. Hybridization amounts of the one-base, two-base and three-base mismatched sequences were obtained only in a 51%, 18% and 8.2% response when compared to that for the complementary matched sequence. Such unique response is attributed to the concomitant conductivity changes of the PPy-polymerized carbon nanotubes, and offers great promise for reagentless DNA hybridization analysis.

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Reagentless biosensing using electrochemical impedance spectroscopy

Cited by 69 publications

References 2 publications

The development and optimisation of nanobody based electrochemical immunosensors for IgG

The development and optimisation of nanobody based electrochemical immunosensors for IgG

Nanobiosensors for health care

Indicator Free DNA Hybridization Detection by Impedance Measurement Based on the DNA‐Doped Conducting Polymer Film Formed on the Carbon Nanotube Modified Electrode

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