Reactivity of Serum Anti-Erythrocyte Membrane Antibody in Babesia gibsoni-Infected Dogs.

Adachi, Kozo; Tateishi, Mika; Horii, Yoichiro; Nagatomo, Hiroshi; Shimizu, Takamasa; Makimura, Susumu

doi:10.1292/jvms.56.997

Cited by 13 publications

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“…This suggests the possibility that the thrombocytopenia in subclinical B. gibsoni infection is due to autoimmune responses, and the presence of anti-platelet IgG accelerates the platelet destruction in B. gibsoni infected dogs. Anemia is the most common symptom of infection with B. gibsoni [6,9,24], and is thought to be caused by mechanical destruction by the parasite, immunemediated intravascular or extravascular hemolysis, or immune-mediated extravascular hemolysis [2,3,16,17]. Oxidative damage may contribute to the destruction of erythrocytes by increasing their susceptibility to phagocytosis by macrophages [21].…”

Section: Discussionmentioning

confidence: 99%

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Matsuu

Kawabe

Koshida

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. To identify the incidence of Babesia gibsoni (B. gibsoni) in Aomori Prefecture, northeastern Japan, dogs with acute B. gibsoni infection were investigated at the Animal Teaching Hospital, Kitasato University, between April 2002 and March 2003. Eighteen dogs with acute B. gibsoni infection were recognized; they were all male dogs of the fighting dog breed Tosa. Their platelet counts were below normal and their packed cell volumes (PCVs) were at various levels. We collected blood samples from 141 Tosa dogs from Aomori Prefecture and used polymerase chain reaction assay to investigate the incidence of subclinical B. gibsoni infection. We also looked into the serological abnormalities associated with thrombocytopenia or anemia in subclinical infection. Forty-one of 87 dogs (47.1%) with histories of dog fighting, and one dog of 54 without a history of dog fighting were positive for B. gibsoni; that is, 42 of 141 dogs (29.8%) showed a positive result. The mean platelet counts of dogs with subclinical infection were significantly lower and levels of anti-platelet IgG were significantly higher than levels for dogs without infection. Anti-erythrocyte membrane IgG levels were significantly higher in dogs with subclinical infections, although mean PCVs were not significantly different. Tosa dogs from Aomori Prefecture, Japan, were highly infected with B. gibsoni subclinically and this pathogen might be successfully transmitted during dog fighting. Dogs with subclinical infections were at risk of chronic thrombocytopenia, which may be due to autoimmune mechanisms. KEY WORDS: anti-erythrocyte membrane IgG, anti-platelet IgG, Babesia gibsoni, Tosa dog.

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Section: Discussionmentioning

confidence: 99%

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Matsuu

Kawabe

Koshida

et al. 2004

The Journal of Veterinary Medical Science

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“…As regards, the serological methods, indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) for B. gibsoni parasites, are considered to be highly sensitive, but only moderately specific because of antigenic cross-reactions to B. canis [8] and normal dog erythrocytes [8, 9]. Therefore, the development of highly specific and sensitive system for the diagnosis of canine babesiosis is still awaited.…”

Section: Introductionmentioning

confidence: 99%

Canine Babesiosis in Northwestern India: Molecular Detection and Assessment of Risk Factors

Singh

et al. 2014

BioMed Research International

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In the current study, a total of 214 blood samples from dogs in and around Ludhiana, Punjab (India), suspected for canine babesiosis were examined with conventional and molecular assays. Examination of Giemsa-stained peripheral thin blood smears revealed an overall prevalence of 7.47% (16/214) for canine babesiosis encompassing 0.93% (2/214) of large Babesia and 6.54% (14/214) of Babesia gibsoni. However, molecular diagnosis revealed 15.42% (33/214) samples positive for B. gibsoni infection as evident by the presence of 671 bp amplicon. The results of multivariate analysis showed that the prevalence of B. gibsoni was associated with various risk factors, namely, age (P < 0.001; OR: 0.398; CI 95%: 0.080–1.799), sex (P = 0.022; OR: 0.849; CI 95%: 0.403–1.791), breed of host (P = 0.371; OR: 3.345; CI 95%: 1.045–10.710), and season (P = 0.230; OR: 2.143; CI 95%: 0.788–5.830). The prevalence of B. gibsoni was higher in summer as compared to winter season and in younger dogs, while breed and sex of the host were not significantly associated with the occurrence of the disease.

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“…Also, the production of antigen for these tests requires experimentally infected dogs, making production time-consuming and expensive. Moreover, the serum from B. gibsoni-infected dogs sometimes cross-reacts with erythrocytes from healthy dogs or B. canis (1,2,3,26). Therefore, the development of a high-quality system is required for the diagnosis of B. gibsoni infection.…”

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Identification and Expression of a 50-Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

et al. 2001

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Reactivity of Serum Anti-Erythrocyte Membrane Antibody in Babesia gibsoni-Infected Dogs.

Cited by 13 publications

References 0 publications

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Incidence of Canine Babesia gibsoni Infection and Subclinical Infection among Tosa dogs in Aomori Prefecture, Japan

Canine Babesiosis in Northwestern India: Molecular Detection and Assessment of Risk Factors

Identification and Expression of a 50-Kilodalton Surface Antigen of Babesia gibsoni and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

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