Reactivity of recombinant treponema pallidum (r-Tp) antigens with anti-Tp antibodies in human syphilitic sera evaluated by ELISA

Fujimura, Katsuya; Ise, Nobuyuki; Ueno, Eiichi; Hori, Takeya; Fujii, Nobuyuki; Okada, Masahisa

doi:10.1002/(sici)1098-2825(1997)11:6<315::aid-jcla1>3.0.co;2-4

Cited by 19 publications

(18 citation statements)

References 25 publications

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“…Enzyme immunoassays (EIAs) for the detection of T. pallidum antibodies have been developed to achieve this aim (Ebel et al 1998). The use of recombinant T. pallidum antigens instead of a poorly defined mixture of antigens from wild-type T. pallidum which may be mixed with rabbit testicular components, has the potential for improving the specificities of serological tests (Fujimura et al 1997, Young et al 1998.…”

supporting

confidence: 85%

Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Rodríguez

Álvarez

Fernández

et al. 2002

Mem. Inst. Oswaldo Cruz

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A recombinant-antigen enzyme immunoassay (EIA), BioSCREEN TM anti-Treponema pallidum, was compared favorably with the T. pallidum hemagglutination test, in the detection of specific antibodies in different groups of sera from patients with primary (n = 38), secondary (n = 10), early latent (n = 28) and congenital syphilis (n = 2), patients with leptospirosis ( n= 8), infectious mononucleosis (n = 7), hepatitis (n = 9), diabetes mellitus (n = 11), rheumatoid arthritis (n = 13), leprosy (n = 11), tuberculosis (n = 9), HIV/Aids ( n= 12), systemic lupus erythematosus (n = 4), rheumatic fever (n = 3), old-persons (n = 9), pregnant women (n = 29) and blood donors (n = 164

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supporting

confidence: 85%

Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Rodríguez

Álvarez

Fernández

et al. 2002

Mem. Inst. Oswaldo Cruz

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“…The Tp47 is the most abundant and highly immunogenic [8][9][10], while the 15 and 17 kDa are present in lower amount, although they both are strongly immunogenic [11,12]. Recombinant antigen-based ELISA, using these fractions, showed diagnostic performance similar or superior to the classic treponemal tests; many of them are commercially available [13][14][15][16][17][18].…”

supporting

confidence: 84%

Profile of Anti-Tp47 antibodies in patients with positive serology for syphilis analized by western blot

Miranda¹,

Sato²

2008

Braz J Infect Dis

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In Brazil, syphilis is still a great problem of public health. Serological test is essential for syphilis diagnosis and the current trend is the use of recombinant antigen in the treponemal tests, due to its confirmed higher sensibility and specificity. The purpose of the present study was to analyze the profile of anti-Tp47 antibodies in patients with positive serology for syphilis. One hundred positive sera samples were analyzed by Western Blot (WB) technique, using the recombinant antigen (rTp47). Ten of them did not present antibodies against the fraction rTp47, the results were confirmed by WB using native T. pallidum antigen. All ten samples had antibodies against the fractions Tp17 and Tp15 and presented low reactivity in VDRL, negative results or title below than 1:4. Considering that VDRL is used for therapeutic monitoring due to seroreversion of nontreponemal antibodies in response to the treatment, and that some studies reported loss of treponemal antibodies after treatment, we could speculate if these ten samples are cases of serological memory from patients previously treated for syphilis. In addition, although several features state the Tp47 fraction as one of the major antigenic components, based on our results we point out to the importance of including other antigenic proteins such as Tp17 and Tp15 in addition to Tp47 in tests for serological screening of syphilis.

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“…Serologic screening has been performed over the past years by the use of Venereal Disease Research Laboratory and hemagglutination assay (MHA-TP), with the results confirmed by the immunofluorescence method (FTA-ABS) (23,40). In recent years, however, several enzyme immunoassays based on either whole-cell lysate (8,14,24,39) or recombinant (9,12,19,48,50) treponemal antigens have been developed for the serologic screening of syphilis sera, showing sensitivities and specificities similar to those of FTA-ABS and MHA-TP. All of the serologic tests for syphilis have been shown to possibly give false results when several different conditions are present: other spirochetal diseases, autoimmune disorders, or human immunodeficiency virus infection.…”

mentioning

confidence: 99%

“…Among these polypeptides, at least five (TpN15, TpN17, TpN37, TmpA, and TpN47) proved to be of diagnostic relevance (4,23,28,31,32). While many different combinations of the above-mentioned immunogens in the recombinant form have been used in enzyme immunoassay methods (9,12,19,48,50), only a few preparations of recombinant T. pallidum polypeptides have been evaluated for the diagnosis of syphilis by WB (7,38). The use of recombinant antigens could avoid the difficulties in purifying specific T. pallidum proteins due to the complex antigenic structure of this spirochete, and it has the potential to increase the specificity of serologic investigations.…”

mentioning

confidence: 99%

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

Sambri¹,

Marangoni²,

Eyer³

et al. 2001

Clin Diagn Lab Immunol

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Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially cross-reactive) were tested to evaluate the diagnostic performance of recombinant Western blotting (recWB) in comparison with in-house whole-cell lysate antigen-based immunoblotting (wclWB) and T. pallidum hemagglutination (MHA-TP) for the laboratory diagnosis of syphilis. None of the serum specimens from blood donors or from potential cross-reactors gave a positive result when evaluated by recWB, wclWB, or MHA-TP. The evaluation of the immunoglobulin G immune response by recWB in sera from patients with different stages of syphilis showed that rTmpA was the most frequently identified antigen (95%), whereas only 41% of the specimens were reactive to rTpN37. The remaining recombinant polypeptides were recognized as follows: rTpN47, 92.5%; rTpN17, 89.5%; and rTpN15, 67.5%. The agreement between recWB and MHA-TP was 95.0% (100% with sera from patients with latent and late disease), and the concordance between wclWB and MHA-TP was 92.0%. The overall concordance between recWB and wclWB was 97.5% (100% with sera from patients with secondary and late syphilis and 94.6 and 98.6% with sera from patients with primary and latent syphilis, respectively). The overall sensitivity of recWB was 98.8% and the specificity was 97.1% with MHA-TP as the reference method. These values for sensitivity and specificity were slightly superior to those calculated for wclWB (sensitivity, 97.1%, and specificity, 96.1%). With wclWB as the standard test, the sensitivity and specificity of recWB were 98.9 and 99.3%, respectively. These findings suggest that the five recombinant polypeptides used in this study could be used as substitutes for the whole-cell lysate T. pallidum antigens and that this newly developed recWB test is a good, easy-to-use confirmatory method for the detection of syphilis antibodies in serum.

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Reactivity of recombinant treponema pallidum (r-Tp) antigens with anti-Tp antibodies in human syphilitic sera evaluated by ELISA

Cited by 19 publications

References 25 publications

Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Profile of Anti-Tp47 antibodies in patients with positive serology for syphilis analized by western blot

Western Immunoblotting with FiveTreponema pallidumRecombinant Antigens for Serologic Diagnosis of Syphilis

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