Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Rodríguez, Islay; Álvarez, Elvio L; Fernández, Carmen; Miranda, Alina

doi:10.1590/s0074-02762002000300012

Cited by 21 publications

(16 citation statements)

References 7 publications

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“…The sensitivities of the tests varied from 48.5 to 100%, according to the type of ELISA, with the phase of the disease, and with the disease prevalence (1,6,7,12,14,16). In this study, the ELISA showed a sensitivity that was similar to those of the tests generally used for the laboratory diagnosis of syphilis.…”

Section: Discussionsupporting

confidence: 54%

Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum

et al. 2003

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In the present study, the performance of an enzyme-linked immunosorbent assay (ELISA) technique (Eti-syphilis-G and Eti-syphilis-M; DiaSorin) for detection of Treponema pallidum immunoglobulin M (IgM) and IgG antibodies for the laboratory diagnosis of syphilis was evaluated. Four hundred forty-one samples were studied. The sensitivity and specificity of the ELISA were 100 and 93%, respectively, compared with the results of a microhemagglutination assay for Treponema pallidum (MHA-TP) and 99.4 and 100%, respectively, compared with the results of the fluorescent treponemal antibody absorption (FTA-Abs) test. The results of the ELISA technique were concordant with those of MHA-TP for 98% of the samples tested, while the rate of concordance with the FTA-Abs test was 99.5%. The sensitivities of the rapid plasma reagin (RPR) test, MHA-TP, and the ELISA in the different phases of syphilis compared with the results of the FTA-Abs test were 92, 88, and 100%, respectively, for patients with primary syphilis; 100% for all tests evaluated for patients with secondary syphilis; 97.2, 99.4, and 100%, respectively, for patients with latent syphilis; and 57.9, 92.6, and 97.9%, respectively, for patients with past treated syphilis. The RPR test was reactive with 12 samples that were negative by all the specific tests. IgM antibodies were most frequently detected by the ELISA for IgM antibodies (32.8%) than by the FTA-Abs for IgM antibodies (28.4%). Detection of these antibodies by the FTA-Abs test and the ELISA for IgM antibodies decreased with the stage of disease (72 and 88%, respectively, for patients with primary syphilis to 17 and 19%, respectively, for patients with early latent syphilis). The high sensitivity and specificity of this ELISA technique during all stages of syphilis, together with the fact that it is a simple, objective, and easily automated method, lead us to believe that it could be used as a screening test for syphilis.

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Section: Discussionsupporting

confidence: 54%

Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum

et al. 2003

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“…To screen for syphilis, Treponema pallidum haemagglutination tests (TPHA) with a sensitivity of 98.7% and a specificity of 95.4% (Oxoid, Basingstoke, UK) were used. In an independent evaluation, the sensitivity was 92% for primary syphilis (35/38), 100% for secondary syphilis (10/10), early latent syphilis (28/28) and congenital syphilis (2/2), respectively, while specificity was 100% with no cross‐reactions with samples from patients with leptospirosis (8), infectious mononucleosis (7), hepatitis (9), diabetes mellitus (11), rheumatoid arthritis (13), leprosy (11), tuberculosis (9), HIV/AIDS (12), systemic lupus erythematosus (4), rheumatic fever (3), as well as from elderly patients (9), pregnant women (29) and blood donors (164) (Rodriguez et al. 2002).…”

Section: Methodsmentioning

confidence: 99%

Serological survey of HIV and syphilis in pregnant women in Madagascar

Frickmann

Schwarz

Girmann

et al. 2012

Tropical Med Int Health

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Abstractobjectives Peripartal transmission of human immunodeficiency virus (HIV) and Treponema pallidum, the causative agent of syphilis, leads to severe consequences for newborns. Preventive measures require awareness of the maternal infection. Although HIV and syphilis testing in Madagascar could be theoretically carried out within the framework of the national pregnancy follow-up scheme, the required test kits are rarely available at peripheral health centres. In this study, we screened blood samples of pregnant Madagascan women for HIV and syphilis seroprevalence to estimate the demand for systemic screening in pregnancy.methods Retrospective anonymous serological analysis for HIV and syphilis was performed in plasma samples from 1232 pregnant women that were taken between May and July 2010 in Ambositra, Ifanadiana, Manakara, Mananjary, Moramanga and Tsiroanomandidy (Madagascar) during pregnancy follow-up. Screening was based on Treponema pallidum haemagglutination tests for syphilis and rapid tests for HIV, with confirmation of positive screening results on line assays.results Out of 1232 pregnant women, none were seropositive for HIV and 37 (3%) were seropositive for Treponema pallidum.conclusions Our findings are in line with previous studies that describe considerable syphilis prevalence in the rural Madagascan population. The results suggest a need for screening to prevent peripartal Treponema pallidum transmission, while HIV is still rare. If they are known, Treponema pallidum infections can be easily, safely and inexpensively treated even in pregnancy to reduce the risk of transmission.

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“…Recently, several enzyme immunoassays, some of which are based on specific T. pallidum recombinant antigen,[10–12] have been developed and evaluated as Treponemal test for syphilis. [313–17] The advantages of ELISAs are the capacity to process large number of samples and ability to have a print out of the objective spectrophotometric readings, while the TPHA, TPPA ( Treponema pallidum particle agglutination assays), and FTA-ABS (Fluorescent Treponemal Antibody absorption) are subjective assays[218] because they rely on the skill and training of the individual reader which is variable and depends upon the person reading the test.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of Treponemal and non-Treponemal test for screening of blood donated at a blood center

Naidu¹,

Bharucha²,

Sonawane³

et al. 2012

Asian J Transfus Sci

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The non-Treponemal tests such as Rapid Plasma Reagin test (RPR) or the Venereal Disease Reference Laboratory test are the most commonly used test for screening of syphilis in the blood centers in India. Now, with the availability of Enzyme-linked immunosorbent assay (ELISA) and Immunochromatographic assays in the market, we decided to evaluate these assays in comparison with Treponema pallidum Haemagglutination Assay (TPHA) which was considered as a gold standard for this study. A total of 8 685 samples of voluntary blood donors were tested on Trepolisa 3.0 and then the initially reactive samples were retested in duplicate on the same assay as well as on Omega Pathozyme, RPR, RAPHA (Rapid Anti-Treponema pallidum Assay), and TPHA. Of the 158 initially reactive samples, 104 were repeatedly reactive on the same assay, 85 were reactive with RPR, 77 were reactive with RAPHA, 60 were reactive on Omega, and 53 were confirmed reactive on TPHA. 48 (56.4%) of the results on RPR were biological false positive, while 21.9% of results were false negative on RPR. We evaluated that Omega Pathozyme was quite in agreement with TPHA as compared with Trepolisa 3.0, RAPHA, and RPR. We concluded that Omega Pathozyme (ELISA) can be considered as a suitable test for screening of syphilis in a blood center.

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Comparison of a Recombinant-antigen Enzyme Immunoassay with Treponema pallidum Hemagglutination Test for Serological Confirmation of Syphilis

Cited by 21 publications

References 7 publications

Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum

Evaluation of an Enzyme Immunoassay Technique for Detection of Antibodies against Treponema pallidum

Serological survey of HIV and syphilis in pregnant women in Madagascar

Comparative study of Treponemal and non-Treponemal test for screening of blood donated at a blood center

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