Reactivity of isolated bovine mesenteric and hepatic veins to vasoactive agents and specific antigen

1980

1This paper describes a preliminary pharmacological study of the isolated helical strip of bovine bronchial artery under isotonic conditions in Krebs-Henseleit solution. 2 The tissue contracted to histamine and 5-hydroxytryptamine (5-HT), actions which were selectively antagonized by mepyramine and methysergide respectively. Histamine was not inhibited by metiamide and 5-HT was not affected by morphine.3 Slow reacting substance of anaphylaxis (SRS-A), prostaglandin F2. (PGF2X) and PGE2 were spasmogenic, whereas PGE1 caused relaxation. No potential antagonists of these agents were tested. 4 Carbachol, at all concentrations, caused contractions of the bronchial artery which were completely, irreversibly blocked by atropine. 5 The spasmogenic action of phenylephrine was selectively blocked by dibenamine. Vessel strips which were partially contracted to histamine, relaxed to isoprenaline and salbutamol. The action of isoprenaline was inhibited similarly by either propranolol or practolol. The evidence therefore suggests the presence of functional a, I, and #2-adrenoceptors.

Section: Introductionmentioning

confidence: 79%

Preliminary Pharmacological Characterization of the Bovine Isolated Bronchial Artery Strip: A New Preparation

Arowolo

1980

“…Since then, antigen-induced smooth muscle contraction in vitro has become a standard technique for demonstrating anaphylactic hypersensitivity. Subsequently this 'Schultz-Dale' effect has been described in the oesophagus (Califano & Scapagnini, 1968) and ileum (Dale, 1965;Dale & Okpako, 1969;Okpako, 1970) of guinea-pig; jejunum (Aitken, Deline & Eyre, 1975), pulmonary (Eyre, 1971;Aitken et al, 1975), mesenteric and hepatic (Holroyde & Eyre, 1975) blood vessels of calf. However, Hirata & Cambell (1961) failed to demonstrate a Schultz-Dale reaction in the intestine of passively sensitized adult domestic fowl.…”

Section: Introductionmentioning

confidence: 94%

The Pharmacology of Anaphylaxis in the Chicken Intestine

Chand

1976

“…When all these sites are occupied, the drug may activate a-adrenoceptors and induce contractile responses. This would indicate relatively poor specificity of isoprenaline on certain blood vessels (Eyre, 1971b;Holroyde & Eyre, 1975; Elmes & Eyre, unpublished) and/or may suggest relatively greater intrinsic activity at a-adrenoceptors (Ariens, 1954) in this system.…”

Section: Discussionmentioning

confidence: 98%

“…pulmonary vessels of rabbit (Lecomnte, 1958), calf (Eyre, 1970;1971a;Eyre & Deline, 1971a,b;, horse (Eyre, 1972;Burka et al, 1976); and the hepatic (Holroyde & Eyre, 1975) (Chand & Eyre, 1976).…”

Section: Discussionmentioning

confidence: 99%

“…This immunopharmacological reaction has subsequently been demonstrated in numerous tissues: the guinea-pig ileum (Dale & Okpako, 1969;Dale & Zilleti, 1970;Cirstea, 1970;Okpako, 1970) and trachea (S6renby, 1975); rat uterus (Kellaway, 1930); human tracheal and bronchial strips (Schild, Hawkins, Mongar & Herxheimer, 1951;Schild, 1956); pulmonary artery of the rabbit (Lecomte, 1958) and calf (Eyre, 1970;; calf pulmonary veins (Eyre, 1970;1971a;Eyre & Deline, 1971a, b;, hepatic veins (Holroyde & Eyre, 1975) and digital veins (Elmes & Eyre, unpublished); pulmonary vein of the horse (Eyre, 1972;Burka, Deline, Holroyde & Eyre, 1976) and recently several gastrointestinal tissues ofthe domestic fowl (Chand & Eyre, 1976). Surprisingly, sheep pulmonary vein has been shown to relax to antigen (Eyre, 1975).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Anaphylactic Contraction of Pulmonary Blood Vessels of Chicken

Chand

1977

1Isolated pulmonary arterial and vein strips from sensitized or non-sensitized chickens exhibited dose-dependent contractions to adrenaline>, noradrenaline>, 5-hydroxytryptamine>, histamine>, dopamine. Individual variability in the responsiveness of the vessels to agonists was marked. In general veins were 2 to 25 times more sensitive to agonists than arterial strips. 2 Isoprenaline (a relatively specific ,B-adrenoceptor agonist) induced relaxations of the submaximally contracted pulmonary vein and arteries at low doses and contractions at high concentrations. 3 Contractile responses to acetylcholine or carbachol were not regularly recorded; only 50% of the vessels reacted to cholinoceptor agonists over a wide threshold dose range. 4 Chicken pulmonary vessels were found relatively insensitive to bradykinin. 5Effects of prostaglandins were variable. Prostaglandin Fm induced dose-related contractions of the vein and arterial strips; prostaglandins E1 and E2 at low doses relaxed partially contracted pulmonary artery irrespective of the spasmogen used and further increase in doses induced either no effect or contractions. Prostaglandin E1 induced marked and rapid contractions of the vein.Prostaglandin E2 induced relaxations of the prostaglandin Fm-contracted vein only, but produced no effect or slight contractions of the veins partially contracted to other spasmogens. 6 Pulmonary arterial and vein strips obtained from chickens sensitized to horse plasma exhibited Schultz-Dale contractions of variable magnitude and duration to specific antigenic challenge only. In many vessels, antigen-induced contractions were associated with marked increase in spontaneous activity. 7 The importance of the Schultz-Dale reaction in avian pulmonary vessels is discussed in relation to the right heart dilatation associated with anaphylaxis in the chicken.