Reactivity of European and American Isolates of Borrelia burgdorferi with Different Monoclonal Antibodies by Means of a Microimmunoblot Technique

Stanek, Gerold; Jurkowitsch, Brigitte; Köchl, Cornelia; Burger, Ingrid; Khanakha, Gelas

doi:10.1016/s0934-8840(11)80043-5

“…The precise antigenic properties were quite different from that of strains B31 and P/Bi and other American and European strains (14). The antigenic differences between the American and European borrelial strains have already been shown by other investigators (4,(17)(18)(19). Particularly, a serotyping system based on the reactivity of the Borrelia Osp A with two MAb, H5332 (2) and H3TS (4), was proposed by Barbour (4): serogroup I Hokkaido, American, and European strains of B. burgdorferi and found that antigenic charactrization and serotyping among Japanese strains are necessary for the achievement of serodiagnosis of Lyme disease and for the development of a Lyme borreliosis vaccine in Japan.…”

supporting

confidence: 67%

Immunological Properties of Borrelia burgdorferi Isolated from the Ixodes ovatus in Shizuoka, Japan

Masuzawa

¹

,

Okada

²

,

Beppu

³

et al. 1991

Microbiology and Immunology

View full text Add to dashboard Cite

Three strains of spirochetes (IKA1 to 3) were isolated from the midgut of Ixodes ovatus collected in the Ikawa region of the northern part of Shizuoka, Japan. These isolates had eight flagella, and their size and other morphological features were similar to Borrelia burgdorferi. They showed similar motility and reacted with monoclonal antibody (MAb) H9724 against borrelial flagella and with MAb H5332 against the outer surface protein A. These strains showed similar SDS-PAGE profiles to that of B. burgdorferi strain B31 and P/Bi isolated in the U.S.A. and Europe, respectively. Immunoblot with Lyme disease patient serum showed positive reactions with the flagella (41 Kilodalton, kDa), protein C (20 to 22 kDa), and outer surface protein A (29 kDa) of the isolates. Immunological properties, morphological characteristics, and epidemiological features revealed that these isolates were B. burgdorferi.

show abstract

“…The precise antigenic properties were quite different from that of strains B31 and P/Bi and other American and European strains (14). The antigenic differences between the American and European borrelial strains have already been shown by other investigators (4,(17)(18)(19). Particularly, a serotyping system based on the reactivity of the Borrelia Osp A with two MAb, H5332 (2) and H3TS (4), was proposed by Barbour (4) Hokkaido, American, and European strains of B. burgdorferi and found that antigenic charactrization and serotyping among Japanese strains are necessary for the achievement of serodiagnosis of Lyme disease and for the development of a Lyme borreliosis vaccine in Japan.…”

Section: Lyme Disease or Lyme Borreliosismentioning

confidence: 63%

Antigenic properties of Borrelia burgdorferi isolated from Ixodes ovatus and Ixodes persulcatus in Hokkaido, Japan

Masuzawa

¹

,

Okada

²

,

Yanagihara

³

et al. 1991

View full text Add to dashboard Cite

Three strains of spirochetes (IKA1 to 3) were isolated from the midgut of Ixodes ovatus collected in the Ikawa region of the northern part of Shizuoka, Japan. These isolates had eight flagella, and their size and other morphological features were similar to Borrelia burgdorferi. They showed similar motility and reacted with monoclonal antibody (MAb) H9724 against borrelial flagella and with MAb H5332 against the outer surface protein A. These strains showed similar SDS-PAGE profiles to that of B. burgdorferi strain B31 and P/Bi isolated in the U.S.A. and Europe, respectively. Immunoblot with Lyme disease patient serum showed positive reactions with the flagella (41 Kilodalton, kDa), protein C (20 to 22 kDa), and outer surface protein A (29 kDa) of the isolates. Immunological properties, morphological characteristics, and epidemiological features revealed that these isolates were B. burgdorferi.

show abstract

“…Western blot (immunoblot) [22] studies were performed in 27 mor phea patients. As controls, we investigated 10 patients with ACA.…”

Section: Western Blotsmentioning

confidence: 99%

<i>Borrelia burgdorfer</i><i>i</i> and Different Types of Morphea

Aberer¹,

Klade²,

Stanek

³

et al. 1991

View full text Add to dashboard Cite

Thirty patients with different clinical manifestations of morphea (circumscribed scleroderma) were investigated for serum antibodies against Borrelia burgdorferi determined by ELISA and Western blot analysis. Forty-six percent of the patients were seropositive. Western blots confirmed the ELISA results in 10 of 25 patients (40%), showing a reactivity pattern which can be seen in the course of Lyme borreliosis. In some cases the outcome after antibiotic treatment suggests a direct correlation between the further development of skin lesions and Borrelia infection. Because of these findings we suggest some morphea types to be possibly due to a B. burgdorferi infection.

show abstract

Reactivity of European and American Isolates of Borrelia burgdorferi with Different Monoclonal Antibodies by Means of a Microimmunoblot Technique

Cited by 24 publications

References 11 publications

Immunological Properties of Borrelia burgdorferi Isolated from the Ixodes ovatus in Shizuoka, Japan

Immunological Properties of Borrelia burgdorferi Isolated from the Ixodes ovatus in Shizuoka, Japan

Antigenic properties of Borrelia burgdorferi isolated from Ixodes ovatus and Ixodes persulcatus in Hokkaido, Japan

<i>Borrelia burgdorfer</i><i>i</i> and Different Types of Morphea

Contact Info

Product

Resources

About