Immunological Properties of Borrelia burgdorferi Isolated from the Ixodes ovatus in Shizuoka, Japan

et al. 1996

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Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I. ovatus (adult) were the common ticks on sika deer. Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature.

Section: Discussionmentioning

confidence: 81%

Borrelia burgdorferi Sensu Lato in an Endemic Environment: Wild Sika Deer (Cervus nippon yesoensis) with Infected Ticks and Antibodies

Isogai

et al. 1996

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“…Fresh isolates were obtained from culture of midgut from adult ticks of I. ovatus collected by flagging in the Ikawa region in the northern part of Shizuoka. Methods for tick collection and isolation of Borrelia were described previously (19). The strains were cultivated at 33 C in Barbour-Stoenner-Kelly II (BSK II) medium (3).…”

Section: Methodsmentioning

confidence: 99%

“…burgdorferi sensu lato was isolated from ixodid ticks, I. persulcatus (20,24), I. ovatus (19,20,25), other ixodid ticks (28), wood mouse (Apodemus speciosus ainu; 24), long-tailed shrew (Sorex unguiculatus; 30) and patients with erythema migrans (EM; 26) in Japan. Interestingly, isolates from I. ovatus were homologous with respect to the molecular weight of Osp A (27), and RFLP in 23S rRNA gene (31).…”

mentioning

confidence: 99%

Infectivity and Early Antibody Response to Borrelia burgdorferi Sensu Lato Isolated in Japan in Outbred Mice

Kurita

Kawabata

et al. 1994

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“…Virulence and infectivity of B. japonica to human is not determined yet. Despite of many cases suffering I. ovatus bite, only one suspected case has been reported in Japan (18). Susceptibility of clinical isolates from EM (JEM1-JEM8) to three antibiotics, amoxicillin, erythromycin and minocycline, by which efficacy against four Japanese isolates, 12-92 vi, HP1 vi, NP81 vi, and NO129-6E, were proven as mentioned above, was examined.…”

mentioning

confidence: 99%

“…Borrelia was isolated from ixodid ticks, I. persulcatus (19,21), I. ovatus (18,21), other ixodid ticks (22), wood mouse (Apodemus speciosus ainu; 23), long-tailed shrew (Sorex unguiculatus; 24) and skin lesion of erythema migrans (EM) of patients (20). B. japonica, a new species, isolated from I. ovatus and long-tailed shrew was described on the basis of genomic DN A hybridization analysis and restriction fragment length polymorphism analyses of the flagellin structural genes and the 16S rRNA genes (17).…”

mentioning

confidence: 99%

In Vitro Antibiotic Susceptibilities of Borrelia Isolates from Erythema Migrans Lesion of Lyme Disease Patients in Japan

Yamada

Kawabata

et al. 1994

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Antibiotic susceptibilities of twelve borrelial isolates from skin of patients with erythema migrans (EM) and ticks (Ixodes persulcatus and I. ovatus) in Japan were examined by in vitro microdilution MIC method and macrodilution MBC method. Nine EM isolates and 3 tick isolates were susceptible to amoxicillin, erythromycin, and minocycline. MICs for Japanese isolates were 0.038–0.30 μg/ml, < 0.012 μg/ml, and < 0.012–0.05 μg/ml, respectively. MBCs were as follows: 0.038–0.88 μg/ml, < 0.012–0.10 μg/ml, and <0.025–0.78 μg/ml, respectively. These antibiotics could be recommended for treatment of patients in early stage of Lyme disease in Japan.