Reactive Oxygen Species Mediate Isoalantolactone-Induced Apoptosis in Human Prostate Cancer Cells

Rasul, Azhar; Di, Jun; Millimouno, Faya Martin; Malhi, Mahadev; Tsuji, Ichiro; Ali, Muhammad; Li, Jiang; Li, Yongming

doi:10.3390/molecules18089382

Cited by 60 publications

(43 citation statements)

References 39 publications

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“…Prostate cancer (PC), an androgen-sensitive cancer, is one of the most common malignancies among males in the United States and western world (1,2). Recently, 233,000 new PC cases are reported by American Cancer Society statistics in 2014 and it is estimated that about 29,480 patients are expected to die from it (3).…”

Section: Introductionmentioning

confidence: 99%

Effects of Thymoquinone on IL-6 Gene Expression and Some Cellular Signaling Pathways in Prostate Cancer PC3 Cells

Ranjbari

Heidarian

Ghatreh‐Samani

2017

Jundishapur J Nat Pharm Prod

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Section: Introductionmentioning

confidence: 99%

Effects of Thymoquinone on IL-6 Gene Expression and Some Cellular Signaling Pathways in Prostate Cancer PC3 Cells

Ranjbari

Heidarian

Ghatreh‐Samani

2017

Jundishapur J Nat Pharm Prod

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“…The regulation of apoptosis is, therefore, the most important in the treatment of cancer (Fulda, 2010;Lawen, 2003;Reed, 2002). Accumulated evidences indicated that the most of chemotherapeutic agents halt tumor cells proliferation via induction of apoptosis (Rasul et al, 2013a;Rasul et al, 2013b). TBMS1-induced cell death could be associated with apoptosis and necrosis (Kanduc et al, 2002;Kerr et al, 1972).…”

Section: Resultsmentioning

confidence: 99%

Tubeimoside-1 upregulates p21 expression and induces apoptosis and G2/M phase cell cycle arrest in human bladder cancer T24 cells

Rasul

Shen

Wang

et al. 2014

Bangladesh J Pharmacol

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Tubeimoside-1 (TBMS1) is a triterpenoid saponin with potent anticancer properties. In this study, for the first, we examined the anti-proliferative effects of TBMS1 in human bladder cancer T24 cells and its ability to induce apoptosis and cell cycle arrest. Our results demonstrated that TBMS1 decreased the cell viability of bladder cancer T24 cells in a dose-dependent manner. Flow cytometric analysis showed that TBMS1 significantly triggered apoptosis in T24 cells and arrested cell cycle at G2/M phase in a dosedependent manner. Further characterization demonstrated that TBMS1-induced apoptosis is associated with dissipation in mitochondrial membrane potential (ΔΨm), down-regulation of Bcl-2, and up-regulation of Bax and p21 in TBMS1-treated T24 cells. These in vitro results suggested that TBMS1 is an effective anti-bladder cancer natural compound that worth further mechanistic and therapeutic studies in human bladder cancer. Article InfoReceived:12 which is regulated by down-regulation of Bcl-2 and upregulation of Bax and p21. This study will pave the way for further in vivo studies of this potent anti-bladder cancer compounds. Material and Methods Chemicals and reagents:Cell culture medium reagents and MTT [3′-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide], propidium iodide (PI), and dimethyl sulfoxide (DMSO) were purchased from Sigma. Fetal bovine serum (FBS) was purchased from the Hangzhou Sijiqing Biological Engineering Materials Co., Ltd. Annexin V-FITC apoptosis detection kit was purchased from Beyotime Institute of Biotechnology Shanghai, China. Rabbit polyclonal anti-human Bcl-2, Bax, and p21 antibodies were purchased from Wuhan Boster Biological Technology Co., Ltd. β-actin, antimouse and anti-rabbit antibodies were purchased from Santa Cruz Biotechnology. Ponceou and cell lysis buffer for Western and IP were purchased from Bio SS Beijing. Rhodamine 123 was purchased from Eugene Co. (Oregon, U.S.A.).Cell culture: Human bladder cancer cell line (T24) was purchased from the Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Sciences (Shanghai, China). The cells were cultured in DMEM supplemented with 10% FBS and 100 unit of Penicillin at 37℃ in a CO2 incubator with 5% CO2, 95% air and 100% humidity. Cells were plated in 10 cm culture dish and allowed to grow to approximately 70% confluence before experimentation.

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“…It also induced apoptosis in both androgen-sensitive (LNCaP) as well as androgenindependent (PC3 and DU-145) prostate cancer cells with the generation of ROS and dissipation of MMP (Dcm; ref. 136). Alantolactone induced apoptosis accompanied by ROS generation and mitochondrial transmembrane potential dissipation (49,137).…”

Section: Targeting Cancer Cells By Ros-mediated Apoptosis Pathwaymentioning

confidence: 99%

Targeting Apoptosis Pathways in Cancer and Perspectives with Natural Compounds from Mother Nature

Millimouno

Dong

Liu

et al. 2014

Cancer Prevention Research

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Although the incidences are increasing day after day, scientists and researchers taken individually or by research group are trying to fight against cancer by several ways and also by different approaches and techniques. Sesquiterpenes, flavonoids, alkaloids, diterpenoids, and polyphenolic represent a large and diverse group of naturally occurring compounds found in a variety of fruits, vegetables, and medicinal plants with various anticancer properties. In this review, our aim is to give our perspective on the current status of the natural compounds belonging to these groups and discuss their natural sources, their anticancer activity, their molecular targets, and their mechanism of actions with specific emphasis on apoptosis pathways, which may help the further design and conduct of preclinical and clinical trials. Unlike pharmaceutical drugs, the selected natural compounds induce apoptosis by targeting multiple cellular signaling pathways including transcription factors, growth factors, tumor cell survival factors, inflammatory cytokines, protein kinases, and angiogenesis that are frequently deregulated in cancers and suggest that their simultaneous targeting by these compounds could result in efficacious and selective killing of cancer cells. This review suggests that they provide a novel opportunity for treatment of cancer, but clinical trials are still required to further validate them in cancer chemotherapy. Cancer Prev Res; 7(11);

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Reactive Oxygen Species Mediate Isoalantolactone-Induced Apoptosis in Human Prostate Cancer Cells

Cited by 60 publications

References 39 publications

Effects of Thymoquinone on IL-6 Gene Expression and Some Cellular Signaling Pathways in Prostate Cancer PC3 Cells

Effects of Thymoquinone on IL-6 Gene Expression and Some Cellular Signaling Pathways in Prostate Cancer PC3 Cells

Tubeimoside-1 upregulates p21 expression and induces apoptosis and G2/M phase cell cycle arrest in human bladder cancer T24 cells

Targeting Apoptosis Pathways in Cancer and Perspectives with Natural Compounds from Mother Nature

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