Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Kyseláková, Helena; Sedlářová, Michaela; Kubala, Martin; Nožková, Vladimíra; Piterková, Jana; Luhová, Lenka; Novák, Ondřej; Ilı́k, Petr

doi:10.17221/51/2012-pps

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…Numerous studies have demonstrated that plants are able to protect against abiotic stress (Gill and Tuteja, 2010 ; Li et al, 2011 ) or biotic stress (Gonçalves et al, 2013 ; Kyseláková et al, 2013 ) by increasing the activities of antioxidant enzymes such as SOD, CAT, and POD. In the present study, we found that drought stress significantly increased the activities of SOD, POD, and CAT in both the healthy and diseased plantlets, but single virus infection increased slightly the activity of antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 99%

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

Cui

Hao

et al. 2016

Front. Physiol.

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Stresses caused by viral diseases and drought have long threatened sustainable production of grapevine. These two stresses frequently occur simultaneously in many of grapevine growing regions of the world. We studied responses of in vitro-grown plantlets (Vitis vinifera) to Grapevine leafroll associated virus-3 (GLRaV-3) and PEG-induced drought stress. Results showed that stress induced by either virus infection or drought had negative effects on vegetative growth, caused significant decreases and increases in total soluble protein and free proline, respectively, induced obvious cell membrane damage and cell death, and markedly increased accumulations of O2·- and H2O2. Co-stress by virus and drought had much severer effects than single stress on the said parameters. Virus infection alone did not cause significant alternations in activities of POD, ROS, and SOD, and contents of MDA, which, however, markedly increased in the plantlets when grown under single drought stress and co-stress by the virus and drought. Levels of ABA increased, while those of IAA decreased in the plantlets stressed by virus infection or drought. Simultaneous stresses by the virus and drought had co-effects on the levels of ABA and IAA. Up-regulation of expressions of ABA biosynthesis genes and down-regulation of expressions of IAA biosynthesis genes were responsible for the alternations of ABA and IAA levels induced by either the virus infection or drought stress and co-stress by them. Experimental strategies established in the present study using in vitro system facilitate investigations on ‘pure’ biotic and abiotic stress on plants. The results obtained here provide new insights into adverse effects of stress induced by virus and drought, in single and particularly their combination, on plants, and allow us to re-orientate agricultural managements toward sustainable development of the agriculture.

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Section: Discussionmentioning

confidence: 99%

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

Cui

Hao

et al. 2016

Front. Physiol.

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“…The stock solution was diluted to 1 mM in 10 mM TRIS-HCl buffer. Later, the AgNPs and AgBulk diluted in distilled water were added to the solution (Baccelli et al, 2014;Grunberg and Taleisnik, 2015;Kyselakova et al, 2013;Zabala et al, 2015).…”

Section: Reactive Oxygen Species Analysismentioning

confidence: 99%

Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

Falco

Scherer

Oliveira

et al. 2020

Science of The Total Environment

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Although a positive correlation among chlorotic symptom development, the number of viral copies and the generation of hydrogen peroxide, reduced glutathione and pyridine nucleotides has been observed in SuCMoV‐infected sunflower , it is still not clear whether ROS causes the symptoms or is a result of symptom development during viral infection. It has been reported that low ROS concentrations lead to signal transduction and acclimation/defense responses , whereas high ROS levels lead to oxidation damage to lipids, DNA and proteins, and ultimately cell death . Therefore, monitoring ROS generation during symptom development is helpful in elucidating the mechanism of viral infection and symptom development based on ROS.…”

Section: Introductionmentioning

confidence: 99%

The detection of hydrogen peroxide involved in plant virus infection by fluorescence spectroscopy

Lei

Qiu

et al. 2016

Luminescence

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The production of reactive oxygen species (ROS) forms part of the defense reaction of plants against invading pathogens. ROS have multifaceted signaling functions in mediating the establishment of multiple responses. To verify whether hydrogen peroxide (H2 O2 ) contributes to plant virus infection and the development of induced symptoms, we used fluorescence to monitor the generation of H2 O2 and confocal laser scanning microscopy (CLSM) to investigate the subcellular distribution of H2 O2 in leaves. In this study, the M strain of Cucumber mosaic virus (M-CMV) induced heavy chlorotic symptoms in Nicotiana tabacum cv. white burley during systemic infection. Compared with mock-inoculated leaves, H2 O2 accumulation in inoculated leaves increased after inoculation, then decreased after 4 days. For systemically infected leaves that showed chlorotic symptoms, H2 O2 accumulation was always higher than in healthy leaves. Subcellular H2 O2 localization observed using CLSM showed that H2 O2 in inoculated leaves was generated mainly in the chloroplasts and cell wall, whereas in systemically infected leaves H2 O2 was generated mainly in the cytosol. The levels of coat protein in inoculated and systemically infected leaves might be associated with changes in the level of H2 O2 and symptom development. Further research is needed to elucidate the generation mechanism and the relationship between coat protein and oxidative stress during infection and symptom development. Copyright © 2016 John Wiley & Sons, Ltd.

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Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Cited by 7 publications

References 62 publications

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

Responses of In vitro-Grown Plantlets (Vitis vinifera) to Grapevine leafroll-Associated Virus-3 and PEG-Induced Drought Stress

Phytotoxicity of silver nanoparticles on Vicia faba: Evaluation of particle size effects on photosynthetic performance and leaf gas exchange

The detection of hydrogen peroxide involved in plant virus infection by fluorescence spectroscopy

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