Reactive Inkjet Printing of Nylon Materials for Additive Manufacturing Applications

Srivatsan, T. S.; Sudarshan, T. S.

doi:10.1201/b19360-17

Cited by 3 publications

(2 citation statements)

References 34 publications

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“…The SEM images in figure 2 show the morphology of the PLA 3d printed test samples with increasing magnification. At 120 X magnification it is clear that the extruded PLA at standard (0.2 mm) resolution fused well; however, it can be noted that integrated filaments had a micron and sub-micron scale roughness (which as noted later may be beneficial for cell adhesion and growth when used in scaffolding applications (16,17)). The ridged structure likely results from the movement of the print head during extrusion and cooling, and may be affected by the retention of heat in the sample.…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 91%

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Cuiffo¹,

Snyder²,

Elliott³

et al. 2017

Preprint

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Polylactic Acid (PLA) is an organic polymer commonly used in fused deposition (FDM) printing and biomedical scaffolding that is biocompatible and immunologically inert. However, variations in source material quality and chemistry make it necessary to characterize the filament and determine potential changes in chemistry occurring as a result of the FDM process. We used several spectroscopic techniques, including laser confocal microscopy, Fourier-Transform Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 3 April 2017 doi:10.20944/preprints201704.0010.v1Peer-reviewed version available at Appl. Sci. 2017, 7, , 579; doi:10.3390/app7060579 Infrared (FTIR) spectroscopy and photoacousitc FTIR spectroscopy, Raman spectroscopy, and X-ray photoelectron Spectroscopy (XPS) in order to characterize both the bulk and surface chemistry of the source material and printed samples. Scanning Electron Microscopy (SEM) and Differential Scanning Calorimetry (DSC) were used to characterize morphology, crystallinity, and the glass transition temperature following printing. Analysis revealed calcium carbonatebased additives which were reacted with organic ligands and potentially trace metal impurities, both before and following printing. These additives became concentrated in voids in the printed structure. This finding is important for biomedical applications as carbonate will impact subsequent cell growth on printed tissue scaffolds. Results of chemical analysis also provided evidence of the hygroscopic nature of the source material and oxidation of the printed surface, and SEM imaging revealed micro and sub-micron scale roughness that will also impact potential applications.

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Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 91%

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Cuiffo¹,

Snyder²,

Elliott³

et al. 2017

Preprint

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“…Hydrogels containing muscle precursor cells have been widely used as bioinks in combination with support structures in thermoplastic polymers [274,275] and/or sacrificial materials [136,[276][277][278] to attain proper arrangement of cell-laden fibers capable of mimicking the native muscle tissue [264]. Different studies have investigated the propriety of GelMA hydrogel and its composites with various nanomaterials for SMTE [72,265,279,280].…”

Section: Skeletal Musclementioning

confidence: 99%

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

et al. 2021

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Engineering muscle cell alignment through 3D bioprinting

Mozetic

Giannitelli

Gori

et al. 2017

J Biomedical Materials Res

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Processing of hydrogels represents a main challenge for the prospective application of additive manufacturing (AM) to soft tissue engineering. Furthermore, direct manufacturing of tissue precursors with a cell density similar to native tissues has the potential to overcome the extensive in vitro culture required for conventional cell-seeded scaffolds seeking to fabricate constructs with tailored structural and functional properties. In this work, we present a simple AM methodology that exploits the thermoresponsive behavior of a block copolymer (Pluronic ) as a means to obtain good shape retention at physiological conditions and to induce cellular alignment. Pluronic/alginate blends have been investigated as a model system for the processing of C2C12 murine myoblast cell line. Interestingly, C2C12 cell model demonstrated cell alignment along the deposition direction, potentially representing a new avenue to tailor the resulting cell histoarchitecture during AM process. Furthermore, the fabricated constructs exhibited high cell viability, as well as a significantly improved expression of myogenic genes vs. conventional 2D cultures. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 2582-2588, 2017.

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Reactive Inkjet Printing of Nylon Materials for Additive Manufacturing Applications

Cited by 3 publications

References 34 publications

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Impact of the Fused Deposition (FDM) Printing Process on Polylactic Acid (PLA) Chemistry and Structure

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

Engineering muscle cell alignment through 3D bioprinting

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