Reactive Electrospinning of Cross-Linked Poly(2-hydroxyethyl methacrylate) Nanofibers and Elastic Properties of Individual Hydrogel Nanofibers in Aqueous Solutions

In this critical review, an overview is given on recent advances in the development and application of stimuliresponsive electrospun nanofibers.Please check this proof carefully. Our staff will not read it in detail after you have returned it. Translation errors between word-processor files and typesetting systems can occur so the whole proof needs to be read. Please pay particular attention to: tabulated material; equations; numerical data; figures and graphics; and references. If you have not already indicated the corresponding author(s) please mark their name(s) with an asterisk. Please e-mail a list of corrections or the PDF with electronic notes attached --do not change the text within the PDF file or send a revised manuscript.Please bear in mind that minor layout improvements, e.g. in line breaking, table widths and graphic placement, are routinely applied to the final version.Please note that, in the typefaces we use, an italic vee looks like this: n, and a Greek nu looks like this: n.We will publish articles on the web as soon as possible after receiving your corrections; no late corrections will be made.Please return your final corrections, where possible within 48 hours of receipt, by e-mail to: chemsocrev@rsc.org Reprints-Electronic (PDF) reprints will be provided free of charge to the corresponding author. Enquiries about purchasing paper reprints should be addressed via: http://www.rsc.org/publishing/journals/guidelines/paperreprints/. Costs for reprints are below: Stimuli-responsive electrospun nanofibers are gaining considerable attention as highly versatile tools which offer great potential in the biomedical field. In this critical review, an overview is given on recent advances made in the development and application of stimuli-responsive fibers. The specific features of these electrospun fibers are highlighted and discussed in view of the properties required for the diverse applications. Furthermore, several novel biomedical applications are discussed and the respective advantages and shortcomings inherent to stimuli-responsive electrospun fibers are addressed (136 references).

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“…ions and chemicals from a surrounding solution to enzymes and cells embedded within the hydrogel. 22 …”

Section: Stimuli-responsive Electrospun Fibersmentioning

confidence: 99%

Stimuli-responsive electrospun fibers and their applications

et al. 2011

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“…[64][65][66] Further, methacrylate-based copolymers have been electrospun to form nanofibrous coatings that can be crosslinked after formation. 67,68 We have recently reported on the electrospinning of several elements of a library of 120 poly(b-aminoester)s that were photo polymerized after formation 69 as well as novel photocrosslinkable and hydrolytically degradable elastomers. 70 Clearly, there exists a wide range of polymers that can be processed into the nanofibrous format.…”

Section: Figmentioning

confidence: 99%

“…For example, Kim and colleagues showed that cross-linked poly-HEMA nanofibers snap back into place after being distended with an AFM probe tip. 68 Tan and coworkers fixed one end of a nanofiber, and used the AFM cantilever to deform and measure force generated in PEO nanofibers. 100 Similarly, the AFM probe tip has been used to carry out three-point bending tests of individual fibers placed over a groove microetched into a silicon wafer.…”

Section: Single-fiber Mechanicsmentioning

confidence: 99%

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Mauck

Baker

Nerurkar

et al. 2009

Tissue Engineering Part B: Reviews

186

177

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Tissue engineering of fibrous tissues of the musculoskeletal system represents a considerable challenge because of the complex architecture and mechanical properties of the component structures. Natural healing processes in these dense tissues are limited as a result of the mechanically challenging environment of the damaged tissue and the hypocellularity and avascular nature of the extracellular matrix. When healing does occur, the ordered structure of the native tissue is replaced with a disorganized fibrous scar with inferior mechanical properties, engendering sites that are prone to re-injury. To address the engineering of such tissues, we and others have adopted a structurally motivated approach based on organized nanofibrous assemblies. These scaffolds are composed of ultrafine polymeric fibers that can be fabricated in such a way to recreate the structural anisotropy typical of fiber-reinforced tissues. This straight-and-narrow topography not only provides tailored mechanical properties, but also serves as a 3D biomimetic micropattern for directed tissue formation. This review describes the underlying technology of nanofiber production and focuses specifically on the mechanical evaluation and theoretical modeling of these structures as it relates to native tissue structure and function. Applying the same mechanical framework for understanding native and engineered fiber-reinforced tissues provides a functional method for evaluating the utility and maturation of these unique engineered constructs. We further describe several case examples where these principles have been put to test, and discuss the remaining challenges and opportunities in forwarding this technology toward clinical implementation.

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“…Also, the nonvolatile nature of the cross-linking agent prevents the use of the vapor-phase crosslinking method (29)(30)(31). Several studies have described various other methods of creating cross-linked fibers; such as using heat (32)(33)(34)(35)(36) or UV radiation (20,(37)(38)(39) to initiate the cross-linking reaction during or after the synthesis of the fibers. Heat and UV light, however, have known microbicidal properties and thus are not suitable for use in this study.…”

Section: Resultsmentioning

confidence: 99%

“…The ability to make nano-and microfibers from water-soluble polymers by electrospinning offers a way to create hydrogels with nano-and microstructures. If these mechanical and biocompatible properties are combined with the high surface-to-volume ratios of fibers, the hydrogel can be used as an efficient support material both for enzymes and for immobilization of microorganisms (20). Lee and Belcher (21), Salalha et al (22), and Gensheimer et al (23) had investigated electrospinning as a possible method of encapsulating both bacteria and bacterial viruses.…”

mentioning

confidence: 99%

Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

Liu

Rafailovich

Malal

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Although microbes have been used in industrial and niche applications for several decades, successful immobilization of microbes while maintaining their usefulness for any desired application has been elusive. Such a functionally bioactive system has distinct advantages over conventional batch and continuous-flow microbial reactor systems that are used in various biotechnological processes. This article describes the use of polyethylene oxide 99-polypropylene oxide 67-polyethylene oxide99 triblock polymer fibers, created via electrospinning, to encapsulate microbes of 3 industrially relevant genera, namely, Pseudomonas, Zymomonas, and Escherichia. The presence of bacteria inside the fibers was confirmed by fluorescence microscopy and SEM. Although the electrospinning process typically uses harsh organic solvents and extreme conditions that generally are harmful to bacteria, we describe techniques that overcome these limitations. The encapsulated microbes were viable for several months, and their metabolic activity was not affected by immobilization; thus they could be used in various applications. Furthermore, we have engineered a microbe-encapsulated cross-linked fibrous polymeric material that is insoluble. Also, the microbe-encapsulated active matrix permits efficient exchange of nutrients and metabolic products between the microorganism and the environment. The present results demonstrate the potential of the electrospinning technique for the encapsulation and immobilization of bacteria in the form of a synthetic biofilm, while retaining their metabolic activity. This study has wide-ranging implications in the engineering and use of novel bio-hybrid materials or biological thin-film catalysts.biofilm ͉ cross-linking ͉ immobilization ͉ microbial ͉ Zymomonas

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Reactive Electrospinning of Cross-Linked Poly(2-hydroxyethyl methacrylate) Nanofibers and Elastic Properties of Individual Hydrogel Nanofibers in Aqueous Solutions

Cited by 95 publications

References 22 publications

Stimuli-responsive electrospun fibers and their applications

Stimuli-responsive electrospun fibers and their applications

Engineering on the Straight and Narrow: The Mechanics of Nanofibrous Assemblies for Fiber-Reinforced Tissue Regeneration

Engineering of bio-hybrid materials by electrospinning polymer-microbe fibers

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